Analysis and annotation of DNA methylation in two nonhuman primate species using the Infinium Human Methylation 450K and EPIC BeadChips

Pichon, F.; Shen, Y.M.; Busato, F.; Jochems, S.P.; Jacquelin, B.; Grand, R. le; ... ; Tost, J. (2021)

Article / Letter to editor

Aim: Nonhuman primates are essential for research on many human diseases. The Infinium Human Methylation450/EPIC BeadChips are popular tools for the study of the methylation state across the human genome at affordable cost. Methods: We performed a precise evaluation and re-annotation of the BeadChip probes for the analysis of genome-wide DNA methylation patterns in rhesus macaques and African green monkeys through in silico analyses combined with functional validation by pyrosequencing. Results: Up to 165,847 of the 450K and 261,545 probes of the EPIC BeadChip can be reliably used. The annotation files are provided in a format compatible with a variety of standard bioinformatic pipelines. Conclusion: Our study will facilitate high-throughput DNA methylation analyses in Macaca mulatta and Chlorocebus sabaeus.

• 450K
• African green monkey
• annotation
• chlorocebus
• DNA methylation
• EPIC
• Infinium
• macaca
• microarray
• monkey
• rhesus macaque
• vervet

• All authors: Pichon, F.; Shen, Y.M.; Busato, F.; Jochems, S.P.; Jacquelin, B.; Grand, R. le; Deleuze, J.F.; Muller-Trutwin, M.; Tost, J.
• Date: 2021-01-20
• Journal: Epigenomics
• Volume: 13
• Issue: 03
• Pages: 169 - 186
• DOI: doi:10.2217/epi-2020-0200
• Link: https://www.futuremedicine.com/doi/pdf/10.2217/epi-2020-0200