Streptomyces are multicellular, Gram-positive bacteria in the phylum of actinobacteria which produce a high amount of bioactive natural products of which the expression is tightly coordinated with... Show moreStreptomyces are multicellular, Gram-positive bacteria in the phylum of actinobacteria which produce a high amount of bioactive natural products of which the expression is tightly coordinated with the life cycle. This thesis shows the identification of S. roseifaciens, a novel species with an uncommon, verticillate spore morphology and a unique household of SsgA-like proteins. Analyses of the peptidoglycan composition show that S. coelicolor show a pattern of 3-3 cross-linking befitting a tip-growing organism and change in composition between vegetative mycelium and spores. Kitasatosporae carry meso-DAP in the peptidoglycan of vegetative mycelium and LL-DAP in the peptidoglycan of spores. In line with this difference, the peptidoglycan architecture of these two growth stages undergoes such radical changes that they would seem to be from different species. S. coelicolor is naturally vancomycin resistant, but the addition of D-alanine and disruption in a single gene increases vancomycin sensitivity by a thousandfold. A knockout mutant of the alanine racemase, alr, requires exogenous addition of D-alanine. The Alr crystal structure of S. coelicolor and the D-cycloserine producer S. lavendulae were compared as to look for possible mechanisms for D-cycloserine resistance. Show less
Tin electrodeposition applications have rapidly evolved in the past 25 years. Usage of tin coatings has advanced from being mainly used for corrosion protection and decorative purposes, to being... Show moreTin electrodeposition applications have rapidly evolved in the past 25 years. Usage of tin coatings has advanced from being mainly used for corrosion protection and decorative purposes, to being used in modern technology such in electronic devices, photovoltaic cells and Li-ion batteries. The new tin coating applications have also come with challenges that require the production of nanostructured deposits, multilayers coatings and composites. Furthermore, the need to reduce energy and source consumptions, and the implementation of more environment-friendly processes, require detailed and fundamental knowledge of the electrodeposition process.The emphasis throughout this thesis is therefore to obtain detailed mechanistic information of tin electrodeposition process.The experimental and theoretical work presented in this thesis attempts to understand the mechanism of tin electrodeposition, and the effect of electrolyte anions and naphthalene-based additives, during the early and subsequent stages of the process. Show less
This work sets out to investigate the form and function of the imperative in the Rigveda. The morphology (and, where relevant, etymology), syntax and semantics of the imperative are covered.The... Show moreThis work sets out to investigate the form and function of the imperative in the Rigveda. The morphology (and, where relevant, etymology), syntax and semantics of the imperative are covered.The work is divided into the following major parts:Introduction: The place of the imperative within the Rigvedic verbal system is discussed. A section is devoted to the addressees of the second and third person imperatives. Particular attention is paid to the meaning of the latter. The relationship between the imperative and the optative is also investigated, since on the face of it, some of the functions of the imperative - those which do not denote commands but rather wishes and entreaties - are shared between the two. Owing to the extreme rarity of the third-person optative - with the single exception of the form syāt - the conclusion of this section is that the imperative is the usual method of expressing not only commands, but also wishes and aspirations.The morphology of the imperative: All of the forms of the imperative are considered. There is a comprehensive section on stem-ablaut, on accentuation, as well as an individual treatment of each verb ending, and their contact forms in the case of the athematic stems. The imperatives in –tāt and –si are given special consideration, as well as a group of analogically created imperatives in -i such as yódhi.Aorist versus present imperative: This section discusses the relationship between the present and aorist imperatives. It shows that the aorist imperative is a moribund archaism at the time of the Rigveda - more so in the late tenth book - and that it functions as a metrical variant of the present imperative, with no functional difference existing between the two forms. An exception to this is in fixed formulae, in which the ancient, pre-Vedic difference between these forms is preserved.Index of attested forms: A treatment of every attested imperative form in the Rigveda. All attested imperative forms are noted, their grammatical classification, and their frequency. Many verbs are discussed in detail, and copious examples from the text are given. Show less
The Homeric adjectives θοῦρος and θοῦρις (gen. -ιδος) are normally glossed as ‘rushing, impetuous, furious’. While θοῦρις exclusively qualifies feminine nouns, no feminine form of θοῦρος is... Show moreThe Homeric adjectives θοῦρος and θοῦρις (gen. -ιδος) are normally glossed as ‘rushing, impetuous, furious’. While θοῦρις exclusively qualifies feminine nouns, no feminine form of θοῦρος is attested. What was the exact relationship between θοῦρος and θοῦρις? In this paper it is argued that θοῦρις is not the paradigmatic feminine of θοῦρος, but an artificial formation of epic Greek. It arose in the formula θούριδος ἀλκῆς due to the metrical constraints of epic hexameter, and subsequently ousted the original feminine of θοῦρος. In elaborating this scenario, I show that the basic meaning of θοῦρος and θοῦρις is ‘fierce’. Other instances of artificial change of inflection in the Homeric Kunstsprache are discussed, and it is argued that the mechanism underlying their creation is linguistic contamination. Show less
This book provides a description of Cuvok, a language spoken by about 10,000 people in the far north of Cameroon. The study of the linguistic elements addresses and provides an analysis of the... Show moreThis book provides a description of Cuvok, a language spoken by about 10,000 people in the far north of Cameroon. The study of the linguistic elements addresses and provides an analysis of the language through the examination of phonological and morphological aspects, as well as some notes on syntax and a bilingual lexicon.The issue of prosodies is central to the discussion of vowel segments. The harmonic domain of the word has a prominent place in the morpho-phonological discussion. The noun and the verb are the only grammatical categories that exhibit harmonic domains within which the features +palatalization and -palatalization are observed. All satellite elements of these two categories are either palatalized or non-palatalized depending on the opposition between the two phonemic vowels in the final position of the nominal or verbal complex. The perception of the Cuvok world is revealed through the study of prepositions, markers of temporal and spatial location. The expression of the four cardinal points shows a fixed orientation of the Tchouvok people.Particular emphasis was placed on the ethnolinguistic aspect, which deals with the role of the blacksmith as a key actor in the development of the society. This monographic section examines the role of the blacksmith in a society that is undergoing rapid change and is prey to new ideas and habits.The Cuvok grammar is a work that constitutes a decisive step towards the development and standardization of this language and hence of the central Chadic languages in general. Show less
Streptomyces are multicellular bacteria that grow as branched filaments and are best known for producing the majority of our antibiotics, many immunosuppressant and anticancer compounds.... Show moreStreptomyces are multicellular bacteria that grow as branched filaments and are best known for producing the majority of our antibiotics, many immunosuppressant and anticancer compounds. Unfortunately their multicellular life style creates many problems for efficient industrial production. In a bioreactor, depending on the environment and the genetics, it can grow quickly as dispersed mycelia or aggregate in slow growing pellets. Either morphology has advantages and disadvantages, which can be product specific. For my thesis I studied the mechanism by which these filaments can aggregate into dense pellets. I found a small gene cluster that produces poly-1,6-N-acetylglucosamine, a bacterial glue which binds neighboring cells and required for pellet formation in S. coelicolor. Subsequently we can use these genes to control the morphology of streptomycetes in a liquid environment, tailoring it for production. My work has given us new insights in the mechanims through which streptomycetes aggregate, but also has the potential to make streptomycetes a more favorable host for industial production. Show less
This book provides a complete analysis of synchronic CV -> VC metathesis in Amarasi, a language of western Timor. Metathesis and unmetathesis realise a paradigm of parallel forms, pairs of... Show moreThis book provides a complete analysis of synchronic CV -> VC metathesis in Amarasi, a language of western Timor. Metathesis and unmetathesis realise a paradigm of parallel forms, pairs of which occur to complement each other throughout the language.Metathesis in Amarasi is superficially associated with a bewildering array of disparate phonological processes including: vowel deletion, consonant deletion, consonant insertion and multiple kinds of vowel assimilation, any of which can (and do) vary by lect in their realisation. By proposing that Amarasi has an obligatory CVCVC foot in which C-slots can be empty, all these phonological processes can be straightforwardly derived from a single rule of metathesis and two associated phonological rules.Three kinds of metathesis can be identified in Amarasi: (i) Before vowel initial enclitics, roots must undergo metathesis, responding to the need to create a phonological boundary between a clitic host and enclitic. Such metathesis is phonologically conditioned. (ii) Metathesis occurs within the syntax to signal attributive modification. Such a metathesised form cannot occur at the end of a phrase and thus requires the presence of an unmetathesised form to complete it syntactically. (iii) In the discourse an unmetathesised form marks an unresolved event or situation. Such an unmetathesised form cannot occur in isolation and requires a metathesised form to achieve resolution.Metathesis in Amarasi is the central linguistic process around which linguistic structures are organised. Amarasi metatheses also reflect fundamental Timorese notions of societal and cosmic organisation. Alongside weaving and other performed activities, metathesis is an important linguistic marker of identity in a region obsessed with similarities and differences between different groups. The complementarity of Amarasi metathesis and unmetathesis within the syntax and within discourse reflects the Timorese division of the world into a series of mutually dependent binary and complementary pairs. As well as being the key which unlocks the structure of the language, metathesis is also a reflection of the structure of Amarasi society and culture. Show less
Eggelbusch, M.; Shi, A.D.; Broeksma, B.C.; Vazquez-Cruz, M.; Soares, M.N.; Wit, G.M.J. de; ... ; Wust, R.C.I. 2022
Background Systemic inflammation is associated with skeletal muscle atrophy and metabolic dysfunction. Although the nucleotide-binding oligomerization domain-like receptor family pyrin domain... Show moreBackground Systemic inflammation is associated with skeletal muscle atrophy and metabolic dysfunction. Although the nucleotide-binding oligomerization domain-like receptor family pyrin domain containing 3 (NLRP3) inflammasome contributes to cytokine production in immune cells, its role in skeletal muscle is poorly understood. Here, we studied the link between inflammation, NLRP3, muscle morphology, and metabolism in in vitro cultured C2C12 myotubes, independent of immune cell involvement.Methods Differentiated C2C12 myotubes were treated with lipopolysaccharide (LPS; 0, 10, and 100-200 ng/mL) to induce activation of the NLRP3 inflammasome with and without MCC950, a pharmacological inhibitor of NLRP3-induced IL-1 ss production. We assessed markers of the NLRP3 inflammasome, cell diameter, reactive oxygen species, and mitochondrial function.Results NLRP3 gene expression and protein concentrations increased in a time-dependent and dose-dependent manner. Intracellular IL-1 ss concentration significantly increased (P < 0.0001), but significantly less with MCC950 (P = 0.03), suggestive of moderate activation of the NLRP3 inflammasome in cultured myotubes upon LPS stimulation. LPS suppressed myotube growth after 24 h (P = 0.03), and myotubes remained smaller up to 72 h (P = 0.0009). Exposure of myotubes to IL-1 ss caused similar alterations in cell morphology, and MCC950 mitigated these LPS-induced differences in cell diameter. NLRP3 appeared to co-localize with mitochondria, more so upon exposure to LPS. Mitochondrial reactive oxygen species were higher after LPS (P = 0.03), but not after addition of MCC950. Myotubes had higher glycolytic rates, and mitochondria were more fragmented upon LPS exposure, which was not altered by MCC950 supplementation.Conclusions LPS- induced activation of the NLRP3 inflammasome in cultured myotubes contributes to morphological and metabolic alterations, likely due to its mitochondrial association. Show less
Many languages are said to possess “gender”, that is, a morphosyntactic system in which nouns induce formal marking on other words beyond the noun itself (adjectives, verbs, etc.). Gorwaa (gow;... Show moreMany languages are said to possess “gender”, that is, a morphosyntactic system in which nouns induce formal marking on other words beyond the noun itself (adjectives, verbs, etc.). Gorwaa (gow; South-Cushitic; Tanzania) possesses a gender system which is interrelated with number in a complex manner. Following the line of reasoning that biological (semantic) sex, grammatical (syntactic) gender, and (morphological) form-class are “interrelated but autonomous domains of linguistic generalization” set out in Harris’(1991) examination of Spanish, and establishing that number and gender are interrelated in a complex manner, this paper considers the morphophonological word-markers of Gorwaa, a language whose nominal morphology is considerably different from that of Spanish. Following a discussion of gender and number in Gorwaa, all word-markers and their associated gender and number values are identified. In addition to being a useful exercise in arranging the empirical data, this paper sheds light on some surprising surface patterns of a little-studied language. Show less
This thesis investigates the grammar of Sheko, an Omotic language spoken in southwest Ethiopia. The study presents an analysis of the phonology, morphology and syntax of the language, amply... Show moreThis thesis investigates the grammar of Sheko, an Omotic language spoken in southwest Ethiopia. The study presents an analysis of the phonology, morphology and syntax of the language, amply illustrated by examples. The supplements contain a sample of texts and an extensive word list. Among other things, the author discusses the interrelation between gender, definiteness and number in nominal morphology. Some nominal morphology also occurs on verbal forms. In the verb system, stem formation, aspectual and modal distinctions as well as stance marking are treated. The language has a rich array of complex verb constructions, such as medial clauses and serial verb constructions, next to different types of subordinate clauses. In relative clauses, a resumptive pronoun may occur before the antecedent. One of the divergent ways in which interrogatives are formed is by ‘subtractive morphology’, i.e. dropping off a grammatical element which is obligatorily present in declaratives. Furthermore, subject clitics attach to a variety of hosts in correlation to information structure and focus. These and a number of other issues make the work valuable for specialists of Omotic and Afroasiatic studies as well as for general linguists and anyone interested in the typology of language. Show less
Morphometric and morphological differences have been used extensively in the past to study domestication of the wolf and the origin of dogs. Certainly before genetic testing was performed, these... Show moreMorphometric and morphological differences have been used extensively in the past to study domestication of the wolf and the origin of dogs. Certainly before genetic testing was performed, these methods were the only means to diverge both groups. But still now, when aDNA cannot be extracted, morphometry and morphology are still important study methods to discern between wolves and dogs.Many of the historically claimed differences were based on studying rather low numbers of Specimens, and on comparisons with genetically isolated groups of dogs or breeds that had anatomical variants which diverge from the mean. This led to several claimed differences that needed rigorous re-evaluated, by investigating larger groups of specimens, both dogs and wolves, and more importantly, and if possible, Pleistocene wolves and the oldest archaeological dogs.We re-evaluated all important morphological and morphometric criteria published in the literature. Most are related to oral, mandibular and skull differences, but also difference in stature was reported. From all criteria we re-tested very few proved to be valuable. And those that show a difference, can often only be used to a limited extend, as only the extremes of these criteria are non-overlapping between groups. Show less
This study treats the verbal aspect system in Old Church Slavonic. A proper analysis of the aspect system in Old Church Slavonic can contribute to a better understanding of verbal aspect in the... Show moreThis study treats the verbal aspect system in Old Church Slavonic. A proper analysis of the aspect system in Old Church Slavonic can contribute to a better understanding of verbal aspect in the modern Slavic languages and the development of aspect in Slavic. In this study, it is demonstrated that the verbal aspect system in Old Church Slavonic can best be described if one categorizes the verbs into three main categories: perfective, imperfective and anaspectual. This differs from the traditional division into perfective and imperfective verbs only. To support this categorization, the study contains over 300 examples. The semantic analysis of these examples shows, amongst others, that within the typology of Slavic aspect, verbal aspect in Old Church Slavonic functions more like verbal aspect in the Western group of Slavic languages (e.g. Czech) than like that in the Eastern group (e.g. Russian). The Eastern group can therefore be regarded as the more innovative group, while the Western group is the more conservative. The study also discusses the role the imperfect tense possibly plays in the development of derived imperfective verbs and, through that, in the development of the perfective-imperfective aspect opposition in Slavic. Show less
In Dutch dialects, verbs and complementizers can bear different morphological affixes depending on which nominal element they agree with in their local syntactic domain. For instance, in a dialect... Show moreIn Dutch dialects, verbs and complementizers can bear different morphological affixes depending on which nominal element they agree with in their local syntactic domain. For instance, in a dialect such as tegelen Dutch, the complementizer agrees with the first conjunct of a coordinated subject, while in Lapscheure Dutch, the complementizer agrees with the coordinated subject as a whole. Using a vast array of new data on complementizer agreement, first conjunct agreement, agreement with pronouns, verbal agreement and subject doubling in Dutch dialects, this study argues that the interplay between syntax and morphology is more intricate than has hitherto been assumed. More precisely, it is shown that the syntactic component determines which local nominal elements qualify for agreement with the verb or complementizer, depending on the specificity of the affix. The analysis is extended to similar syntactic contexts in typologically different languages such as Irish, Hebrew, Finnish, Tsez and Arabic. Show less
