Developmental regulation and evolution of cAMP signalling in Dictyostelium

Alvarez-Curto, E. (2007)

Doctoral Thesis

Through evolution the social amoebas have developed mechanisms to adapt to environmental changes and ensure survival. This thesis explores the evolutionary origins of cAMP signalling and regulation of developmental decisions in the model organism Dictyostelium discoideum. It also shows the first molecular-based phylogeny of the Dictyostelids. Development in Dictyostelium is characterized by the formation of a multicellular structure, the fruiting body, with a well-defined temporal and spatial pattern. cAMP, normally used as intracellular second messenger, in Dictyostelium is used also as an extracellular signal (chemoattractant) to mediate cell movement and cell differentiation. The study of the different components that control the formation of a multicellular fruiting body at a molecular level and from an evolutionary perspective shows that extracellular cAMP signalling was originally developed to control fruiting body morphogenesis. Furthermore it reinforces the idea that Dictyostelium is a simple but yet robust model to study the origins of multicellularity. Do to cAMP being so prevalent in Dictyostelium development I have studied the regulation of cAMP production during particular developmental stages showing in this thesis novel roles for the adenylyl cyclases that produce cAMP and their specific patters of expression during development. A thorough pharmacological analysis of these enzymes is also present in this work.

• Adenylyl cyclase
• cAMP
• Development
• Dictyostelium
• Evolution
• Gene expression
• Pattern
• Phylogeny
• Signalling
• Spore

• All authors: Alvarez-Curto, E.
• Supervisor: Supervisor: Hooykaas P.J.J., Schaap P.
• Qualification: Doctor (dr.)
• Date: 2007-10-23