This study reports the sequence specific chemical shifts assignments for 76 residues of the 94 residues containing monomeric unit of the photosynthetic light-harvesting 2 transmembrane protein... Show moreThis study reports the sequence specific chemical shifts assignments for 76 residues of the 94 residues containing monomeric unit of the photosynthetic light-harvesting 2 transmembrane protein complex from Rhodopseudomonas acidophila strain 10050, using Magic Angle Spinning (MAS) NMR in combination with extensive and selective biosynthetic isotope labeling methods. The sequence specific chemical shifts assignment is an essential step for structure determination by MAS NMR. Assignments have been performed on the basis of 2-dimensional proton-driven spin diffusion C-13-C-13 correlation experiments with mixing times of 20 and 500 ms and band selective C-13-C-13 correlation spectroscopy on a series of site-specific biosynthetically labeled samples. The decreased line width and the reduced number of correlation signals of the selectively labeled samples with respect to the uniformly labeled samples enable to resolve the narrowly distributed correlation signals of the backbone carbons and nitrogens involved in the long alpha-helical transmembrane segments. Inter-space correlations between nearby residues and between residues and the labeled BChl a cofactors, provided by the C-13-C-13 correlation experiments using a 500 ms spin diffusion period, are used to arrive at sequence specific chemical shift assignments for many residues in the protein complex. In this way it is demonstrated that MAS NMR methods combined with site-specific biosynthetic isotope labeling can be used for sequence specific assignment of the NMR response of transmembrane proteins. Show less
Partly biosynthetic site-directed isotopically C-13 enriched photosynthetic light-harvesting 2(LH2) complexes have been prepared from Rhodopseudomonas acidophila strain 10050 by using chemically... Show morePartly biosynthetic site-directed isotopically C-13 enriched photosynthetic light-harvesting 2(LH2) complexes have been prepared from Rhodopseudomonas acidophila strain 10050 by using chemically labeled [1,2,3,4-C-13], [1,4-C-13] and [2,3-C-13] succinic acid as a precursor in the growth medium. Two-dimensional proton driven spin diffusion (PDSD) solid state NMR correlation spectroscopy has been used to trace each individual 13C isotope from the labeled succinic acid precursor to its destination into the protein and into the embedded major light-absorbing bacteriochlorophyll cofactors. For both the residues of the protein and for the cofactors distinct labeling patterns have been deduced, for protein complexes prepared from [1,4-C-13]-succinic acid or [2,3-C-13]-succinic labeled media. All residues, except isoleucine and leucine, have been labeled almost homogeneously by the succinic acid precursor. Carbonyl carbons in the protein backbone were labeled by [1,4-C-13]-succinic acid, while the Calpha and Cbeta carbons of the residues were labeled by [2,3-C-13]-succinic acid. Leucine and isoleucine residues were labeled using a uniformly labeled amino acid mixture in the medium. The pattern labeling yields an increase of the resolution and less spectral crowding. The partial labeling technique in combination with conventional solid state NMR methods at ultra high magnetic fields provides an attractive route to resolve chemical shifts for alpha-helical transmembrane protein structures. Show less
Alia, A.; Matysik, J.; Boer, I. de; Gast, P.; Gorkom, H.J. van; Groot, H.J.M. de 2004
The relaxation enhancement caused by paramagnetic copper(II) is used to observe selectively the metal site of copper(I)-amicyanin by one- and two-dimensional NMR spectroscopy. The paramagnetic... Show moreThe relaxation enhancement caused by paramagnetic copper(II) is used to observe selectively the metal site of copper(I)-amicyanin by one- and two-dimensional NMR spectroscopy. The paramagnetic effect is communicated to the diamagnetic protein through the electron self-exchange reaction in partially oxidised samples, and call be used for the selective detection of protons around the metal. Relaxation-selective NMR pulse sequences, like super-WEFT and WEFT-NOESY, are used to achieve the desired selection of the signals. The spectra obtained show well-resolved signals corresponding to protons within a radius of similar to 7 Angstrom from the metal, including almost all protons from the coordinated residues. A significant increase in resolution as well as selection of the most relevant part of the protein (close to the active centre) are the principal advantages of this technique, which can be used to obtain specific information about the metal site in blue copper proteins, to assist in the assignment of their NMR spectra and to determine functional properties like the electron self-exchange rate. Show less
