Michi, Verduijn, as it either provided additional information about perfusion or confirmed the clinical assess-ment. Our pilot study showed a significant decrease of FN in patients undergoing an... Show moreMichi, Verduijn, as it either provided additional information about perfusion or confirmed the clinical assess-ment. Our pilot study showed a significant decrease of FN in patients undergoing an ABR with a DIEP when near-infrared fluorescence imaging was used to assess flap perfusion. This study provides a standardized working protocol for near-infrared fluorescence imaging. In the future, large multicenter studies should focus on the quantification of near-infrared fluorescence imaging. (c) 2021 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Else-vier Ltd. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ ) Show less