Design, synthesis, and evaluation of antigenic peptide conjugates containing Toll-like receptor agonists

Ende, T.C. van den (2023)

Doctoral Thesis

This thesis describes the design, synthesis, and immunological evaluation of varying (neo)antigenic peptide conjugates containing either a TLR2 or a TLR7 agonist. Chapter 2 discusses the synthesis of a library of UPam functionalized (neo)antigenic peptides. Chapter 3 discusses the optimization of UPam and investigates the current paradigms surrounding UPam. Chapter 4 presents a new simplified agonist for TLR2, namely mini-UPam, which is then conjugated to some neoantigenic peptides and evaluated on its immunological properties. Chapter 5 discusses the design and synthesis of TLR2 and TLR7 dual functionalized antigenic peptides.

• Toll-like receptor
• TLR2
• TLR7
• Neoantigenic peptide
• Synthetic peptide
• Peptide conjugate
• Peptide synthesis

• All authors: Ende, T.C. van den
• Supervisor: Ossendorp, F.A.; Marel, G.A. van der; Flippov, D.V.
• Committee: Ubbink, M.; Codee, J.D.C.; Overkleeft, H.S.; Kasteren, S.I. van; Geluk, A.; Buschow, S.I.
• Qualification: Doctor (dr.)
• Awarding Institution: Leiden Institute of Chemistry (LIC), Faculty of Science, Leiden University
• Date: 2023-02-21
• ISBN (print): 9789464589214