This thesis focuses on the application of lipid-based nanomedicine in drug delivery, including small molecular antitumor drugs and biomacromolecules including mRNA, and evaluates their biological... Show moreThis thesis focuses on the application of lipid-based nanomedicine in drug delivery, including small molecular antitumor drugs and biomacromolecules including mRNA, and evaluates their biological performance. We have modified liposomes and LNPs with fusogenic coiled-coil peptides to enhance the drug/mRNA delivery efficiency (Chapter 2-4), and also investigated how the lipid composition of LNPs influences the immune response (Chapter 5). Show less
This dissertation contains four works during my PhD. Different biomaterials have been designed based on coiled-coil peptides. These biomaterials have a range of applications, inclusing drug... Show moreThis dissertation contains four works during my PhD. Different biomaterials have been designed based on coiled-coil peptides. These biomaterials have a range of applications, inclusing drug delivery, cell sorting to cell-cell fusion. By knowledge-guideddesign and modification, coiled-coil induced membrane fusion systems are expected to achieve drug delivery in vivo. Finally, L-forms obtained by coiled-coil induced fusion of different strains are expected to be an ideal model for studying questions related to the origin of life and novel antibiotics. Show less
The aim of this thesis is to study cell-cell interactions and the development of an assay to explore and quantify the exchange of membrane compounds. In the first part the rate of lipid exchange... Show moreThe aim of this thesis is to study cell-cell interactions and the development of an assay to explore and quantify the exchange of membrane compounds. In the first part the rate of lipid exchange was studied, during both natural cell-cell contacts and prolonged enforced contacts. In the second part lipidated coiled-coil peptides were used to study the behaviour and potential fusogenic behaviour of these compounds on cell-wall-deficient bacteria. Show less
