For all organisms populating the Earth, motion is synonymous with life. In the living cell, protein molecules - its most important building blocks and work horses - are constantly on the move.... Show moreFor all organisms populating the Earth, motion is synonymous with life. In the living cell, protein molecules - its most important building blocks and work horses - are constantly on the move. Traditional static models are no longer sufficient for a thorough description of interactions among these biomolecules. Here we present a direct, experimental approach for assessing the dynamics of interactions between proteins, which for a long time has been an exclusive realm of theoretical studies. Show less
This thesis describes the peroxidase activity of the electron-transfer protein cytochrome c, and how it is controlled by the protein matrix. It is shown that unfolding cytochrome c has the effect... Show moreThis thesis describes the peroxidase activity of the electron-transfer protein cytochrome c, and how it is controlled by the protein matrix. It is shown that unfolding cytochrome c has the effect to significantly enhance its peroxidase activity of (up to several thousand-fold). This can be achieved by chaotropic agents or by detergents. The hydrogen peroxide-driven inactivation of the heme cofactor of cytochrome c is also characterised. Show less
