Leiden University Scholarly Publications

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Dao, T.H.H. (2010)

Metabolic changes in Arabidopsis thaliana plants overexperssing chalcone synthase

Doctoral Thesis
The study has shown that it is possible to introduce the heterologous CHS gene in Arabidopsis thaliana and common multicopies of transgenes containing plants were obtained. Analysis of the change in metabolome of CHS transgenic plants, high expression transgenic lines can be identified by markers such as flavonoids and phenylpropanoids. It is also clear that UV-A/blue light stress does not further increase the levels of these marker compounds in CHS transgenic Arabidopsis plants, whereas in wild type plants such a treatment results in increased levels of these compounds, in fact similar to that in the transgenic plants. There are certain physiological limitations in the accumulation of certain products. This thesis starts with a review of the function of CHS in plants and especially in plant resistance (Chapter 2). Chapter 3 deals with the work on Agrobacterium-mediated transformation of heterologous chalcone synthase in Arabidopsis thaliana Col. 0. The effect of overexpression...Show moreThe study has shown that it is possible to introduce the heterologous CHS gene in Arabidopsis thaliana and common multicopies of transgenes containing plants were obtained. Analysis of the change in metabolome of CHS transgenic plants, high expression transgenic lines can be identified by markers such as flavonoids and phenylpropanoids. It is also clear that UV-A/blue light stress does not further increase the levels of these marker compounds in CHS transgenic Arabidopsis plants, whereas in wild type plants such a treatment results in increased levels of these compounds, in fact similar to that in the transgenic plants. There are certain physiological limitations in the accumulation of certain products. This thesis starts with a review of the function of CHS in plants and especially in plant resistance (Chapter 2). Chapter 3 deals with the work on Agrobacterium-mediated transformation of heterologous chalcone synthase in Arabidopsis thaliana Col. 0. The effect of overexpression of CHS on the transcriptional level is discribed in this chapter. The activity of the CHS enzyme in the transgenic plants is reported in Chapter 4. In Chapter 5 metabolic profiling of Arabidopsis thaliana using nuclear magnetic resonance spectroscopy (NMR) is described. In this chapter the primary and secondary metabolites of Arabidopsis thaliana Col. 0 which can be detected by NMR are reported. Chapter 6 reports the metabolic profiling of CHS transgenic Arabidopsis. Metabolomic changes upon UV-A/blue light treatment of Arabidopsis thaliana were investigated (Chapter 7). Chapter 8 deals with the study of the effect of the non-pesticide chemical, Benzo(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester (BTH) on the Arabidopsis metabolome. Finally, the general summary and discussion of thesis are given in Chapter 9.Show less
Arabidopsis
Chalcone synthase
Metabolomic
NMR
Transgenic plants
All authors
Dao, T.H.H.
Supervisor
Verpoorte, R.
Co-supervisor
Linthorst, H.J.M; Kim, H.K.
Qualification
Doctor (dr.)
Awarding Institution
Institute of Biology, Faculty of Science, Leiden University
Date
2010-02-18
ISBN (print)
9789090251349

Juridical information

Court
LEI Universiteit Leiden

Funding

Sponsorship
Vietnamese government