A general overview of regulation of secondary metabolism in Pseudomonas species is given in Chapter 1. Several approaches were combined to identify novel genes involved in the regulation of PCN... Show moreA general overview of regulation of secondary metabolism in Pseudomonas species is given in Chapter 1. Several approaches were combined to identify novel genes involved in the regulation of PCN synthesis and to study their interactions with other regulators. Site-directed mutagenesis was used to test the hypothesis that rpoS is a regulatory gene of PCN synthesis (Chapter 2). To discover additional genes in the regulatory cascade, which already contains psrA and rpoS, a random DNA-fragment microarray of the PCL1391 genome was constructed and used for transcriptomics of the psrA and rpoS mutants (Chapter 3). A random mutagenesis approach resulted in the identification of pip, a novel gene that stimulates PCN production in PCL1391 (Chapter 4). Analyses on the role of Pip as a switch of PCN production depending on environmental conditions are described in Chapter 5. The results described in this thesis are summarized in Chapter 6, where in addition the regulatory network of PCN synthesis in P. chlororaphis PCL1391 is compared to regulatory networks of secondary metabolism in other Pseudomonas species. Show less
This study describes the effects of prominence and boundary on the temporal and melodic structures of two Indonesian languages, viz. Toba Batak, a stress language and Betawi Malay, a non-stress... Show moreThis study describes the effects of prominence and boundary on the temporal and melodic structures of two Indonesian languages, viz. Toba Batak, a stress language and Betawi Malay, a non-stress language. Experimental evidence shows that lengthening effects were more than twice as strong in the non-stress Betawi Malay as in Toba Batak. Durational prominence effects in Toba Batak are comparable to those found in western stress language at the word level, but not at the lower levels: stress syllables, especially consonants are hardly affected. Arguably, the use of duration as a stress correlate is restricted by the phonemic consonant contrast in the language. To compensate for this, prominence-related pitch movements, which are connected to the stressed syllable, occur in non-prominent as well as prominent words in Toba Batak. In Betawi Malay, prominence-related pitch movements are larger but vary considerably in shape and position; they serve to cue accents and boundaries, but not stress position. To determine the audible consequences of the native language for the production of Dutch stress, three perception experiments were run. The native language clearly affects the prosody of second-language speech. Toba Batak speakers of Dutch sound more acceptable to Dutch listeners than Betawi Malay speakers do. Show less
