Peptide-based probes for protein N-Methyltransferases

Zhang, Y. (2022)

Doctoral Thesis

The work described in this thesis focuses on the development of linear or cyclized peptide probes against protein N-methyltransferases to characterize their specific binding behavior, providing further binding details for inhibitory activity study. The thesis not only describes the extended application to produce peptide-based transition states mimicking PRMT inhibitors but builds an LC-MS/MS method to evaluate CARM1 inhibition and activity.

• Peptide Probes
• Protein methyltransferases
• transition state mimics
• LC-MS/MS
• Peptidomimetics
• cyclized peptide inhibitors

• All authors: Zhang, Y.
• Supervisor: Martin, N.I.
• Co-supervisor: Haren, M.J. van
• Committee: Wezel, G.P. van; Heitman, L.H.; Walvoort, M.T.C.; Pomplun, S.J.; Jongkees, S.A.K.
• Qualification: Doctor (dr.)
• Awarding Institution: Institute of Biology Leiden (IBL), Faculty of Science, Leiden University
• Date: 2022-06-02