Total synthesis of alginate and zwitterionic SP1 oligosaccharides

Zhang, Q. (2018)

Doctoral Thesis

The work in this thesis has been focused on two subjects. The first is the assembly of alginate oligosaccharides and the generation of building blocks for the enzymatic synthesis of alginate, and the second is the total synthesis of large fragments of the zwitterionic SP1
polysaccharide. With these fragments, details about alginate biosynthesis can be obtained through binding studies with biosynthesis enzymes, conjugate vaccines can be generated and binding studies with major histocompatibility complex II molecules can be studied.

• Alginate
• Zwitterionic SP1
• Oligosaccharides
• Synthesis
• Vaccine

• All authors: Zhang, Q.
• Supervisor: Marel, G.A. van der
• Co-supervisor: Codée, J.D.C.
• Committee: Yu, B.; Boons, G.J.; Walvoort, M.T.C.; Overkleeft, H.S.; Brouwer, J.
• Qualification: Doctor (dr.)
• Awarding Institution: Institute of Chemistry, Faculty of Science, Leiden University
• Date: 2018-09-06
• Sponsorship: European Research Council (ERC-2011-AdG-290836 ‘Chembiosphing’, to H.S.O)