The EuroFlow PID consortium developed a set of flow cytometry tests for evaluation of patients with suspicion of primary immunodeficiency (PID). In this technical report we evaluate the performance... Show moreThe EuroFlow PID consortium developed a set of flow cytometry tests for evaluation of patients with suspicion of primary immunodeficiency (PID). In this technical report we evaluate the performance of the SCID-RTE tube that explores the presence of recent thymic emigrants (RTE) together with T-cell activation status and maturation stages and discuss its applicability in the context of the broader EuroFlow PID flow cytometry testing algorithm for diagnostic orientation of PID of the lymphoid system. We have analyzed peripheral blood cells of 26 patients diagnosed between birth and 2 years of age with a genetically defined primary immunodeficiency disorder: 15 severe combined immunodeficiency (SCID) patients had disease-causing mutations in RAG1 or RAG2 (n = 4, two of them presented with Omenn syndrome), IL2RG (n = 4, one of them with confirmed maternal engraftment), NHEJ1 (n = 1), CD3E (n = 1), ADA (n = 1), JAK3 (n = 3, two of them with maternal engraftment) and DCLRE1C (n = 1) and 11 other PID patients had diverse molecular defects [ZAP70 (n = 1), WAS (n = 2), PNP (n = 1), FOXP3 (n = 1), del22q11.2 (DiGeorge n = 4), CDC42 (n = 1) and FAS (n = 1)]. In addition, 44 healthy controls in the same age group were analyzed using the SCID-RTE tube in four EuroFlow laboratories using a standardized 8-color approach. RTE were defined as CD62L+CD45RO-HLA-DR-CD31+ and the activation status was assessed by the expression of HLA-DR+. Naive CD8+ T-lymphocytes and naive CD4+ T-lymphocytes were defined as CD62L+CD45RO-HLA-DR-. With the SCID-RTE tube, we identified patients with PID by low levels or absence of RTE in comparison to controls as well as low levels of naive CD4+ and naive CD8+ lymphocytes. These parameters yielded 100% sensitivity for SCID. All SCID patients had absence of RTE, including the patients with confirmed maternal engraftment or oligoclonally expanded T-cells characteristic for Omenn syndrome. Another dominant finding was the increased numbers of activated CD4+HLA-DR+ and CD8+HLA-DR+ lymphocytes. Therefore, the EuroFlow SCID-RTE tube together with the previously published PIDOT tube form a sensitive and complete cytometric diagnostic test suitable for patients suspected of severe PID (SCID or CID) as well as for children identified via newborn screening programs for SCID with low or absent T-cell receptor excision circles (TRECs). Show less
This article introduces the special issue ‘Online Publics in Muslim Southeast Asia: In Between Religious Politics and Popular Pious Practices’ by discussing prominent ap- proaches in the study... Show moreThis article introduces the special issue ‘Online Publics in Muslim Southeast Asia: In Between Religious Politics and Popular Pious Practices’ by discussing prominent ap- proaches in the study of media and the public sphere in light of the specific history of digital media’s rise in Muslim Southeast Asia. It focuses on earlier and current expres- sions of mobile and Islamic modernity as well as on changing moralities and forms of Islamic authority. Referencing the other contributions to this special issue, it particu- larly emphasizes the (discursive and visual) contestations and social dramas that take place in the region’s media spaces providing for a variety of Islamic forms, practices, and socialities that can best be grasped, the authors argue, by considering politics, the pious, and the popular not as separate, but as mutually constitutive domains. Show less
Since the early 1990s nasyid music has become widely popular among the Islamic youth of Indonesia and Malaysia. Imported from the Middle East, the verbal art attracts proponents particularly in... Show moreSince the early 1990s nasyid music has become widely popular among the Islamic youth of Indonesia and Malaysia. Imported from the Middle East, the verbal art attracts proponents particularly in universities, above all among student activists. However, the Middle East is not the sole role model; present-day nasyid music reveals the careful and often delicate mix of religion and pop that is currently so much debated throughout the Islamic world. Show less
Do the performing arts play a role in sexual selection? How does music influence mating practices in different cultures? an the performing arts create social settings where sexual relationships... Show moreDo the performing arts play a role in sexual selection? How does music influence mating practices in different cultures? an the performing arts create social settings where sexual relationships germinate and grow - even where sex is a disruptive force, an arena for competition and conflict? And if so, where does this power of the performing arts come from? Show less
Religion, more often than not, equates the arts of seduction - whether in traditional performances or popular music - with immoral behaviour. The status of music and dance in the Islamic world,... Show moreReligion, more often than not, equates the arts of seduction - whether in traditional performances or popular music - with immoral behaviour. The status of music and dance in the Islamic world, especially the fear of its sensuous powers, has been heatedly discussed in religious treatises; with its clean-cut performers and moral messages, nasyid, the Islamic boy-band music of Southeast Asia, seems to epitomise the art of no-seduction. Reality, however, is more complex, as Muslim pop music struggles to combine two competing powers - the eroticism of pop music and the persuasive power of religion. And especially when the female voice comes into play.... Show less
