Bantu language (A45). The language has a rich system of 22 non-negative verbal paradigms expressing tense, aspect and mood, and additional negative constructions. The paradigms are built on a TAM... Show moreBantu language (A45). The language has a rich system of 22 non-negative verbal paradigms expressing tense, aspect and mood, and additional negative constructions. The paradigms are built on a TAM word with segmental and tonal properties in association with the choice of which set of subject pronouns to use and which shape of the verb stem to use. Verbs appear in two possible shapes; one of which contains a petrified suffix which is etymologically related to the Bantu *-a(n)g imperfective or pluractional suffix. A crucial property of each verbal paradigm is the position of the full object: for certain paradigms, this position is before the verb contrary to the general pattern in Bantu which tends to be strictly VO for full objects; in other paradigms, the object must occur after the verb; a third group allows both orders. Negative constructions have a clause final clitic. Nyokon is a tonal language and the article discusses the basic tonal rules that are needed for an analysis of the verbal paradigms. The analysis of any sentence in Nyokon requires an understanding of the verbal paradigm and to which verbal paradigm it belongs. Therefore, this article aims at providing the groundwork for further analyses of Nyokon. The emphasis is on the formal characteristics of the verbal paradigms while a substantial analysis of the semantics and functional distinctions of these paradigms awaits the next study, for which the labels used for the various paradigms here only function as first approximations. Show less
The article provides an overview of the formal aspects of the verbal paradigms of Nyokon, a Cameroonian Bantu language (A45). The language has a rich system of 22 non-negative verbal paradigms... Show moreThe article provides an overview of the formal aspects of the verbal paradigms of Nyokon, a Cameroonian Bantu language (A45). The language has a rich system of 22 non-negative verbal paradigms expressing tense, aspect and mood, and additional negative constructions. The paradigms are built on a TAM word with segmental and tonal properties in association with the choice of which set of subject pronouns to use and which shape of the verb stem to use. Verbs appear in two possible shapes; one of which contains a petrified suffix which is etymologically related to the Bantu *-a(n)g imperfective or pluractional suffix. A crucial property of each verbal paradigm is the position of the full object: for certain paradigms, this position is before the verb contrary to the general pattern in Bantu which tends to be strictly VO for full objects; in other paradigms, the object must occur after the verb; a third group allows both orders. Negative constructions have a clause final clitic. Nyokon is a tonal language and the article discusses the basic tonal rules that are needed for an analysis of the verbal paradigms. The analysis of any sentence in Nyokon requires an understanding of the verbal paradigm and to which verbal paradigm it belongs. Therefore, this article aims at providing the groundwork for further analyses of Nyokon. The emphasis is on the formal characteristics of the verbal paradigms while a substantial analysis of the semantics and functional distinctions of these paradigms awaits the next study, for which the labels used for the various paradigms here only function as first approximations Show less
The type-IV secretion system (T4SS) is a machinery able to transfer DNA and proteins between bacteria and in certain cases also to eukaryotic cells. This thesis compared the T4SSs present in... Show moreThe type-IV secretion system (T4SS) is a machinery able to transfer DNA and proteins between bacteria and in certain cases also to eukaryotic cells. This thesis compared the T4SSs present in Agrobacterium tumefaciens and the conjugative plasmid RP4. A. tumefaciens is able to transfer DNA into plant cells and transform them genetically. We discovered that a novel hairpin structure on the terminus of the transferred DNA allows for earlier expression of its genes in the recipient plant cell. Further, we could describe the translocation signal of the relaxase protein TraI of RP4 responsible for DNA processing and transfer via the T4SS. Additionally, it was shown that the T4SS of RP4 is able to translocate proteins from one cell to another without parallel DNA transfer. Furthermore, we were able to create a hybrid relaxase created consisting of parts from A. tumefaciens (relaxase part) and RP4 (translocation signal) that was shown to be translocated via the T4SS of RP4. Show less
The type-IV secretion system (T4SS) is a machinery able to transfer DNA and proteins between bacteria and in certain cases also to eukaryotic cells. This thesis compared the T4SSs present in... Show moreThe type-IV secretion system (T4SS) is a machinery able to transfer DNA and proteins between bacteria and in certain cases also to eukaryotic cells. This thesis compared the T4SSs present in Agrobacterium tumefaciens and the conjugative plasmid RP4. A. tumefaciens is able to transfer DNA into plant cells and transform them genetically. We discovered that a novel hairpin structure on the terminus of the transferred DNA allows for earlier expression of its genes in the recipient plant cell. Further, we could describe the translocation signal of the relaxase protein TraI of RP4 responsible for DNA processing and transfer via the T4SS. Additionally, it was shown that the T4SS of RP4 is able to translocate proteins from one cell to another without parallel DNA transfer. Furthermore, we were able to create a hybrid relaxase created consisting of parts from A. tumefaciens (relaxase part) and RP4 (translocation signal) that was shown to be translocated via the T4SS of RP4. Show less
Wal, S. van der; Kuil, J.; Valentijn, A.R.P.M.; Leeuwen, F.W.B. van 2016
Agrobacterium tumefaciens is a phytopathogen which is known to be the causal agent of crown gall disease in dicotyledonous plants. Virulent Agrobacterium strains are always carrier of a tumor... Show moreAgrobacterium tumefaciens is a phytopathogen which is known to be the causal agent of crown gall disease in dicotyledonous plants. Virulent Agrobacterium strains are always carrier of a tumor-inducing (Ti) plasmid. As a result of the expression of Vir proteins, a section of this plasmid termed the T-DNA is transferred via a type four secretion system (T4SS) as a T-strand to the recipient cell where it can integrate into the genomic DNA of the recipient cell. In this thesis the prerequisites of T-DNA circle formation using yeast as a model were assayed. It was found that the homologous repair (HR) protein Rad52 is of importance to the formation of T-DNA circles. In addition to this, the effects of double strand break (DSB) induction on the efficiency of T-DNA integration via the HR pathway in yeast and the role the nucleosome occupancy of the target locus has on DSB induction were assayed. It was found that a decrease in the nucleosome occupancy of a target locus had no measurable impact on the efficiency of T-DNA integration at this locus. However, the combined translocation of nuclease proteins aimed at the target locus and T-strands during AMT greatly facilitated HR-mediated integration of T-DNA. Show less
