Nabataean Aramaic contains a large number of loanwords from Arabic. Together with other evidence, this has been taken as an indication that the Nabataeans used Aramaic as a written language only,... Show moreNabataean Aramaic contains a large number of loanwords from Arabic. Together with other evidence, this has been taken as an indication that the Nabataeans used Aramaic as a written language only, while a Pre-Islamic variety of Arabic was their spoken language. Based on a comprehensive review of the evidence, however, this article concludes that both Arabic and Aramaic were in spoken use in the Nabataean Kingdom and Late Antique Northwest Arabia. Departing from this modified understanding of the linguistic status of Nabataean Aramaic, various features of Pre-Islamic Arabic are then examined based on the Nabataean evidence: the realisation of the voiceless sibilant /s/, nominal morphology, the reflexes of stem-final *y, verbal syntax, and the lexicon. Show less
Several groups within tribe Phyllantheae (Phyllanthaceae) formed, independently, an (obligate) pollination mutualism with Epicephala moths, which originally had been parasitic. In this pollination... Show moreSeveral groups within tribe Phyllantheae (Phyllanthaceae) formed, independently, an (obligate) pollination mutualism with Epicephala moths, which originally had been parasitic. In this pollination system, female moths actively collect pollen from staminate flowers and deposit it on the stigma of pistillate flowers, after which they place at least one egg in or against the ovary. The high pollination rate makes the system beneficial for the plants, whereas the larvae are provided with food (part of the developing seeds) and some protection against predation. Qualitative comparisons are made between non-moth-pollinated lineages, used as outgroups and various, independently moth-pollinated Phyllantheae clades, used as ingroups, thereby looking for parallel developments. The flowers of both sexes of various groups display similar, convergent morphological adaptations to the pollination system, likely to secure the obligate relationship and to improve efficiency. Sepals in both sexes, free or partly to highly connate, are commonly upright and form a narrow tube. The staminate flowers often have united, vertical stamens with the anthers along the androphore or on top of the androphore. Pistillate flowers generally reduce the stigmatic surface, either by making the stigmas shorter or by uniting them into a cone with a small opening at the top for pollen deposition. Less obvious is the reduction of the stigmatic papillae; these are often present in non-moth-pollinated taxa, but absent in the moth-pollinated species. The most diverging, parallel adaptations to moth pollination are currently found in the Palaeotropics, whereas in the Neotropics, some groups continue to also be pollinated by other insect groups and are morphologically less changed. Show less
The Homeric adjectives θοῦρος and θοῦρις (gen. -ιδος) are normally glossed as ‘rushing, impetuous, furious’. While θοῦρις exclusively qualifies feminine nouns, no feminine form of θοῦρος is... Show moreThe Homeric adjectives θοῦρος and θοῦρις (gen. -ιδος) are normally glossed as ‘rushing, impetuous, furious’. While θοῦρις exclusively qualifies feminine nouns, no feminine form of θοῦρος is attested. What was the exact relationship between θοῦρος and θοῦρις? In this paper it is argued that θοῦρις is not the paradigmatic feminine of θοῦρος, but an artificial formation of epic Greek. It arose in the formula θούριδος ἀλκῆς due to the metrical constraints of epic hexameter, and subsequently ousted the original feminine of θοῦρος. In elaborating this scenario, I show that the basic meaning of θοῦρος and θοῦρις is ‘fierce’. Other instances of artificial change of inflection in the Homeric Kunstsprache are discussed, and it is argued that the mechanism underlying their creation is linguistic contamination. Show less
The verbal class which is characterized by a suffix -nu- in the infinitive and -ne- in the present shows considerable dialectal diversity in Croatian and in the other western South Slavic languages... Show moreThe verbal class which is characterized by a suffix -nu- in the infinitive and -ne- in the present shows considerable dialectal diversity in Croatian and in the other western South Slavic languages. In many dialects, verbs belonging to this class have a suffix -ni- in the infinitive, aorist, participles and/or present. The aim of this paper is to explain how these dialectal forms and the relevant dialectal isoglosses have arisen. It argues that the allomorph -ni- < *-nywas originally found in the infinitive, while -nu- < *-nǫ- used to be restricted to the aorist. Show less
Eggelbusch, M.; Shi, A.D.; Broeksma, B.C.; Vazquez-Cruz, M.; Soares, M.N.; Wit, G.M.J. de; ... ; Wust, R.C.I. 2022
Background Systemic inflammation is associated with skeletal muscle atrophy and metabolic dysfunction. Although the nucleotide-binding oligomerization domain-like receptor family pyrin domain... Show moreBackground Systemic inflammation is associated with skeletal muscle atrophy and metabolic dysfunction. Although the nucleotide-binding oligomerization domain-like receptor family pyrin domain containing 3 (NLRP3) inflammasome contributes to cytokine production in immune cells, its role in skeletal muscle is poorly understood. Here, we studied the link between inflammation, NLRP3, muscle morphology, and metabolism in in vitro cultured C2C12 myotubes, independent of immune cell involvement.Methods Differentiated C2C12 myotubes were treated with lipopolysaccharide (LPS; 0, 10, and 100-200 ng/mL) to induce activation of the NLRP3 inflammasome with and without MCC950, a pharmacological inhibitor of NLRP3-induced IL-1 ss production. We assessed markers of the NLRP3 inflammasome, cell diameter, reactive oxygen species, and mitochondrial function.Results NLRP3 gene expression and protein concentrations increased in a time-dependent and dose-dependent manner. Intracellular IL-1 ss concentration significantly increased (P < 0.0001), but significantly less with MCC950 (P = 0.03), suggestive of moderate activation of the NLRP3 inflammasome in cultured myotubes upon LPS stimulation. LPS suppressed myotube growth after 24 h (P = 0.03), and myotubes remained smaller up to 72 h (P = 0.0009). Exposure of myotubes to IL-1 ss caused similar alterations in cell morphology, and MCC950 mitigated these LPS-induced differences in cell diameter. NLRP3 appeared to co-localize with mitochondria, more so upon exposure to LPS. Mitochondrial reactive oxygen species were higher after LPS (P = 0.03), but not after addition of MCC950. Myotubes had higher glycolytic rates, and mitochondria were more fragmented upon LPS exposure, which was not altered by MCC950 supplementation.Conclusions LPS- induced activation of the NLRP3 inflammasome in cultured myotubes contributes to morphological and metabolic alterations, likely due to its mitochondrial association. Show less
Stel, W. van der; Yang, H.; Le Dévédec, S.E.; Water, B. van de; Beltman, J.B.; Danen, E.H.J. 2022
Cells can adjust their mitochondrial morphology by altering the balance between mitochondrial fission and fusion to adapt to stressful conditions. The connection between a chemical perturbation,... Show moreCells can adjust their mitochondrial morphology by altering the balance between mitochondrial fission and fusion to adapt to stressful conditions. The connection between a chemical perturbation, changes in mitochondrial function, and altered mitochondrial morphology is not well understood. Here, we made use of high-throughput high-content confocal microscopy to assess the effects of distinct classes of oxidative phosphorylation (OXPHOS) complex inhibitors on mitochondrial parameters in a concentration and time resolved manner. Mitochondrial morphology phenotypes were clustered based on machine learning algorithms and mitochondrial integrity patterns were mapped. In parallel, changes in mitochondrial membrane potential (MMP), mitochondrial and cellular ATP levels, and viability were microscopically assessed. We found that inhibition of MMP, mitochondrial ATP production, and oxygen consumption rate (OCR) using sublethal concentrations of complex I and III inhibitors did not trigger mitochondrial fragmentation. Instead, complex V inhibitors that suppressed ATP and OCR but increased MMP provoked a more fragmented mitochondrial morphology. In agreement, complex V but not complex I or III inhibitors triggered proteolytic cleavage of the mitochondrial fusion protein, OPA1. The relation between increased MMP and fragmentation did not extend beyond OXPHOS complex inhibitors: increasing MMP by blocking the mPTP pore did not lead to OPA1 cleavage or mitochondrial fragmentation and the OXPHOS uncoupler FCCP was associated with OPA1 cleavage and MMP reduction. Altogether, our findings connect vital mitochondrial functions and phenotypes in a high-throughput high-content confocal microscopy approach that help understanding of chemical-induced toxicity caused by OXPHOS complex perturbing chemicals. Show less
Many languages are said to possess “gender”, that is, a morphosyntactic system in which nouns induce formal marking on other words beyond the noun itself (adjectives, verbs, etc.). Gorwaa (gow;... Show moreMany languages are said to possess “gender”, that is, a morphosyntactic system in which nouns induce formal marking on other words beyond the noun itself (adjectives, verbs, etc.). Gorwaa (gow; South-Cushitic; Tanzania) possesses a gender system which is interrelated with number in a complex manner. Following the line of reasoning that biological (semantic) sex, grammatical (syntactic) gender, and (morphological) form-class are “interrelated but autonomous domains of linguistic generalization” set out in Harris’(1991) examination of Spanish, and establishing that number and gender are interrelated in a complex manner, this paper considers the morphophonological word-markers of Gorwaa, a language whose nominal morphology is considerably different from that of Spanish. Following a discussion of gender and number in Gorwaa, all word-markers and their associated gender and number values are identified. In addition to being a useful exercise in arranging the empirical data, this paper sheds light on some surprising surface patterns of a little-studied language. Show less