Depolarization-induced automaticity (DIA) of cardiomyocytes is the property of those cells to generate pacemaker cell-like spontaneous electrical activity when subjected to a depolarizing current.... Show moreDepolarization-induced automaticity (DIA) of cardiomyocytes is the property of those cells to generate pacemaker cell-like spontaneous electrical activity when subjected to a depolarizing current. This property provides a candidate mechanism for generation of pathogenic ectopy in cardiac tissue. The purpose of this study was to determine the biophysical mechanism of DIA in terms of the ion conductance properties of the cardiomyocyte membrane. First, we determined, by use of the conventional whole-cell patch-clamp technique, the membrane conductance and DIA properties of ventricular cardiomyocytes isolated from adult rat heart. Second, we reproduced and analysed DIA properties by using an adapted version of the experimentally based mathematical cardiomyocyte model of Pandit et al. (Biophys J 81:3029-3051 2001, Biophys J 84:832-841 2003) and Padmala and Demir (J Cardiovasc Electrophysiol 14:990-995 2003). DIA in 23 rat cardiomyocytes was a damped membrane potential oscillation with a variable number of action potentials and/or waves, depending on the strength of the depolarizing current and the particular cell. The adapted model was used to reconstruct the DIA properties of a particular cardiomyocyte from its whole-cell voltage-clamp currents. The main currents involved in DIA were an L-type calcium current (I (CaL)) and a slowly activating and inactivating Kv current (I (ss)), with linear (I (B)) and inward rectifier (I (K1)) currents acting as background currents and I (Na) and I (t) as modulators. Essential for DIA is a sufficiently large window current of a slowly inactivating I (CaL) combined with a critically sized repolarizing current I (ss). Slow inactivation of I (ss) makes DIA transient. In conclusion, we established a membrane mechanism of DIA primarily based on I (CaL), I (ss) and inward rectifier properties; this may be helpful in understanding cardiac ectopy and its treatment. Show less
