In this thesis we investigated the ability of two-photon multifocal microscopy for single-molecule microscopy in live cells and organisms. Two-photon excitation combined with multifocal scanning... Show moreIn this thesis we investigated the ability of two-photon multifocal microscopy for single-molecule microscopy in live cells and organisms. Two-photon excitation combined with multifocal scanning has the potential to achieve, high (temporal) resolution imaging at a low background. Splitting the laser beam into multiple beamlets reduced laser power to all but eliminate photobleaching. The low background combined with the fast scan speeds and absence of photobleaching allowed us to measure single-particles and single-molecules in live zebrafish embryo's for long time periods. To the best of our knowledge, it is the first time that single-molecule molecules have been observed in a wide-field two-photon microscope. Show less
This thesis presents the results of a study on the interfaces of insulating oxides with and without the insertion of a magnetic layer. Such interfaces can host a two-dimensional electron liquid,... Show moreThis thesis presents the results of a study on the interfaces of insulating oxides with and without the insertion of a magnetic layer. Such interfaces can host a two-dimensional electron liquid, making the interface conducting, with a wealth of phenomena to study. In order to create such interfaces, layers of oxides such as lanthanum aluminate, lanthanum titanate, and rare earth titanates were grown on the surface of crystalline strontium titanate. The growth method was pulsed laser deposition, in which short laser pulses ablate a target of the required material. The transport properties of these systems were studied by applying an external voltage to the back surface of the insulating substrate. Such a gate voltage allows us to vary the amount of charge carriers at the interface. In this way we could investigate magnetic effects occurring in the charge transport and their connection to the superconducting properties of oxide interfaces. The work resulted in a deeper of understanding of the so-called anomalous Hall effect, the magnetoresistance behavior, the origin of a resistance minimum in the back-gate experiments, and magnetoresistance hysteresis in the superconducting state in the various systems which were studied. Show less
The genetic information of all living organisms is contained in their DNA. Cells modify the degree of DNA compaction by epigenetics, which largely determines what genes are read out and which genes... Show moreThe genetic information of all living organisms is contained in their DNA. Cells modify the degree of DNA compaction by epigenetics, which largely determines what genes are read out and which genes are transcriptionally silent. Despite decades of research into this mechanism, there is no consensus on how cells realize the various degrees of DNA compaction in vivo. Eukaryotes, such as humans, compact their DNA into higher-order structures called compact chromatin fibers. We characterize these fibers through a combination of single-molecule force spectroscopy techniques like magnetic tweezers, and rigid base pair Monte Carlo simulations. We show that, for instance, the length and sequence of the linker DNA, the DNA between adjacent nucleosomes, control the mechanical properties of chromatin fibers. Our measurements suggest the formation of more than one higher-order fiber structure. A deeper understanding of the chromatin fiber and its compaction mechanism is important because the dysfunction of such regulation results in various medical conditions such as the epigenetic disorder type 1 diabetes, fragile X syndrome, or various cancers. Show less
The theoretical explanation of cosmic acceleration is nowadays one of the biggest puzzles in cosmology. Within the standard cosmological model (LCDM) the expansion is sourced by the vacuum energy... Show moreThe theoretical explanation of cosmic acceleration is nowadays one of the biggest puzzles in cosmology. Within the standard cosmological model (LCDM) the expansion is sourced by the vacuum energy associatedto the Cosmological Constant L. Despite its simplicity, the Cosmological Constant presents various unresolved problems from both the theoretical and the observational side.However, even if we dismiss these puzzles, the study of theoretical alternatives to LCDM is still of primary importance. In fact, the wealth and quality of cosmological data that we are expecting for thenext decade will allow us to test gravity on cosmological scales with unprecedented accuracy. This will give us the chance to investigate many of our theoretical ideas and to assess the strength of the standard model of cosmology on the largest scales.In this thesis we present different approaches that we can adopt to study modifications of gravity by means of cosmology. Show less
This thesis contributes to studying primordial cosmology theories and their detectability in future observations. The first part of the thesis studies a class of inflation models with curved field... Show moreThis thesis contributes to studying primordial cosmology theories and their detectability in future observations. The first part of the thesis studies a class of inflation models with curved field spaces, which are typically motivated in high energy physics theories. The second part of the thesis focuses on one particularly important cosmological observable -- primordial non-Gaussianity, whose phenomenology may reveal new physics effects in the very early Universe. Show less
This thesis focuses on amyloid proteins, a class of proteins that convert into amyloid fibrils. Such proteins are of high interest because they are related to many of the neurodegenerative diseases... Show moreThis thesis focuses on amyloid proteins, a class of proteins that convert into amyloid fibrils. Such proteins are of high interest because they are related to many of the neurodegenerative diseases. In the brains of patients with neurodegenerative diseases, plaques of β-sheet amyloid aggregates are found, but the mechanism of their formation and their role vis-à-vis the disease are unknown. Aggregation is difficult to study because amyloids are intrinsically disordered proteins that lack an ordered structure in solution. Here we apply electron paramagnetic resonance (EPR) as a new technique to better understand the properties of amyloid oligomers and their formation. Show less
Mechanical interactions between cells and their environment play an important role in many biological processes. These interactions are often anisotropic in nature, but most mathematical models in... Show moreMechanical interactions between cells and their environment play an important role in many biological processes. These interactions are often anisotropic in nature, but most mathematical models in the field of cell mechanics describe cells as isotropic entities. In this thesis we theoretically study the role of anisotropic forces in cell mechanics, and compare our predictions to experimental data. Show less
Gold nanoparticles show surprisingly strong interactions with light in the visible range, which can be divided into scattering, absorption, and photoluminescence. When a nanoparticle absorbs light,... Show moreGold nanoparticles show surprisingly strong interactions with light in the visible range, which can be divided into scattering, absorption, and photoluminescence. When a nanoparticle absorbs light, the corresponding energy is converted to heat, which can affect the environment of the (hot) nanoparticle. This thesis uses scattering and photoluminescence to study the behaviour of optically heated single gold nanoparticles: it discusses the behaviour of single plasmonic vapour nanobubbles, which occur around highly heated nanoparticles immersed in a liquid, the detection of chirality in nano-objects through their absorption and the photothermal effect, the behaviour of gold nanoparticles under sub-picosecond pulsed excitation, and the temperature dependence of pulse-excited photoluminescence of such particles. Show less
