Sheltering is an immediate human need and determines well-being andhealth. However, we face many challenges in providing homes and offices for allon this rapidly changing planet. In the 21st... Show moreSheltering is an immediate human need and determines well-being andhealth. However, we face many challenges in providing homes and offices for allon this rapidly changing planet. In the 21st century, climate change, resourcecrises, and population expansion will combine to exacerbate existingchallenges. We need to better understand and tackle these issues. Therefore,the overarching research question of this thesis is: What are the mainchallenges and opportunities in delivering decent shelters for nearly 10billion people in a warming and resource-scarce world? To this end, this thesismakes the first steps in exploring several key intersecting challenges relatedto resources scarcity, global warming, and natural hazards. Show less
Streptomycetes are soil-dwelling Gram-positive bacteria and can produce clinical drugs and adapt to environmental fluctuations through diverse morphological changes. The morphological development... Show moreStreptomycetes are soil-dwelling Gram-positive bacteria and can produce clinical drugs and adapt to environmental fluctuations through diverse morphological changes. The morphological development of streptomycetes features apical growth, which is determined by a tip-organizing center containing machinery for the synthesis of a cellulose-like glycan deposited at the cell surface. This thesis demonstrated that the cellulose-like glycan likely cellulose and the related synthesis machinery are unique in comparison with other Gram-negative cellulose synthesis systems. It showed that the synthesis of this glycan is performed by the glycosyltransferase ClsA and matured galactose-oxidase protein GlxA. Subsequently, the lytic polysaccharides monooxygenase LpmP and the glucanase ClsZ, adjacent to CslA/GlxA, cooperatively cleave peptidoglycan and create a passage to assist the exposure of this glycan on cell surface. In addition, this thesis also revealed coordination of polar growth determinants is performed by a stomatin-like protein StlP, which locates in the same gene cluster with CslA/GlxA/CslZ/LpmP. Oligomerization of StlP forms a fluid microdomain at hyphal tips, which spatially confines the whole tip organizing center. Furthermore, this thesis further showed that the cellulose-like glycan of Streptomyces coelicolor is responsible for the aggregation of clumps, which is distinct from Streptomyces lividans where this glycan mediates germlings’ aggregation. Show less
