This dissertation examines the Malayic varieties spoken in Kelantan and Terengganu, two Malaysian states located on the northeast coast of the Malay Peninsula. It focuses on three varieties, namely... Show moreThis dissertation examines the Malayic varieties spoken in Kelantan and Terengganu, two Malaysian states located on the northeast coast of the Malay Peninsula. It focuses on three varieties, namely Kelantan Malay, Coastal Terengganu Malay and Inland Terengganu Malay, all belonging to the Malayic subgroup within the Austronesian language family. On the basis of data gathered through linguistic fieldwork undertaken by the author, this dissertation provides the most comprehensive description of these languages to date and offers an analysis of their historical developments.The three Malayic varieties under investigation display notable similarities in their phonological and morphosyntactic patterns, characterised by significant phonological and morphological reduction. Despite these common features, however, it is concluded that these varieties do not form a discrete subgroup within the Malayic languages. The common changes observed in their consonant systems must have followed distinct developments in the vowel systems. Furthermore, the morphological reduction was primarily driven by internal phonological changes, and no clear traces of substrate influences have been found.It is proposed that Inland Terengganu Malay represents a separate variety, distinct from Kelantan Malay and Coastal Terengganu Malay. A two-wave migration pattern is suggested: speakers of Inland Terengganu Malay likely arrived in the region earlier, whereas the speakers of Kelantan Malay and Coastal Terengganu Malay arrived at a later stage. Show less
Several groups within tribe Phyllantheae (Phyllanthaceae) formed, independently, an (obligate) pollination mutualism with Epicephala moths, which originally had been parasitic. In this pollination... Show moreSeveral groups within tribe Phyllantheae (Phyllanthaceae) formed, independently, an (obligate) pollination mutualism with Epicephala moths, which originally had been parasitic. In this pollination system, female moths actively collect pollen from staminate flowers and deposit it on the stigma of pistillate flowers, after which they place at least one egg in or against the ovary. The high pollination rate makes the system beneficial for the plants, whereas the larvae are provided with food (part of the developing seeds) and some protection against predation. Qualitative comparisons are made between non-moth-pollinated lineages, used as outgroups and various, independently moth-pollinated Phyllantheae clades, used as ingroups, thereby looking for parallel developments. The flowers of both sexes of various groups display similar, convergent morphological adaptations to the pollination system, likely to secure the obligate relationship and to improve efficiency. Sepals in both sexes, free or partly to highly connate, are commonly upright and form a narrow tube. The staminate flowers often have united, vertical stamens with the anthers along the androphore or on top of the androphore. Pistillate flowers generally reduce the stigmatic surface, either by making the stigmas shorter or by uniting them into a cone with a small opening at the top for pollen deposition. Less obvious is the reduction of the stigmatic papillae; these are often present in non-moth-pollinated taxa, but absent in the moth-pollinated species. The most diverging, parallel adaptations to moth pollination are currently found in the Palaeotropics, whereas in the Neotropics, some groups continue to also be pollinated by other insect groups and are morphologically less changed. Show less
The Homeric adjectives θοῦρος and θοῦρις (gen. -ιδος) are normally glossed as ‘rushing, impetuous, furious’. While θοῦρις exclusively qualifies feminine nouns, no feminine form of θοῦρος is... Show moreThe Homeric adjectives θοῦρος and θοῦρις (gen. -ιδος) are normally glossed as ‘rushing, impetuous, furious’. While θοῦρις exclusively qualifies feminine nouns, no feminine form of θοῦρος is attested. What was the exact relationship between θοῦρος and θοῦρις? In this paper it is argued that θοῦρις is not the paradigmatic feminine of θοῦρος, but an artificial formation of epic Greek. It arose in the formula θούριδος ἀλκῆς due to the metrical constraints of epic hexameter, and subsequently ousted the original feminine of θοῦρος. In elaborating this scenario, I show that the basic meaning of θοῦρος and θοῦρις is ‘fierce’. Other instances of artificial change of inflection in the Homeric Kunstsprache are discussed, and it is argued that the mechanism underlying their creation is linguistic contamination. Show less
Eggelbusch, M.; Shi, A.D.; Broeksma, B.C.; Vazquez-Cruz, M.; Soares, M.N.; Wit, G.M.J. de; ... ; Wust, R.C.I. 2022
Background Systemic inflammation is associated with skeletal muscle atrophy and metabolic dysfunction. Although the nucleotide-binding oligomerization domain-like receptor family pyrin domain... Show moreBackground Systemic inflammation is associated with skeletal muscle atrophy and metabolic dysfunction. Although the nucleotide-binding oligomerization domain-like receptor family pyrin domain containing 3 (NLRP3) inflammasome contributes to cytokine production in immune cells, its role in skeletal muscle is poorly understood. Here, we studied the link between inflammation, NLRP3, muscle morphology, and metabolism in in vitro cultured C2C12 myotubes, independent of immune cell involvement.Methods Differentiated C2C12 myotubes were treated with lipopolysaccharide (LPS; 0, 10, and 100-200 ng/mL) to induce activation of the NLRP3 inflammasome with and without MCC950, a pharmacological inhibitor of NLRP3-induced IL-1 ss production. We assessed markers of the NLRP3 inflammasome, cell diameter, reactive oxygen species, and mitochondrial function.Results NLRP3 gene expression and protein concentrations increased in a time-dependent and dose-dependent manner. Intracellular IL-1 ss concentration significantly increased (P < 0.0001), but significantly less with MCC950 (P = 0.03), suggestive of moderate activation of the NLRP3 inflammasome in cultured myotubes upon LPS stimulation. LPS suppressed myotube growth after 24 h (P = 0.03), and myotubes remained smaller up to 72 h (P = 0.0009). Exposure of myotubes to IL-1 ss caused similar alterations in cell morphology, and MCC950 mitigated these LPS-induced differences in cell diameter. NLRP3 appeared to co-localize with mitochondria, more so upon exposure to LPS. Mitochondrial reactive oxygen species were higher after LPS (P = 0.03), but not after addition of MCC950. Myotubes had higher glycolytic rates, and mitochondria were more fragmented upon LPS exposure, which was not altered by MCC950 supplementation.Conclusions LPS- induced activation of the NLRP3 inflammasome in cultured myotubes contributes to morphological and metabolic alterations, likely due to its mitochondrial association. Show less
Stel, W. van der; Yang, H.; Le Dévédec, S.E.; Water, B. van de; Beltman, J.B.; Danen, E.H.J. 2022
Cells can adjust their mitochondrial morphology by altering the balance between mitochondrial fission and fusion to adapt to stressful conditions. The connection between a chemical perturbation,... Show moreCells can adjust their mitochondrial morphology by altering the balance between mitochondrial fission and fusion to adapt to stressful conditions. The connection between a chemical perturbation, changes in mitochondrial function, and altered mitochondrial morphology is not well understood. Here, we made use of high-throughput high-content confocal microscopy to assess the effects of distinct classes of oxidative phosphorylation (OXPHOS) complex inhibitors on mitochondrial parameters in a concentration and time resolved manner. Mitochondrial morphology phenotypes were clustered based on machine learning algorithms and mitochondrial integrity patterns were mapped. In parallel, changes in mitochondrial membrane potential (MMP), mitochondrial and cellular ATP levels, and viability were microscopically assessed. We found that inhibition of MMP, mitochondrial ATP production, and oxygen consumption rate (OCR) using sublethal concentrations of complex I and III inhibitors did not trigger mitochondrial fragmentation. Instead, complex V inhibitors that suppressed ATP and OCR but increased MMP provoked a more fragmented mitochondrial morphology. In agreement, complex V but not complex I or III inhibitors triggered proteolytic cleavage of the mitochondrial fusion protein, OPA1. The relation between increased MMP and fragmentation did not extend beyond OXPHOS complex inhibitors: increasing MMP by blocking the mPTP pore did not lead to OPA1 cleavage or mitochondrial fragmentation and the OXPHOS uncoupler FCCP was associated with OPA1 cleavage and MMP reduction. Altogether, our findings connect vital mitochondrial functions and phenotypes in a high-throughput high-content confocal microscopy approach that help understanding of chemical-induced toxicity caused by OXPHOS complex perturbing chemicals. Show less
Tin electrodeposition applications have rapidly evolved in the past 25 years. Usage of tin coatings has advanced from being mainly used for corrosion protection and decorative purposes, to being... Show moreTin electrodeposition applications have rapidly evolved in the past 25 years. Usage of tin coatings has advanced from being mainly used for corrosion protection and decorative purposes, to being used in modern technology such in electronic devices, photovoltaic cells and Li-ion batteries. The new tin coating applications have also come with challenges that require the production of nanostructured deposits, multilayers coatings and composites. Furthermore, the need to reduce energy and source consumptions, and the implementation of more environment-friendly processes, require detailed and fundamental knowledge of the electrodeposition process.The emphasis throughout this thesis is therefore to obtain detailed mechanistic information of tin electrodeposition process.The experimental and theoretical work presented in this thesis attempts to understand the mechanism of tin electrodeposition, and the effect of electrolyte anions and naphthalene-based additives, during the early and subsequent stages of the process. Show less
This book provides a complete analysis of synchronic CV -> VC metathesis in Amarasi, a language of western Timor. Metathesis and unmetathesis realise a paradigm of parallel forms, pairs of... Show moreThis book provides a complete analysis of synchronic CV -> VC metathesis in Amarasi, a language of western Timor. Metathesis and unmetathesis realise a paradigm of parallel forms, pairs of which occur to complement each other throughout the language.Metathesis in Amarasi is superficially associated with a bewildering array of disparate phonological processes including: vowel deletion, consonant deletion, consonant insertion and multiple kinds of vowel assimilation, any of which can (and do) vary by lect in their realisation. By proposing that Amarasi has an obligatory CVCVC foot in which C-slots can be empty, all these phonological processes can be straightforwardly derived from a single rule of metathesis and two associated phonological rules.Three kinds of metathesis can be identified in Amarasi: (i) Before vowel initial enclitics, roots must undergo metathesis, responding to the need to create a phonological boundary between a clitic host and enclitic. Such metathesis is phonologically conditioned. (ii) Metathesis occurs within the syntax to signal attributive modification. Such a metathesised form cannot occur at the end of a phrase and thus requires the presence of an unmetathesised form to complete it syntactically. (iii) In the discourse an unmetathesised form marks an unresolved event or situation. Such an unmetathesised form cannot occur in isolation and requires a metathesised form to achieve resolution.Metathesis in Amarasi is the central linguistic process around which linguistic structures are organised. Amarasi metatheses also reflect fundamental Timorese notions of societal and cosmic organisation. Alongside weaving and other performed activities, metathesis is an important linguistic marker of identity in a region obsessed with similarities and differences between different groups. The complementarity of Amarasi metathesis and unmetathesis within the syntax and within discourse reflects the Timorese division of the world into a series of mutually dependent binary and complementary pairs. As well as being the key which unlocks the structure of the language, metathesis is also a reflection of the structure of Amarasi society and culture. Show less
Morphometric and morphological differences have been used extensively in the past to study domestication of the wolf and the origin of dogs. Certainly before genetic testing was performed, these... Show moreMorphometric and morphological differences have been used extensively in the past to study domestication of the wolf and the origin of dogs. Certainly before genetic testing was performed, these methods were the only means to diverge both groups. But still now, when aDNA cannot be extracted, morphometry and morphology are still important study methods to discern between wolves and dogs.Many of the historically claimed differences were based on studying rather low numbers of Specimens, and on comparisons with genetically isolated groups of dogs or breeds that had anatomical variants which diverge from the mean. This led to several claimed differences that needed rigorous re-evaluated, by investigating larger groups of specimens, both dogs and wolves, and more importantly, and if possible, Pleistocene wolves and the oldest archaeological dogs.We re-evaluated all important morphological and morphometric criteria published in the literature. Most are related to oral, mandibular and skull differences, but also difference in stature was reported. From all criteria we re-tested very few proved to be valuable. And those that show a difference, can often only be used to a limited extend, as only the extremes of these criteria are non-overlapping between groups. Show less
Streptomyces are multicellular, Gram-positive bacteria in the phylum of actinobacteria which produce a high amount of bioactive natural products of which the expression is tightly coordinated with... Show moreStreptomyces are multicellular, Gram-positive bacteria in the phylum of actinobacteria which produce a high amount of bioactive natural products of which the expression is tightly coordinated with the life cycle. This thesis shows the identification of S. roseifaciens, a novel species with an uncommon, verticillate spore morphology and a unique household of SsgA-like proteins. Analyses of the peptidoglycan composition show that S. coelicolor show a pattern of 3-3 cross-linking befitting a tip-growing organism and change in composition between vegetative mycelium and spores. Kitasatosporae carry meso-DAP in the peptidoglycan of vegetative mycelium and LL-DAP in the peptidoglycan of spores. In line with this difference, the peptidoglycan architecture of these two growth stages undergoes such radical changes that they would seem to be from different species. S. coelicolor is naturally vancomycin resistant, but the addition of D-alanine and disruption in a single gene increases vancomycin sensitivity by a thousandfold. A knockout mutant of the alanine racemase, alr, requires exogenous addition of D-alanine. The Alr crystal structure of S. coelicolor and the D-cycloserine producer S. lavendulae were compared as to look for possible mechanisms for D-cycloserine resistance. Show less
Streptomyces are multicellular bacteria that grow as branched filaments and are best known for producing the majority of our antibiotics, many immunosuppressant and anticancer compounds.... Show moreStreptomyces are multicellular bacteria that grow as branched filaments and are best known for producing the majority of our antibiotics, many immunosuppressant and anticancer compounds. Unfortunately their multicellular life style creates many problems for efficient industrial production. In a bioreactor, depending on the environment and the genetics, it can grow quickly as dispersed mycelia or aggregate in slow growing pellets. Either morphology has advantages and disadvantages, which can be product specific. For my thesis I studied the mechanism by which these filaments can aggregate into dense pellets. I found a small gene cluster that produces poly-1,6-N-acetylglucosamine, a bacterial glue which binds neighboring cells and required for pellet formation in S. coelicolor. Subsequently we can use these genes to control the morphology of streptomycetes in a liquid environment, tailoring it for production. My work has given us new insights in the mechanims through which streptomycetes aggregate, but also has the potential to make streptomycetes a more favorable host for industial production. Show less
Streptomyces are Gram-positive, soil dwelling bacteria that raised interest in the last 50 years for their high potential in antibiotic and protein production. Thanks to their saprophytic nature,... Show moreStreptomyces are Gram-positive, soil dwelling bacteria that raised interest in the last 50 years for their high potential in antibiotic and protein production. Thanks to their saprophytic nature, streptomycetes secrete a massive amount of industrial enzymes. They have a relatively low level of endogenous extracellular proteolytic activity when compared to other expression hosts (e.g. Bacillus), they are generally more suited to produce proteins encoded by high G+C actinomycete genes in their native form, coupled to efficient secretion so as to avoid that the proteins end up in inclusion bodies (often a problem when using e.g. E. coli) and making downstream processes easier. Despite their attractive potential, Streptomyces present several constraints which so far limit their application in industry. The first constraint is morphology: by growing as a network of hyphae, they produce dense pellets in liquid cultures that hold Streptomyces back from being one of the first choice cell factories in large scale fermentations. In addition, the limited availability of efficient expression systems for high-level transcription/translation and subsequent secretion is a further bottleneck. This thesis presents the work done to address these issues for the optimization of Streptomyces lividans for future industrial applications and enzyme production. Show less
This book presents a description of Konso, a Cushitic language spoken by about 250,000 speakers in South-West Ethiopia. It presents analyses of the phonology, morphology and syntax of the language.... Show moreThis book presents a description of Konso, a Cushitic language spoken by about 250,000 speakers in South-West Ethiopia. It presents analyses of the phonology, morphology and syntax of the language. Aspects of pragmatics including greetings and leave-taking expressions, interjections and ideophones as well as the link between naming of week days and how these relate to the distribution ofbig markets in the Konso area are discussed. A sample of two texts and a list of singular-plural pairs of nouns with their corresponding gender values is included.The data underlying the analyses are based on the author’s native speaker intuition and fieldwork in Konso area where other native speakers are consulted.Konso phonology is characterised by having a full set of labial, alveolar, palatal and uvular implosives but no ejectives which contrasts with what is observed in geographically and some genetically related languages. The language has a rich morphology as evidenced in its nominal and verbal inflection. The work accounts the intricate link between gender and number marking in nominals,it explicates variation in number- and person-marking in affirmative and negative verb paradigms and presents analyses of nominal and verbal derivation.Various clause-linking strategies and the way these relate to person markingof the subject are examined. Word order in simple as well as complex clausesis discussed.A Grammar of Konso is of interest to specialists in Cushitic and Afroasiatic languages for historical-comparative purposes. It will be a valuable source for typological comparison and for testing theoretical claims Show less
This book presents a description of Sandawe, a Khoisan language spoken by approximately 60 000 speakers in Dodoma Region, Tanzania. The study presents an analysis of the phonology, morphology, and... Show moreThis book presents a description of Sandawe, a Khoisan language spoken by approximately 60 000 speakers in Dodoma Region, Tanzania. The study presents an analysis of the phonology, morphology, and syntax of the language, as well as a sample of four texts. The data for this dissertation were gathered by the author during fieldwork in the area where the language is spoken. The language has a rich phonology, with sets of lateral fricatives and affricates, ejectives, and fifteen clicks. The nominal domain is characterized by the absence of regular number marking. The verbal domain, on the other hand, shows various ways of number marking, which can express participant plurality and pluractionality. The study further provides a comprehensive description of the morphology and semantics of verbal derivation, such as iterative, factitive, causative and middle stems, and verbal case markers that introduce an additional pronominal object. Sandawe has various types of clitics, notably subject/modality markers and mediative clitics, which have a variable position in the clause. A grammar of Sandawe is of relevance to specialists in Khoisan studies as well as to general linguists and typologists interested in number marking, verbal derivation, and clitics. Show less