Skip to main content
Leiden University
Scholarly Publications
Home
Submit
About
Select Collection
All collections
Academic speeches
Dissertations
Faculty of Archaeology
Faculty of Governance and Global Affairs
Faculty of Humanities
Faculty of Science
Faculty of Social and Behavioural Sciences
Leiden Journals, Conference Proceedings and Books
Leiden Law School
Leiden University Press
Medicine / Leiden University Medical Centre (LUMC)
Research output UL
Search box
Your Search
Enabled Filters
(-)
= pks plus E. coli
Sort Options
Date (year)
Author / Creator
Title
Refine Results
Availability
Open access
(1)
+
-
Search results
(1 - 1 of 1)
show grid
show list
save search
Klaver, W. de; Wit, M. de; Bolijn, A.; Tijssen, M.; Delis-van Diemen, P.; Lemmens, M.; ... ; Meijer, G.A.
2024
Polyketide synthase positive Escherichia coli one-time measurement in stool is not informative of colorectal cancer risk in a screening setting
Article / Letter to editor
open access
Environmental factors like the pathogenicity island
polyketide synthase positive (pks+) Escherichia coli
(
E. coli
) could have potential for risk stratification in colorectal cancer (CRC) screening...
Show more
Environmental factors like the pathogenicity island
polyketide synthase positive (pks+) Escherichia coli
(
E. coli
) could have potential for risk stratification in colorectal cancer (CRC) screening. The association between
pks+ E. coli
measured in fecal immunochemical test (FIT) samples and the detection of advanced neoplasia (AN) at colonoscopy was investigated. Biobanked FIT samples were analyzed for both presence of
E. coli
and
pks+ E. coli
and correlated with colonoscopy findings; 5020 CRC screening participants were included. Controls were participants in which no relevant lesion was detected because of FIT-negative results (cut-off ≥15 μg Hb/g feces), a negative colonoscopy, or a colonoscopy during which only a nonadvanced polyp was detected. Cases were participants with AN [CRC, advanced adenoma (AA), or advanced serrated polyp (ASP)]. Existing DNA isolation and quantitative polymerase chain reaction (qPCR) procedures were used for the detection of
E. coli
and
pks+ E. coli
in stool. A total of 4542 (90.2%) individuals were
E. coli
positive, and 1322 (26.2%) were
pks+ E. coli
positive. The prevalence of
E. coli
in FIT samples from individuals with AN was 92.9% compared to 89.7% in FIT samples of controls (
p
= 0.010). The prevalence of
pks+ E. coli
in FIT samples from individuals with AN (28.6%) and controls (25.9%) was not significantly different (
p
= 0.13). The prevalences of
pks+ E. coli
in FIT samples from individuals with CRC, AA, or ASP were 29.6%, 28.3%, and 32.1%, respectively. In conclusion, the prevalence of
pks+ E. coli
in a screening population was 26.2% and did not differ significantly between individuals with AN and controls. These findings disqualify the straightforward option of using a snapshot measurement of
pks+ E. coli
in FIT samples as a stratification biomarker for CRC risk. © 2024 The Authors.
The Journal of Pathology
published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.
Show less