A new type of tripodal squaramide-based supramolecular hydrogels is developed and studied. The mechanical properties of hydrogel with a wide stiffness range that can be easily modulated and also... Show moreA new type of tripodal squaramide-based supramolecular hydrogels is developed and studied. The mechanical properties of hydrogel with a wide stiffness range that can be easily modulated and also special and temporal controlled either by decorating with the activated group to offer additional chemical crosslinks or using hybrid hydrogel by incorporating the second network through light irradiation to overcome the weakness of traditional self-assembled supramolecular materials. Moreover, the designed synthetic hydrogel systems are biocompatible with several cell lines and have the potential to use as a 3D culture substrate. Show less