Biological molecules, especially the proteins, have a special and important function. We study their structure to understand their functions, and further make application, like the medical... Show moreBiological molecules, especially the proteins, have a special and important function. We study their structure to understand their functions, and further make application, like the medical research. The routine method is diffraction, but not work for molecules which cannot grow into crystal and molecules which their crystal are too small. Cryo-EM technique provides another way to solve their structures through their images, it does not need crystals. Meanwhile, electron diffraction can work small crystals (micro- and nano-crystals) after the sample preparation was improved. Hence, we try to build a method that can restore the structure from the crystal’s image. We collect images of protein nano-crystal, and these images were processed to enhance their contrast. The key step is to find the orientations of these images in the procedure of reconstruction, therefore, we create a method that calculates these orientations. Nano-crystals, which cannot be used in diffraction method, then can be used in this method. Show less
In the past decade, advances in structure determination with electron microscopy of organic, beam sensitive, materials have been significant. The newly developed techniques, triggered by new... Show moreIn the past decade, advances in structure determination with electron microscopy of organic, beam sensitive, materials have been significant. The newly developed techniques, triggered by new microscope systems and new cameras, made it possible to acquire 3D structural information from these samples to a resolution which was impossible to achieve before. Knowledge is required to improve structure solution and every aspect of the process involved, from treatment of radiation sensitive materials, sample preparation, TEM imaging and diffraction systems all the way to how data must be interpreted. In this thesis I explained multiple new techniques and methods developed by us, using both new microscopes as well as a new type of detector: Timepix. I describe how these tools can help to overcome (what were) the most important problems and bottlenecks in detection of very low dose electron diffraction. Show less
A surface plasmon is light that is bound to a metal surface. The main merit of a surface plasmon is that is provides confinement below the diffraction limit. In this thesis, we first study the... Show moreA surface plasmon is light that is bound to a metal surface. The main merit of a surface plasmon is that is provides confinement below the diffraction limit. In this thesis, we first study the excitation and scattering of surface plasmons by subwavelength holes in the metal. Thereafter we show that an array of these hole acts as a surface plasmon laser when the surface plasmons are sufficiently amplified using a semiconductor gain medium Show less
In this thesis the prospects of electron diffraction and imaging techniques are discussed to solve the structure of three dimensional nano-crystals. These crystals are beyond the scope of current X... Show moreIn this thesis the prospects of electron diffraction and imaging techniques are discussed to solve the structure of three dimensional nano-crystals. These crystals are beyond the scope of current X-ray techniques. Show less
This thesis describes coupling of light to periodic structures. A material is patterned with a regular pattern on a length scale comparable to the wavelength of light. With these structures, light... Show moreThis thesis describes coupling of light to periodic structures. A material is patterned with a regular pattern on a length scale comparable to the wavelength of light. With these structures, light can be manipulated very precisely. The structures find applications in semiconductor lasers, light emitting diodes (LEDs), photovoltaic cells, and detectors of light. A periodic array of holes in a layer of semiconductor or in a thin metal film causes a coupling between the incident light and light that is trapped inside the layer. This coupling can be studied by measuring the reflection and transmission. The environment has an important role here; e.g. placing glass antennas in the holes can increase the coupling between light and plasmons. A thin, superconducting wire can be used as a detector of light. To increase the surface area, the wire is folded into a meander. The optical properties of this detector are very dependent on the polarization, due to the regular periodic structure of the meander. Moreover, we found that the absorption of a very thin absorbing layer can be almost 100%, when it is illuminated under the right angle, from the substrate. This can be used to increase the efficiency of the detectors. Show less
