The work in this thesis has been focused on two subjects. The first is the assembly of alginate oligosaccharides and the generation of building blocks for the enzymatic synthesis of alginate,... Show moreThe work in this thesis has been focused on two subjects. The first is the assembly of alginate oligosaccharides and the generation of building blocks for the enzymatic synthesis of alginate, and the second is the total synthesis of large fragments of the zwitterionic SP1 polysaccharide. With these fragments, details about alginate biosynthesis can be obtained through binding studies with biosynthesis enzymes, conjugate vaccines can be generated and binding studies with major histocompatibility complex II molecules can be studied. Show less
Zhang, C.; Hu, M.; Dong, L.; Xiang, P.; Zhang, Q.; Wu, J.; ... ; Shi, S. 2018
Rapid urbanization drives massive construction, which, in return, leads to ever increasing urban metabolism challenges on the provision of raw materials, as well as the disposal of construction and... Show moreRapid urbanization drives massive construction, which, in return, leads to ever increasing urban metabolism challenges on the provision of raw materials, as well as the disposal of construction and demolition waste. Due to its large volume, the transporting and processing of these materials cause considerable greenhouse gas emissions and land use change. With this circumstance, shortening the supply chains of urban construction by efficient recycling of construction and demolition waste becomes a frontier field for the circular transition of cities. It is particularly important in current China, where the concrete recycling is still rare. This paper aims to map the opportunities and potentials of concrete recycling on the mitigation of greenhouse gas emissions and land use change, with an integrated material flow analysis and life cycle assessment for the case study city – Chongqing, China. For the baseline year 2015, four scenarios representing various recycling routes in Chongqing have been explored: (1) improving brick manufacturing; (2) recycling on-site for road base filling; (3) recycling aggregate for prefabricated concrete component and (4) recycling concrete aggregate for structure use. Results highlighted that different technological routes have different potentials to increase recycling rates but all generate co-benefits on greenhouse gases mitigation and land transformation reduction. Recycling of stony construction and demolition waste for high value concrete aggregate has the biggest potential to bring the co-benefits on greenhouse gases mitigation and land use reduction. Besides, on-site recycling for road-base aggregates also presents a high performance, especially on greenhouse gases mitigation in transport. Based on the sensitivity analysis, policy implications were discussed, highlighting the necessity of to develop the recycling routes that substitute primary gravel with aggregates recycled from the stony waste; unlocking the existing recycling capacity and restricting landfilling. Show less
