This thesis describes the application of a variety of different classical and paramagnetic NMR to different protein system. To this end cytochrome P450cam was assigned using classical techniques... Show moreThis thesis describes the application of a variety of different classical and paramagnetic NMR to different protein system. To this end cytochrome P450cam was assigned using classical techniques and paramagnetic NMR was used to investigate the solution state of this enzyme in complex with its binding partner, putidaredoxin. The solution structure of Avr2 was also solved using classical NMR spectroscopy techniques. Additionally, an algorithm for the assignment of protein nuclei on the basis pseudocontact shifts, PARAssign, is presented. An investigation of the dynamic behaviour of a paramagnetic tag attached to T4 lysozyme using relaxation dispersion is described. Show less
For all organisms populating the Earth, motion is synonymous with life. In the living cell, protein molecules - its most important building blocks and work horses - are constantly on the move.... Show moreFor all organisms populating the Earth, motion is synonymous with life. In the living cell, protein molecules - its most important building blocks and work horses - are constantly on the move. Traditional static models are no longer sufficient for a thorough description of interactions among these biomolecules. Here we present a direct, experimental approach for assessing the dynamics of interactions between proteins, which for a long time has been an exclusive realm of theoretical studies. Show less
