Using a variety of anti-malaria tools has resulted in a steady decline of malaria in several endemic countries worldwide. An effective vaccine will be critical to halt malaria or even succeed to... Show moreUsing a variety of anti-malaria tools has resulted in a steady decline of malaria in several endemic countries worldwide. An effective vaccine will be critical to halt malaria or even succeed to final eradication. In that perspective, we studied the potential of whole sporozoite immunization by bites of P. falciparum infected mosquitoes under chemoprophylaxis (CPS). In this thesis we further explored this CPS model and assessed different immunizing doses, type of chemoprophylaxis and immunological determinants of disease and protection. We found a clear dose dependent efficacy, independent of type of chemoprophylaxis, found CD107a and CD8 T cells producing granzyme B related to protective immunity. In the field many genetically different strains circulate and a future vaccine should be able to cover multiple strains. We re-challenged volunteers with a different strain and found modest heterologously protection.We retrospectively assessed the parasitological dynamics and adverse events using a positive qPCR rather than thick smear and found reduced the clinical symptoms of malaria for volunteers after challenge.Successful malaria eradication will be more likely to be achieved with a multi-disciplinary approach. Additionally, sufficient and continuous funds will proof to be of tremendous necessity. Show less
This research investigates the impact of socioeconomic developments on the physical condition of medieval populations in Holland and Zeeland between AD 1000 and 1600 through the analysis of... Show more This research investigates the impact of socioeconomic developments on the physical condition of medieval populations in Holland and Zeeland between AD 1000 and 1600 through the analysis of human skeletal remains from three archaeological sites. In a brief period of time, this region went from being scarcely populated to an area characterised by expanding urban centres and flourishing trade systems. These large scale developments had an impact on the daily lives of medieval people. Focusing on several skeletal indicators of disease, activity, and diet, this research has studied the physical consequences of medieval socioeconomic developments from a hitherto unexplored perspective. Although differences are observed between the skeletal collections, the key finding is the absence of a marked distinction between town and country. The noted variations in skeletal indicators of disease, activity, and diet are minor and do not support the traditional idea that towns and villages in medieval Holland and Zeeland had become worlds apart. While urban living is frequently associated with negative consequences, this is not supported by this research. Especially in terms of disease, a more nuanced view is necessary. While the risks appear to have been different, one living environment cannot be considered better than the other. Show less
