The book analyzes the manuscript on the Dutch language attributed to the Japanese scholar of Dutch Shizuki Tadao (1760 – 1806). It is often argued that the Japanese scholars’ knowledge of Dutch was... Show moreThe book analyzes the manuscript on the Dutch language attributed to the Japanese scholar of Dutch Shizuki Tadao (1760 – 1806). It is often argued that the Japanese scholars’ knowledge of Dutch was not particularly advanced, as they were mostly limited by their broken understanding of the contents of Dutch grammatical handbooks and dictionaries. The present book questions and investigates this claim with the goal of understanding the actual role played by Dutch sources in the learning of Dutch grammar.Shizuki can be considered as the first Japanese who studied the European theory of grammar. His representation of it is highly relevant within the history of linguistic thought. In the analysis of Shizuki’s manuscripts, this book concentrates on the representation of the categories of the parts of speech and of morphosyntactic phenomena related to verbs. While describing Dutch grammar, Shizuki often mentions other Japanese authors, like Ogyū Sorai and Motoori Norinaga. This book analyzes their works in relation to Shizuki’s manuscripts and his Dutch sources, contextualizing Shizuki’s theories and demonstrating their relationship to his sources. The book argues in favor of a new positioning of Shizuki and the other rangakusha within the scholarly environment of Early Modern Japan. Show less
Nabataean Aramaic contains a large number of loanwords from Arabic. Together with other evidence, this has been taken as an indication that the Nabataeans used Aramaic as a written language only,... Show moreNabataean Aramaic contains a large number of loanwords from Arabic. Together with other evidence, this has been taken as an indication that the Nabataeans used Aramaic as a written language only, while a Pre-Islamic variety of Arabic was their spoken language. Based on a comprehensive review of the evidence, however, this article concludes that both Arabic and Aramaic were in spoken use in the Nabataean Kingdom and Late Antique Northwest Arabia. Departing from this modified understanding of the linguistic status of Nabataean Aramaic, various features of Pre-Islamic Arabic are then examined based on the Nabataean evidence: the realisation of the voiceless sibilant /s/, nominal morphology, the reflexes of stem-final *y, verbal syntax, and the lexicon. Show less
Several groups within tribe Phyllantheae (Phyllanthaceae) formed, independently, an (obligate) pollination mutualism with Epicephala moths, which originally had been parasitic. In this pollination... Show moreSeveral groups within tribe Phyllantheae (Phyllanthaceae) formed, independently, an (obligate) pollination mutualism with Epicephala moths, which originally had been parasitic. In this pollination system, female moths actively collect pollen from staminate flowers and deposit it on the stigma of pistillate flowers, after which they place at least one egg in or against the ovary. The high pollination rate makes the system beneficial for the plants, whereas the larvae are provided with food (part of the developing seeds) and some protection against predation. Qualitative comparisons are made between non-moth-pollinated lineages, used as outgroups and various, independently moth-pollinated Phyllantheae clades, used as ingroups, thereby looking for parallel developments. The flowers of both sexes of various groups display similar, convergent morphological adaptations to the pollination system, likely to secure the obligate relationship and to improve efficiency. Sepals in both sexes, free or partly to highly connate, are commonly upright and form a narrow tube. The staminate flowers often have united, vertical stamens with the anthers along the androphore or on top of the androphore. Pistillate flowers generally reduce the stigmatic surface, either by making the stigmas shorter or by uniting them into a cone with a small opening at the top for pollen deposition. Less obvious is the reduction of the stigmatic papillae; these are often present in non-moth-pollinated taxa, but absent in the moth-pollinated species. The most diverging, parallel adaptations to moth pollination are currently found in the Palaeotropics, whereas in the Neotropics, some groups continue to also be pollinated by other insect groups and are morphologically less changed. Show less
The Homeric adjectives θοῦρος and θοῦρις (gen. -ιδος) are normally glossed as ‘rushing, impetuous, furious’. While θοῦρις exclusively qualifies feminine nouns, no feminine form of θοῦρος is... Show moreThe Homeric adjectives θοῦρος and θοῦρις (gen. -ιδος) are normally glossed as ‘rushing, impetuous, furious’. While θοῦρις exclusively qualifies feminine nouns, no feminine form of θοῦρος is attested. What was the exact relationship between θοῦρος and θοῦρις? In this paper it is argued that θοῦρις is not the paradigmatic feminine of θοῦρος, but an artificial formation of epic Greek. It arose in the formula θούριδος ἀλκῆς due to the metrical constraints of epic hexameter, and subsequently ousted the original feminine of θοῦρος. In elaborating this scenario, I show that the basic meaning of θοῦρος and θοῦρις is ‘fierce’. Other instances of artificial change of inflection in the Homeric Kunstsprache are discussed, and it is argued that the mechanism underlying their creation is linguistic contamination. Show less
The verbal class which is characterized by a suffix -nu- in the infinitive and -ne- in the present shows considerable dialectal diversity in Croatian and in the other western South Slavic languages... Show moreThe verbal class which is characterized by a suffix -nu- in the infinitive and -ne- in the present shows considerable dialectal diversity in Croatian and in the other western South Slavic languages. In many dialects, verbs belonging to this class have a suffix -ni- in the infinitive, aorist, participles and/or present. The aim of this paper is to explain how these dialectal forms and the relevant dialectal isoglosses have arisen. It argues that the allomorph -ni- < *-nywas originally found in the infinitive, while -nu- < *-nǫ- used to be restricted to the aorist. Show less
Eggelbusch, M.; Shi, A.D.; Broeksma, B.C.; Vazquez-Cruz, M.; Soares, M.N.; Wit, G.M.J. de; ... ; Wust, R.C.I. 2022
Background Systemic inflammation is associated with skeletal muscle atrophy and metabolic dysfunction. Although the nucleotide-binding oligomerization domain-like receptor family pyrin domain... Show moreBackground Systemic inflammation is associated with skeletal muscle atrophy and metabolic dysfunction. Although the nucleotide-binding oligomerization domain-like receptor family pyrin domain containing 3 (NLRP3) inflammasome contributes to cytokine production in immune cells, its role in skeletal muscle is poorly understood. Here, we studied the link between inflammation, NLRP3, muscle morphology, and metabolism in in vitro cultured C2C12 myotubes, independent of immune cell involvement.Methods Differentiated C2C12 myotubes were treated with lipopolysaccharide (LPS; 0, 10, and 100-200 ng/mL) to induce activation of the NLRP3 inflammasome with and without MCC950, a pharmacological inhibitor of NLRP3-induced IL-1 ss production. We assessed markers of the NLRP3 inflammasome, cell diameter, reactive oxygen species, and mitochondrial function.Results NLRP3 gene expression and protein concentrations increased in a time-dependent and dose-dependent manner. Intracellular IL-1 ss concentration significantly increased (P < 0.0001), but significantly less with MCC950 (P = 0.03), suggestive of moderate activation of the NLRP3 inflammasome in cultured myotubes upon LPS stimulation. LPS suppressed myotube growth after 24 h (P = 0.03), and myotubes remained smaller up to 72 h (P = 0.0009). Exposure of myotubes to IL-1 ss caused similar alterations in cell morphology, and MCC950 mitigated these LPS-induced differences in cell diameter. NLRP3 appeared to co-localize with mitochondria, more so upon exposure to LPS. Mitochondrial reactive oxygen species were higher after LPS (P = 0.03), but not after addition of MCC950. Myotubes had higher glycolytic rates, and mitochondria were more fragmented upon LPS exposure, which was not altered by MCC950 supplementation.Conclusions LPS- induced activation of the NLRP3 inflammasome in cultured myotubes contributes to morphological and metabolic alterations, likely due to its mitochondrial association. Show less
Stel, W. van der; Yang, H.; Le Dévédec, S.E.; Water, B. van de; Beltman, J.B.; Danen, E.H.J. 2022
Cells can adjust their mitochondrial morphology by altering the balance between mitochondrial fission and fusion to adapt to stressful conditions. The connection between a chemical perturbation,... Show moreCells can adjust their mitochondrial morphology by altering the balance between mitochondrial fission and fusion to adapt to stressful conditions. The connection between a chemical perturbation, changes in mitochondrial function, and altered mitochondrial morphology is not well understood. Here, we made use of high-throughput high-content confocal microscopy to assess the effects of distinct classes of oxidative phosphorylation (OXPHOS) complex inhibitors on mitochondrial parameters in a concentration and time resolved manner. Mitochondrial morphology phenotypes were clustered based on machine learning algorithms and mitochondrial integrity patterns were mapped. In parallel, changes in mitochondrial membrane potential (MMP), mitochondrial and cellular ATP levels, and viability were microscopically assessed. We found that inhibition of MMP, mitochondrial ATP production, and oxygen consumption rate (OCR) using sublethal concentrations of complex I and III inhibitors did not trigger mitochondrial fragmentation. Instead, complex V inhibitors that suppressed ATP and OCR but increased MMP provoked a more fragmented mitochondrial morphology. In agreement, complex V but not complex I or III inhibitors triggered proteolytic cleavage of the mitochondrial fusion protein, OPA1. The relation between increased MMP and fragmentation did not extend beyond OXPHOS complex inhibitors: increasing MMP by blocking the mPTP pore did not lead to OPA1 cleavage or mitochondrial fragmentation and the OXPHOS uncoupler FCCP was associated with OPA1 cleavage and MMP reduction. Altogether, our findings connect vital mitochondrial functions and phenotypes in a high-throughput high-content confocal microscopy approach that help understanding of chemical-induced toxicity caused by OXPHOS complex perturbing chemicals. Show less
This book provides a description of Cuvok, a language spoken by about 10,000 people in the far north of Cameroon. The study of the linguistic elements addresses and provides an analysis of the... Show moreThis book provides a description of Cuvok, a language spoken by about 10,000 people in the far north of Cameroon. The study of the linguistic elements addresses and provides an analysis of the language through the examination of phonological and morphological aspects, as well as some notes on syntax and a bilingual lexicon.The issue of prosodies is central to the discussion of vowel segments. The harmonic domain of the word has a prominent place in the morpho-phonological discussion. The noun and the verb are the only grammatical categories that exhibit harmonic domains within which the features +palatalization and -palatalization are observed. All satellite elements of these two categories are either palatalized or non-palatalized depending on the opposition between the two phonemic vowels in the final position of the nominal or verbal complex. The perception of the Cuvok world is revealed through the study of prepositions, markers of temporal and spatial location. The expression of the four cardinal points shows a fixed orientation of the Tchouvok people.Particular emphasis was placed on the ethnolinguistic aspect, which deals with the role of the blacksmith as a key actor in the development of the society. This monographic section examines the role of the blacksmith in a society that is undergoing rapid change and is prey to new ideas and habits.The Cuvok grammar is a work that constitutes a decisive step towards the development and standardization of this language and hence of the central Chadic languages in general. Show less
Attested in cuneiform, hieroglyphic and alphabetic texts dating to the first two millennia BCE, the Anatolian branch of the Indo-European language family is intriguing already by itself. But... Show moreAttested in cuneiform, hieroglyphic and alphabetic texts dating to the first two millennia BCE, the Anatolian branch of the Indo-European language family is intriguing already by itself. But Anatolian is also of central importance for the reconstruction of Proto-Indo-European, the last common ancestor of the Indo-European language family. Not only is it the earliest attested branch, it has also long been suspected that Anatolian reflects an earlier stage of the proto-language than that underlying the rest of the family.Focusing on the three best-attested Anatolian languages, Hittite, Luwian and Lycian, this book aims to further our understanding of Anatolian, and by extension Proto-Indo-European, by offering in-depth analyses of essential issues in Anatolian historical morphology and semantics. Various well-known as well as several newly adduced topics are scrutinized to determine whether the innovations leading to the discrepancies with the rest of Indo-European took place on the Anatolian or on the non-Anatolian side.The present study suggests that Anatolian is in many respects closer to the ancestor of the other Indo-European languages than is often claimed. Nevertheless, the investigation has also led to new evidence in favor of the hypothesis that Anatolian was the first branch to split off from the family. Show less
Tin electrodeposition applications have rapidly evolved in the past 25 years. Usage of tin coatings has advanced from being mainly used for corrosion protection and decorative purposes, to being... Show moreTin electrodeposition applications have rapidly evolved in the past 25 years. Usage of tin coatings has advanced from being mainly used for corrosion protection and decorative purposes, to being used in modern technology such in electronic devices, photovoltaic cells and Li-ion batteries. The new tin coating applications have also come with challenges that require the production of nanostructured deposits, multilayers coatings and composites. Furthermore, the need to reduce energy and source consumptions, and the implementation of more environment-friendly processes, require detailed and fundamental knowledge of the electrodeposition process.The emphasis throughout this thesis is therefore to obtain detailed mechanistic information of tin electrodeposition process.The experimental and theoretical work presented in this thesis attempts to understand the mechanism of tin electrodeposition, and the effect of electrolyte anions and naphthalene-based additives, during the early and subsequent stages of the process. Show less
This dissertation presents a description of Mankanya, an Atlantic language spoken by about 65 000 speakers in Guinea-Bissau, Senegal and the Gambia. It includes a sketch of the phonology and a... Show moreThis dissertation presents a description of Mankanya, an Atlantic language spoken by about 65 000 speakers in Guinea-Bissau, Senegal and the Gambia. It includes a sketch of the phonology and a detailed description of the morphology and syntax of the language. Some aspects of discourse level structure are also discussed and two interlinearised sample texts are included.Mankanya has a rich morphology with both nominal and verbal inflection, and a range of derivative morphemes. Like many other Atlantic languages, nouns can be grouped into classes based on the agreement of the inflections between nouns and their modifiers. Verbs have prefixes that agree with the subject. Though some verbal affixes indicate different aspects, most distinctions of tense, aspect and mode are made by using verbal auxiliaries. Clause chaining is possible with reduced subject agreement if the subject is unchanged. Where the subject does change a different subject marker is often used.A Grammar of Mankanya will be of interest for those studying of Atlantic languages, as well a resource for wider typological comparison. Show less
The Bantu languages show much variation in object marking, two parameters being (1) their behaviour in ditransitives (symmetric or asymmetric) and (2) the number of object markers allowed (single... Show moreThe Bantu languages show much variation in object marking, two parameters being (1) their behaviour in ditransitives (symmetric or asymmetric) and (2) the number of object markers allowed (single or multiple). This paper reveals that a combination of these parameter settings in a sample of 50+ Bantu languages results in an almost-gap, the AWSOM correlation: “asymmetry wants single object marking”. A Minimalist featural analysis is presented of Bantu object marking as agreement with a defective goal (van der Wal 2015) and parametric variation in the distribution of 𝜙 features on low functional heads (e.g. Appl) accounts for both the AWSOM and Sambaa as the one exception to the AWSOM. Show less
Morphometric and morphological differences have been used extensively in the past to study domestication of the wolf and the origin of dogs. Certainly before genetic testing was performed, these... Show moreMorphometric and morphological differences have been used extensively in the past to study domestication of the wolf and the origin of dogs. Certainly before genetic testing was performed, these methods were the only means to diverge both groups. But still now, when aDNA cannot be extracted, morphometry and morphology are still important study methods to discern between wolves and dogs.Many of the historically claimed differences were based on studying rather low numbers of Specimens, and on comparisons with genetically isolated groups of dogs or breeds that had anatomical variants which diverge from the mean. This led to several claimed differences that needed rigorous re-evaluated, by investigating larger groups of specimens, both dogs and wolves, and more importantly, and if possible, Pleistocene wolves and the oldest archaeological dogs.We re-evaluated all important morphological and morphometric criteria published in the literature. Most are related to oral, mandibular and skull differences, but also difference in stature was reported. From all criteria we re-tested very few proved to be valuable. And those that show a difference, can often only be used to a limited extend, as only the extremes of these criteria are non-overlapping between groups. Show less
Streptomyces are multicellular, Gram-positive bacteria in the phylum of actinobacteria which produce a high amount of bioactive natural products of which the expression is tightly coordinated with... Show moreStreptomyces are multicellular, Gram-positive bacteria in the phylum of actinobacteria which produce a high amount of bioactive natural products of which the expression is tightly coordinated with the life cycle. This thesis shows the identification of S. roseifaciens, a novel species with an uncommon, verticillate spore morphology and a unique household of SsgA-like proteins. Analyses of the peptidoglycan composition show that S. coelicolor show a pattern of 3-3 cross-linking befitting a tip-growing organism and change in composition between vegetative mycelium and spores. Kitasatosporae carry meso-DAP in the peptidoglycan of vegetative mycelium and LL-DAP in the peptidoglycan of spores. In line with this difference, the peptidoglycan architecture of these two growth stages undergoes such radical changes that they would seem to be from different species. S. coelicolor is naturally vancomycin resistant, but the addition of D-alanine and disruption in a single gene increases vancomycin sensitivity by a thousandfold. A knockout mutant of the alanine racemase, alr, requires exogenous addition of D-alanine. The Alr crystal structure of S. coelicolor and the D-cycloserine producer S. lavendulae were compared as to look for possible mechanisms for D-cycloserine resistance. Show less
In this thesis I discuss Hittite verbs and formations that contain or may contain a nasal infix as well as nu-verbs, in order to outline the development of these types from PIE to Hittite and... Show moreIn this thesis I discuss Hittite verbs and formations that contain or may contain a nasal infix as well as nu-verbs, in order to outline the development of these types from PIE to Hittite and establish the extent to which the Hittite nasal verbs are related to comparable formations in the other Indo-European languages. Apart from treating the formal and etymological aspects of these verbs, this study also focuses on the semantics of the nasal affixes. In Hittitological literature they are often assumed to have a causative or a factitive meaning, but these functions do not cover the semantics of all the verbs with these affixes: there are infixed and nu-verbs that are clearly not causative, in fact there are even several intransitive nu-verbs. In this thesis it is therefore argued that the best solution to embrace the polysemy of nasal affixes is to assume that the underlying function of these morphemes is raising in transitivity. Show less
Streptomyces are multicellular bacteria that grow as branched filaments and are best known for producing the majority of our antibiotics, many immunosuppressant and anticancer compounds.... Show moreStreptomyces are multicellular bacteria that grow as branched filaments and are best known for producing the majority of our antibiotics, many immunosuppressant and anticancer compounds. Unfortunately their multicellular life style creates many problems for efficient industrial production. In a bioreactor, depending on the environment and the genetics, it can grow quickly as dispersed mycelia or aggregate in slow growing pellets. Either morphology has advantages and disadvantages, which can be product specific. For my thesis I studied the mechanism by which these filaments can aggregate into dense pellets. I found a small gene cluster that produces poly-1,6-N-acetylglucosamine, a bacterial glue which binds neighboring cells and required for pellet formation in S. coelicolor. Subsequently we can use these genes to control the morphology of streptomycetes in a liquid environment, tailoring it for production. My work has given us new insights in the mechanims through which streptomycetes aggregate, but also has the potential to make streptomycetes a more favorable host for industial production. Show less
This study treats the verbal aspect system in Old Church Slavonic. A proper analysis of the aspect system in Old Church Slavonic can contribute to a better understanding of verbal aspect in the... Show moreThis study treats the verbal aspect system in Old Church Slavonic. A proper analysis of the aspect system in Old Church Slavonic can contribute to a better understanding of verbal aspect in the modern Slavic languages and the development of aspect in Slavic. In this study, it is demonstrated that the verbal aspect system in Old Church Slavonic can best be described if one categorizes the verbs into three main categories: perfective, imperfective and anaspectual. This differs from the traditional division into perfective and imperfective verbs only. To support this categorization, the study contains over 300 examples. The semantic analysis of these examples shows, amongst others, that within the typology of Slavic aspect, verbal aspect in Old Church Slavonic functions more like verbal aspect in the Western group of Slavic languages (e.g. Czech) than like that in the Eastern group (e.g. Russian). The Eastern group can therefore be regarded as the more innovative group, while the Western group is the more conservative. The study also discusses the role the imperfect tense possibly plays in the development of derived imperfective verbs and, through that, in the development of the perfective-imperfective aspect opposition in Slavic. Show less