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CRISPR/Cas-induced targeted mutagenesis with Agrobacterium mediated protein delivery
The RNA guided endonuclease based on the CRISPR/Cas system of Streptococcus pyogenus is a potent new tool for genome engineering in plants. Delivery of this system is usually done by transforming plants (and other organisms) with Agrobacterium tumefaciens which transfers a T-DNA encoding the required genes through its Type IV Secretion System (T4SS). Instead, here we report the delivery of the Cas9 protein of the CRISPR/Cas system of Streptococcus pyogenus through the T4SS of Agrobacterium to Nicotiana benthamiana and Saccharomyces cerevisiae.
- All authors
- Schmitz, D.J.
- Supervisor
- Hooykaas, P.J.J.
- Co-supervisor
- Pater, B.S. de
- Committee
- Vergunst, A.C.; Memelink, J.; Spaink, H.P.; Otten, L.
- Qualification
- Doctor (dr.)
- Awarding Institution
- Institute of Biology , Faculty of Science , Leiden University
- Date
- 2018-09-20