CD4(+)CD25(+)FOXP3(+) regulatory T (Treg) cells control immunological tolerance. Treg cells are generated in the thymus (tTreg) or in the periphery. Their superior lineage fidelity makes tTregs the... Show moreCD4(+)CD25(+)FOXP3(+) regulatory T (Treg) cells control immunological tolerance. Treg cells are generated in the thymus (tTreg) or in the periphery. Their superior lineage fidelity makes tTregs the preferred cell type for adoptive cell therapy (ACT). How human tTreg cells develop is incompletely understood. By combining single-cell transcriptomics and flow cytometry, we in this study delineated three major Treg developmental stages in the human thymus. At the first stage, which we propose to name pre-Treg I, cells still express lineage-inappropriate genes and exhibit signs of TCR signaling, presumably reflecting recognition of self-antigen. The subsequent pre-Treg II stage is marked by the sharp appearance of transcription factor FOXO1 and features induction of KLF2 and CCR7, in apparent preparation for thymic exit. The pre-Treg II stage can further be refined based on the sequential acquisition of surface markers CD31 and GPA33. The expression of CD45RA, finally, completes the phenotype also found on mature recent thymic emigrant Treg cells. Remarkably, the thymus contains a substantial fraction of recirculating mature effector Treg cells, distinguishable by expression of inflammatory chemokine receptors and absence of CCR7. The developmental origin of these cells is unclear and warrants caution when using thymic tissue as a source of stable cells for ACT. We show that cells in the major developmental stages can be distinguished using the surface markers CD1a, CD27, CCR7, and CD39, allowing for their viable isolation. These insights help identify fully mature tTreg cells for ACT and can serve as a basis for further mechanistic studies into tTreg development. Show less
Four artist-researchers associated with the Academy of Creative and Performing Arts, Leiden University discuss their manners of documenting and observing their performing selves. What role can... Show moreFour artist-researchers associated with the Academy of Creative and Performing Arts, Leiden University discuss their manners of documenting and observing their performing selves. What role can intuition play in research? Can self-reflection and performance coinhabit the same corporeal space? Although the participants share points of view, their practices, the materials they engage with and the intended outcomes differ. Show less
The Ig superfamily protein glycoprotein A33 (GPA33) has been implicated in immune dysregulation, but little is known about its expression in the immune compartment. Here, we comprehensively... Show moreThe Ig superfamily protein glycoprotein A33 (GPA33) has been implicated in immune dysregulation, but little is known about its expression in the immune compartment. Here, we comprehensively determined GPA33 expression patterns on human blood leukocyte subsets, using mass and flow cytometry. We found that GPA33 was expressed on fractions of B, dendritic, natural killer and innate lymphoid cells. Most prominent expression was found in the CD4(+) T cell compartment. Naive and CXCR5(+) regulatory T cells were GPA33(high), and naive conventional CD4(+) T cells expressed intermediate GPA33 levels. The expression pattern of GPA33 identified functional heterogeneity within the CD4(+) central memory T cell (Tcm) population. GPA33(+) CD4(+) Tcm cells were fully undifferentiated, bona fide Tcm cells that lack immediate effector function, whereas GPA33(-) Tcm cells exhibited rapid effector functions and may represent an early stage of differentiation into effector/effector memory T cells before loss of CD62L. Expression of GPA33 in conventional CD4(+) T cells suggests a role in localization and/or preservation of an undifferentiated state. These results form a basis to study the function of GPA33 and show it to be a useful marker to discriminate between different cellular subsets, especially in the CD4(+) T cell lineage. Show less
Opstelten, R.; Kivit, S. de; Slot, M.C.; Biggelaar, M. van den; Iwaszkiewicz-Grzes, D.; Gliwinski, M.; ... ; Amsen, D. 2020
FOXP3-expressing regulatory T (Treg) cells safeguard immunological tolerance. Treg cells can be generated during thymic development (called thymic Treg [tTreg] cells) or derived from mature... Show moreFOXP3-expressing regulatory T (Treg) cells safeguard immunological tolerance. Treg cells can be generated during thymic development (called thymic Treg [tTreg] cells) or derived from mature conventional CD4(+) T cells that underwent TGF-beta- mediated conversion in the periphery (called peripheral Treg [pTreg] cells). Murine studies have shown that tTreg cells exhibit strong lineage fidelity, whereas pTreg cells can revert into conventional CD4(+) T cells. Their stronger lineage commitment makes tTreg cells the safest cells to use in adoptive cell therapy, increasingly used to treat autoimmune and inflammatory disorders. Markers to distinguish human tTreg cells from pTreg cells have, however, not been found. Based on combined proteomic and transcriptomic approaches, we report that the Ig superfamily protein GPA33 is expressed on a subset of human Treg cells. GPA33 is acquired late during tTreg cell development but is not expressed on TGF-beta-induced Treg cells. GPA33 identifies Treg cells in human blood that lack the ability to produce effector cytokines (IL-2, IFN-gamma, IL-17), regardless of differentiation stage. GPA33(high) Treg cells universally express the transcription factor Helios that preferentially marks tTreg cells and can robustly and stably be expanded in vitro even without rapamycin. Expanded GPA33(high) Treg cells are suppressive, unable to produce proinflammatory cytokines, and exhibit the epigenetic modifications of the FOXP3 gene enhancer CNS2, necessary for indelible expression of this critical transcription factor. Our findings thus suggest that GPA33 identifies human tTreg cells and provide a strategy to isolate such cells for safer and more efficacious adoptive cell therapy. Show less
Despite most pianists' claims of historical deference and creative agency, their performances of Brahms's piano works are nothing like the early-recorded performances of the composer and his... Show moreDespite most pianists' claims of historical deference and creative agency, their performances of Brahms's piano works are nothing like the early-recorded performances of the composer and his students: gaps that are mediated by understandings of Brahms's Classical canonic identity, the performance norms that protect that identity, and those norms' underlying aesthetic ideology of control. This predication of Brahmsian identity on restraint leaves the composer and his students in a precarious situation, as their recordings evidence an approach that is governed by the inhibitions typically associated with Romanticism. This volume seeks to problematize understandings of Brahms's identity: by investigating the origins and vestiges of the aesthetic ideology of control; by analysing and copying the recordings of pianists in the composer's inner circle; and by applying these pianists' styles in ways that are just as disruptive to modern notions of Brahmsian identity as their early-recorded models. In so doing, a thoroughly Romantic performance style emerges that catalyses a fundamental shift in understanding as related to Brahms's identity; thereby opening up a new palette of expressive and technical resources, and both elucidating and narrowing persistent gaps between modern and early-recorded Brahms style, as well as between what performers believe, know, and ultimately do. Show less
