The rapid expansion of research on human gut microbiome in recent decades has
highlighted its role in human metabolism, immune regulation, and behavior.20 Despite
significant progress in deciphering how the gut microbiome affects human health and
disease, a long journey lies ahead to fully solve the puzzle. Combining multi-omics
analyses has become a trend to unravel the intricate relationship between the gut
microbiome and the human host. Among the omics techniques, as a direct readout of
phenotypes, metabolomics provides a snapshot reflecting the functional properties of
the gut microbiome at the molecular level. This emphasizes the crucial role of
metabolomics in revealing this complex relationship and underscores the needs for
advances in metabolomics techniques. In this thesis, by proposing strategies to address
the matrix effect in LC-ESI-MS-based analytical method, we advanced untargeted
metabolomics towards...
Show moreThe rapid expansion of research on human gut microbiome in recent decades has
highlighted its role in human metabolism, immune regulation, and behavior.20 Despite
significant progress in deciphering how the gut microbiome affects human health and
disease, a long journey lies ahead to fully solve the puzzle. Combining multi-omics
analyses has become a trend to unravel the intricate relationship between the gut
microbiome and the human host. Among the omics techniques, as a direct readout of
phenotypes, metabolomics provides a snapshot reflecting the functional properties of
the gut microbiome at the molecular level. This emphasizes the crucial role of
metabolomics in revealing this complex relationship and underscores the needs for
advances in metabolomics techniques. In this thesis, by proposing strategies to address
the matrix effect in LC-ESI-MS-based analytical method, we advanced untargeted
metabolomics towards quantitative analysis. The focus then shifted to deepening our
understanding of the interactions between the gut microbiome and CMA in early life
from a metabolomics perspective. Overall, the research in this thesis suggested that
several gut microbiome-involved metabolic pathways may play a role in the acquisition
of CM tolerance, and provided evidence that the fecal metabolome can serve as a
potential readout to reflect the impact of early synbiotic supplementation in infants.
These findings offered valuable insights into the relationship between the gut
microbiome and CMA, aiding future research in developing microbiome-targeted
strategies for the prevention and management of CMA in early life.
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