Dams contribute to water security, energy supply, and flood protection but also fragment habitats of freshwater species. Yet, a global species-level assessment of dam-induced fragmentation is... Show moreDams contribute to water security, energy supply, and flood protection but also fragment habitats of freshwater species. Yet, a global species-level assessment of dam-induced fragmentation is lacking. Here, we assessed the degree of fragmentation of the occurrence ranges of similar to 10,000 lotic fish species worldwide due to similar to 40,000 existing large dams and similar to 3,700 additional future large hydropower dams. Per river basin, we quantified a connectivity index (CI) for each fish species by combining its occurrence range with a high-resolution hydrography and the locations of the dams. Ranges of nondiadromous fish species were more fragmented (less connected) (CI = 73 +/- 28%; mean +/- SD) than ranges of di-adromous species (CI = 86 +/- 19%). Current levels of fragmentation were highest in the United States, Europe, South Africa, India, and China. Increases in fragmentation due to future dams were especially high in the tropics, with declines in CI of similar to 20 to 40 percentage points on average across the species in the Amazon, Niger, Congo, Salween, and Mekong basins. Our assessment can guide river management at multiple scales and in various domains, including strategic hydropower planning, identification of species and basins at risk, and prioritization of restoration measures, such as dam removal and construction of fish bypasses. Show less