Insights from modeling metabolism and amoeboid cell motility in the immune system

Steijn, L. van (2021)

Doctoral Thesis

This thesis focuses on two processes involved in fighting infections: metabolism and immune cell motility and navigation.
Regarding metabolism, we present ZebraGEM 2.0, an improved whole-genome scale metabolic reconstruction for zebrafish, that we used to study zebrafish metabolism upon infection with Mycobacterium marinum integrating gene expression data from control and infected zebrafish larvae. The chapters focusing on cell motility in response to the environment, revolve around the question of how the environmental inputs of cell-matrix interactions, cell-sized obstacles and cell-signalling upon wounding shape and guide cell motility.

• Metabolism
• Genome scale metabolic reconstruction
• Cell motility
• Computational model
• Immune system
• Topotaxis
• Zebrafish

• All authors: Steijn, L. van
• Supervisor: Merks, R.M.H.; Spaink, H.P.; Verbeek, F.J.
• Committee: Duijn Schouten, F.A. van der; Doelman, A.; Meijer, A.H.; Lange, E.C.M. de; Textor, J.C.; Dupré, L.
• Qualification: Doctor (dr.)
• Awarding Institution: Mathematical Institute (MI), Faculty of Science, Leiden University
• Date: 2021-07-15
• ISBN (print): 9789463327664