Leiden University Scholarly Publications

Persistent URL of this record https://hdl.handle.net/1887/3151637

In Collections

This item can be found in the following collections:

Kusumawardhani, H. (2021)

Solvent tolerance mechanisms in Pseudomonas putida

Doctoral Thesis
Bacterial biocatalysis constitutes a sustainable alternative for high-value chemicals production by enabling the utilization of renewable feedstocks. However, biobased production of
aromatic compounds and biopolymers requires a specialized microbial cell factory. Microbial hosts may experience cell toxicity caused by the solvent-like compounds that emerge as products, substrates or intermediates during the production process. Therefore, solvent tolerance is an essential trait for the microbial hosts used in biobased production of aromatic chemicals and biopolymers. The work described in this thesis focused on identifying and characterizing genes/gene clusters which are involved in conferring solvent tolerance trait in bacteria. Adaptability of P. putida S12 is dependent on the ability to cope with the high energy demand of solvent stress. The inherent metabolic flexibility of P. putida S12 has partly been developed through horizontally transferred traits, such as aromatic...Show moreBacterial biocatalysis constitutes a sustainable alternative for high-value chemicals production by enabling the utilization of renewable feedstocks. However, biobased production of
aromatic compounds and biopolymers requires a specialized microbial cell factory. Microbial hosts may experience cell toxicity caused by the solvent-like compounds that emerge as products, substrates or intermediates during the production process. Therefore, solvent tolerance is an essential trait for the microbial hosts used in biobased production of aromatic chemicals and biopolymers. The work described in this thesis focused on identifying and characterizing genes/gene clusters which are involved in conferring solvent tolerance trait in bacteria. Adaptability of P. putida S12 is dependent on the ability to cope with the high energy demand of solvent stress. The inherent metabolic flexibility of P. putida S12 has partly been developed through horizontally transferred traits, such as aromatic degradation pathways and solvent extrusion pumps.
Show less
Solvent tolerance
Industrial biotechnology
Genome engineering
Microbial cell factory
Bacterial biocatalysis
All authors
Kusumawardhani, H.
Supervisor
De Winde, J.H.
Co-supervisor
Hosseini, R.
Committee
Wezel, G.P. van; Briegel, A.; Wierckx, N.; Agler-Rosenbaum, M.; Heipieper, H.J.
Qualification
Doctor (dr.)
Awarding Institution
Institute of Biology Leiden (IBL), Faculty of Science, Leiden University
Date
2021-03-11
ISBN (print)
9789464164350

Funding

Sponsorship
This work is financed by Indonesian Endowment Fund for Education (LPDP), The Ministry of Finance, Indonesia (grant number 20150322022595)