Cyanobacterial blooms are a global ecological problem that directly threatens human health and crop safety. Cyanobacteria have toxic effects on aquatic microorganisms, which could drive the... Show moreCyanobacterial blooms are a global ecological problem that directly threatens human health and crop safety. Cyanobacteria have toxic effects on aquatic microorganisms, which could drive the selection for resistance genes. The effect of cyanobacterial blooms on the dispersal and abundance of antibiotic-resistance genes (ARGs) of concern to human health remains poorly known. We herein investigated the effect of cyanobacterial blooms on ARG composition in Lake Taihu, China. The numbers and relative abundances of total ARGs increased obviously during a Planktothrix bloom. More pathogenic microorganisms were present during this bloom than during a Planktothrix bloom or during the non-bloom period. Microcosmic experiments using additional aquatic ecosystems (an urban river and Lake West) found that a coculture of Microcystis aeruginosa and Planktothrix agardhii increased the richness of the bacterial community, because its phycosphere provided a richer microniche for bacterial colonization and growth. Antibiotic-resistance bacteria were naturally in a rich position, successfully increasing the momentum for the emergence and spread of ARGs. These results demonstrate that cyanobacterial blooms are a crucial driver of ARG diffusion and enrichment in freshwater, thus providing a reference for the ecology and evolution of ARGs and ARBs and for better assessing and managing water quality. Show less
The zwitterionic Streptococcus pneumoniae serotype 1 polysaccharide (Sp1) is an important anchor point for our immune system to act against streptococcal infections. Antibodies can recognize Sp1... Show moreThe zwitterionic Streptococcus pneumoniae serotype 1 polysaccharide (Sp1) is an important anchor point for our immune system to act against streptococcal infections. Antibodies can recognize Sp1 saccharides, and it has been postulated that Sp1 can elicit a T-cell-dependent immune reaction as it can be presented by MHC-II molecules. To unravel the molecular mode of action of this unique polysaccharide we here describe the chemical synthesis of a set of Sp1 fragments, ranging from 3 to 12 monosaccharides in length. We outline a unique synthetic approach to overcome the major synthetic challenges associated with the complex Sp1 structure and provide a stereoselective route of synthesis for the oligosaccharide backbone as well as a strategy to introduce the carboxylic acid functions. Molecular dynamics (MD) simulations together with NMR spectroscopy studies reveal that the oligosaccharides take up helical structures with the nona- and dodecasaccharide completing a full helical turn. The 3D structure of the oligosaccharides coincides with the topology required for good interaction with anti-Sp1 antibodies, which has been mapped in detail using STD-NMR. Our study has revealed the Sp1 nona- and dodecasaccharides as promising synthetic antigens, displaying all (3D) structural elements required to mimic the natural polysaccharide and required to unravel the molecular mode of action of these unique zwitterionic polysaccharides. Show less
The work in this thesis has been focused on two subjects. The first is the assembly of alginate oligosaccharides and the generation of building blocks for the enzymatic synthesis of alginate,... Show moreThe work in this thesis has been focused on two subjects. The first is the assembly of alginate oligosaccharides and the generation of building blocks for the enzymatic synthesis of alginate, and the second is the total synthesis of large fragments of the zwitterionic SP1 polysaccharide. With these fragments, details about alginate biosynthesis can be obtained through binding studies with biosynthesis enzymes, conjugate vaccines can be generated and binding studies with major histocompatibility complex II molecules can be studied. Show less
Zhang, C.; Hu, M.; Dong, L.; Xiang, P.; Zhang, Q.; Wu, J.; ... ; Shi, S. 2018
Rapid urbanization drives massive construction, which, in return, leads to ever increasing urban metabolism challenges on the provision of raw materials, as well as the disposal of construction and... Show moreRapid urbanization drives massive construction, which, in return, leads to ever increasing urban metabolism challenges on the provision of raw materials, as well as the disposal of construction and demolition waste. Due to its large volume, the transporting and processing of these materials cause considerable greenhouse gas emissions and land use change. With this circumstance, shortening the supply chains of urban construction by efficient recycling of construction and demolition waste becomes a frontier field for the circular transition of cities. It is particularly important in current China, where the concrete recycling is still rare. This paper aims to map the opportunities and potentials of concrete recycling on the mitigation of greenhouse gas emissions and land use change, with an integrated material flow analysis and life cycle assessment for the case study city – Chongqing, China. For the baseline year 2015, four scenarios representing various recycling routes in Chongqing have been explored: (1) improving brick manufacturing; (2) recycling on-site for road base filling; (3) recycling aggregate for prefabricated concrete component and (4) recycling concrete aggregate for structure use. Results highlighted that different technological routes have different potentials to increase recycling rates but all generate co-benefits on greenhouse gases mitigation and land transformation reduction. Recycling of stony construction and demolition waste for high value concrete aggregate has the biggest potential to bring the co-benefits on greenhouse gases mitigation and land use reduction. Besides, on-site recycling for road-base aggregates also presents a high performance, especially on greenhouse gases mitigation in transport. Based on the sensitivity analysis, policy implications were discussed, highlighting the necessity of to develop the recycling routes that substitute primary gravel with aggregates recycled from the stony waste; unlocking the existing recycling capacity and restricting landfilling. Show less
Zhang, Z.; Ke, M.; Qu, Q.; Peijnenburg, W.J.G.M.; Lu, T.; Zhang, Q.; ... ; Qian, H. 2018
