Bacterial biocatalysis constitutes a sustainable alternative for high-value chemicals production by enabling the utilization of renewable feedstocks. However, biobased production ofaromatic... Show moreBacterial biocatalysis constitutes a sustainable alternative for high-value chemicals production by enabling the utilization of renewable feedstocks. However, biobased production ofaromatic compounds and biopolymers requires a specialized microbial cell factory. Microbial hosts may experience cell toxicity caused by the solvent-like compounds that emerge as products, substrates or intermediates during the production process. Therefore, solvent tolerance is an essential trait for the microbial hosts used in biobased production of aromatic chemicals and biopolymers. The work described in this thesis focused on identifying and characterizing genes/gene clusters which are involved in conferring solvent tolerance trait in bacteria. Adaptability of P. putida S12 is dependent on the ability to cope with the high energy demand of solvent stress. The inherent metabolic flexibility of P. putida S12 has partly been developed through horizontally transferred traits, such as aromatic degradation pathways and solvent extrusion pumps. Show less
By investigating the interaction between different types of nucleases (i.e. nicking versus cleaving), donor DNA structures and target chromatin environments, this thesis provides important insights... Show moreBy investigating the interaction between different types of nucleases (i.e. nicking versus cleaving), donor DNA structures and target chromatin environments, this thesis provides important insights into how to improve the three crucial parameters of genome editing: efficiency, specificity and fidelity. The work presented in this thesis expand the range of possibilities for high-fidelity genetic manipulation of human cells. Show less
Holkers, M.; Cathomen, T.; Goncalves, M.A.F.V. 2014
