Synthetic peptides, nucleic acids and molecular probes to study ADP-Ribosylation

Voorneveld, J. (2022)

Doctoral Thesis

This thesis presents the first synthetic peptides ADP-ribosylated on serine, threonine, tyrosine, arginine and cysteine. Besides synthetic peptides, this thesis discusses the first synthetic route towards ADP-ribosylated nucleic acids. Furthermore, two photoaffinity probes for PARP1 have been developed and assessed in living cells and two activity based probes have been synthesized, designed for CD38.

• ADP-ribosylation
• Solid phase peptide synthesis
• (ADP-ribosyl)hydrolase
• Poly(ADP-ribose)polymerases
• Post-translational modification
• Bioorthogonal chemistry

• All authors: Voorneveld, J.
• Supervisor: Fillipov, D.M.; Marel, G.A. van der
• Committee: Overkleeft, H.S.; Codée, J.D.C.; Fiedler, D.; Tate, E.; Jessen, H.J.
• Qualification: Doctor (dr.)
• Awarding Institution: Leiden Institute of Chemistry (LIC), Faculty of Science, Leiden University
• Date: 2022-09-08
• ISBN (print): 9789464583755