Conclusions: FR alpha is a favorable target for fluorescence-guided surgery as EC17 produced a clear fluorescent signal in ovarian and breast cancer tissue. This resulted in resection of ovarian... Show moreConclusions: FR alpha is a favorable target for fluorescence-guided surgery as EC17 produced a clear fluorescent signal in ovarian and breast cancer tissue. This resulted in resection of ovarian cancer lesions that were otherwise not detected. Notwithstanding, autofluorescence caused false-positive lesions in ovarian cancer and difficulty in discriminating breast cancer-specific fluorescence from background signal. Optimization of the 500nm fluorophore, will minimize autofluorescence and further improve intraoperative tumor detection. Show less
Conclusion: This is the first study to show high L1CAM-expression at the infiltrating margin of VSCC's. L1CAM-expressing VSCCs had a significantly worse prognosis compared to L1CAM-negative tumours... Show moreConclusion: This is the first study to show high L1CAM-expression at the infiltrating margin of VSCC's. L1CAM-expressing VSCCs had a significantly worse prognosis compared to L1CAM-negative tumours. The highest expression was observed in spindle-shaped cells, where it might be correlated to their invasive capacity. Show less
Koppers-Lalic, D.; Hackenberg, M.; Menezes, R. de; Misovic, B.; Wachalska, M.; Geldof, A.; ... ; Bijnsdorp, I. 2016
Chronic muscle diseases are highly prevalent in the elderly causing severe mobility limitations, pain and frailty. The intrinsic molecular mechanisms are poorly understood due to multifactorial... Show moreChronic muscle diseases are highly prevalent in the elderly causing severe mobility limitations, pain and frailty. The intrinsic molecular mechanisms are poorly understood due to multifactorial causes, slow progression with age and variations between individuals. Understanding the underlying molecular mechanisms could lead to new treatment options which are currently limited. Shoulder complaints are highly common in the elderly, and therefore, muscles of the shoulder’s rotator cuff could be considered as a model for chronic age-associated muscle degeneration. Diseased shoulder muscles were characterized by muscle atrophy and fatty infiltration compared with unaffected shoulder muscles. We confirmed fatty infiltration using histochemical analysis. Additionally, fibrosis and loss of contractile myosin expression were found in diseased muscles. Most cellular features, including proliferation rate, apoptosis and cell senescence, remained unchanged and genome-wide molecular signatures were predominantly similar between diseased and intact muscles. However, we found down-regulation of a small subset of muscle function genes, and up-regulation of extracellular region genes. Myogenesis was defected in muscle cell culture from diseased muscles but was restored by elevating MyoD levels. We suggest that impaired muscle functionality in a specific environment of thickened extra-cellular matrix is crucial for the development of chronic age-associated muscle degeneration. Show less
Chronic muscle diseases are highly prevalent in the elderly causing severemobility limitations, pain and frailty. The intrinsic molecular mechanisms are poorlyunderstood due to multifactorial... Show moreChronic muscle diseases are highly prevalent in the elderly causing severemobility limitations, pain and frailty. The intrinsic molecular mechanisms are poorlyunderstood due to multifactorial causes, slow progression with age and variationsbetween individuals. Understanding the underlying molecular mechanisms couldlead to new treatment options which are currently limited. Shoulder complaints arehighly common in the elderly, and therefore, muscles of the shoulder’s rotator cuffcould be considered as a model for chronic age-associated muscle degeneration.Diseased shoulder muscles were characterized by muscle atrophy and fatty infiltrationcompared with unaffected shoulder muscles. We confirmed fatty infiltration usinghistochemical analysis. Additionally, fibrosis and loss of contractile myosin expressionwere found in diseased muscles. Most cellular features, including proliferation rate,apoptosis and cell senescence, remained unchanged and genome-wide molecularsignatures were predominantly similar between diseased and intact muscles.However, we found down-regulation of a small subset of muscle function genes,and up-regulation of extracellular region genes. Myogenesis was defected in musclecell culture from diseased muscles but was restored by elevating MyoD levels. Wesuggest that impaired muscle functionality in a specific environment of thickenedextra-cellular matrix is crucial for the development of chronic age-associated muscledegeneration. Show less
