This thesis describes the potential use of these cardiac progenitor-derived extracellular vesicles (EVs) for cardiac repair and focused on translational aspects that could accelerate clinical... Show moreThis thesis describes the potential use of these cardiac progenitor-derived extracellular vesicles (EVs) for cardiac repair and focused on translational aspects that could accelerate clinical implementation of EV-based therapeutics. The first steps have been made in optimizing EV production processes such as EV isolation and storage. Furthermore, this thesis described a potential method for sustained EV release and to prolong therapeutic exposure by using a hydrogel. Ultimately, this could contribute to improved efficacy upon local delivery of EV therapeutics. Together, this thesis potentially contributes to fasten clinical adoption of EV-based therapeutics for patients with heart failure. Show less
The aim of this study is to prepare in situ forming hydrogels based on biocompatible polymers for the controlled release of hydrophobic drug and proteins. In order to load hydrophobic drug to the... Show moreThe aim of this study is to prepare in situ forming hydrogels based on biocompatible polymers for the controlled release of hydrophobic drug and proteins. In order to load hydrophobic drug to the hydrophilic hydrogel matrix, beta-cyclodextrin and human serum albumin was introduced to the hydrogel network respectively and acted as the primary accommodation for those hydrophobic molecules within the hydrogel network. Furthermore, supramolecular crosslinked and covalently crosslinked light sensitive hydrogels were prepared whose potential application for light controlled protein release system has been shown. Show less
