Carnobacterium divergens is frequently isolated from natural environments and is a predominant species found in refrigerated foods, particularly meat, seafood, and dairy. While there is substantial... Show moreCarnobacterium divergens is frequently isolated from natural environments and is a predominant species found in refrigerated foods, particularly meat, seafood, and dairy. While there is substantial interest in using C. divergens as biopreservatives and/or probiotics, some strains are known to be fish pathogens, and the uncontrolled growth of C. divergens has been associated with food spoilage. Bacteriophages offer a selective approach to identify and control the growth of bacteria; however, to date, few phages targeting C. divergens have been reported. In this study, we characterize bacteriophage cd2, which we recently isolated from minced beef. A detailed host range study reveals that phage cd2 infects certain phylogenetic groups of C. divergens. This phage has a latent period of 60 min and a burst size of ~28 PFU/infected cell. The phage was found to be acid and heat sensitive, with a complete loss of phage activity when stored at pH 2 or heated to 60°C. Electron microscopy shows that phage cd2 is a siphophage, and while it shares the B3 morphotype with a unique cluster of Listeria and Enterococcus phages, a comparison of genomes reveals that phage cd2 comprises a new genus of phage, which we have termed as Carnodivirus. Show less
Decarbonizing the building stock is critical for realizing the climate-neutral target for the Netherlands. This thesis uses multi-source data, mainly including GIS data of building footprints and... Show moreDecarbonizing the building stock is critical for realizing the climate-neutral target for the Netherlands. This thesis uses multi-source data, mainly including GIS data of building footprints and the archetypes representative of Dutch residential buildings, to develop a series of bottom-up building stock models to track future material stock and flows, energy demand, electricity generation, and GHG emissions. Results demonstrate the great potential for decarbonizing the Dutch residential building sector, while decarbonization strategies should be effectively and extensively implemented. Saving space heating energy consumption is the most direct way to reduce annual GHG emissions. Considering that most existing residential buildings will still be in use in 2050, renovating them with high energy performance standards is required. Despite the great potential of renovation, it alone is not enough to realize the climate-neutral target in the residential building stock because the upstream fossil fuel-based energy systems still emit large amounts of GHG. Replacing fossil fuels with renewable energy sources is a critical path, mainly involving onsite natural gas combustion for space heating and offsite natural gas and coal combustion for electricity and heat (in heat networks) generation. Urban mining cannot contribute to as much GHG emission reduction as energy transition strategies, though, should still be implemented as it can reduce the primary material consumption and CDW landfill. In addition to the technical aspects considered in this thesis, it is also necessary to develop feasible policies in terms of socioeconomic aspects to guarantee the effective and quick deployment of these technical strategies. Show less
The building sector plays a key role in energy transition and carbon reduction while capturing the dynamic characteristics (e.g. materials, energy performance, and environmental impact) of building... Show moreThe building sector plays a key role in energy transition and carbon reduction while capturing the dynamic characteristics (e.g. materials, energy performance, and environmental impact) of building stock is a great challenge during the gradual process. This study presents a bottom-up dynamic building stock model that links dynamic material flow analysis with building energy modeling. The environmental impact of material and energy requirements is assessed by considering future electricity mix. The model is applied to evaluate the pathways to the climate-neutral energy supply of residential building stock in the Netherlands by 2050. Results show that space heating demand decreases by about 2/3 by 2050, while the energy for hot water increases to 92% of space heating demand. 80% of public grid electricity for appliances and lighting can be potentially substituted if rooftop photovoltaic (PV) systems are installed on 50% of renovated buildings and all the new buildings. Greenhouse gas (GHG) emissions of operational energy are reduced by approximately 60-90%, depending on the electricity mix. Annual GHG emissions from material production are not as important as those related to operational energy. Insulation materials account for a large proportion of the carbon footprint of material production. The model has a high spatial and temporal resolution and can be linked with local energy source availability (e.g. buildings or neighborhoods) to provide more accurate support for policymaking. Show less
Yang, X.; Hu, M.; Zhang, C.; Steubing, B.R.P. 2022
The construction sector is the biggest driver of resource consumption and waste generation in Europe. The European Union (EU) is making efforts to move from its traditional linear resource and... Show moreThe construction sector is the biggest driver of resource consumption and waste generation in Europe. The European Union (EU) is making efforts to move from its traditional linear resource and waste management system in the construction sector to a level of high circularity. Based on the theory of circular economy, a new paradigm called waste hierarchy was introduced in the EU Waste Framework Directive. This work uses the framework of the waste hierarchy to analyze the practice of construction and demolition waste (CDW) management in Europe. We explore the evolution of the waste hierarchy in Europe and how it compares with the circular economy. Then, based on the framework, we analyze the performance of CDW management in each EU member state. Innovative treatment methods of CDW, focusing on waste concrete, is investigated. This brings insight into optimizing and upgrading the CDW management in light of advanced technologies and steering the pathway for transitioning the EU towards a circular society. Show less
Lin, J.; Yang, X.; Kosters, W.A.; Xu, T.; Jia, Y.; Wang, S.; ... ; Ye, K. 2022
Buildings have become a major concern because of their high energy use and carbon emissions. Thus, a material-efficient prefabricated concrete element (PCE) system was developed to incorporate... Show moreBuildings have become a major concern because of their high energy use and carbon emissions. Thus, a material-efficient prefabricated concrete element (PCE) system was developed to incorporate construction and demolition waste as feedstock for residential building energy renovation by over-cladding the walls of old buildings. By conducting life cycle assessment and life cycle costing using the payback approach, this study aims to explore the life cycle performance of energy conservation, carbon mitigation, and cost reduction of the PCE system in three European member states: Spain, the Netherlands, and Sweden. The results show that the energy payback periods for Spain, the Netherlands, and Sweden were 20.45 years, 17.60 years, 19.95 years, respectively, and the carbon payback periods were 23.33 years, 16.78 years, and 8.58 years, respectively. However, the financial payback periods were less likely to be achieved within the building lifetime, revealing that only the Swedish case achieved a payback period within 100 years (83.59 years). Thus, circularity solutions were considered to shorten the PCE payback periods. Using secondary materials in PCE fabrication only slightly reduced the payback period. However, reusing the PCE considerably reduced the energy and carbon payback periods to less than 6 years and 11 years, respectively in all three cases. Regarding cost, reusing the PCE shortened the Swedish payback period to 29.30 years, while the Dutch and Spanish cases achieved investment payback at 42.97 years and 85.68 years, respectively. The results can be extrapolated to support the design of sustainable building elements for energy renovation in Europe. Show less
Zhang, C.; Hu, M.; Sprecher, B.; Yang, X.; Zhong, X.; Li, C.; Tukker, A. 2021
DOM* can not only be regarded as a "controller" of other reactive intermediates, but also effectively promote the photodegradation of bezafibrate, which is classified as a persistent organic... Show moreDOM* can not only be regarded as a "controller" of other reactive intermediates, but also effectively promote the photodegradation of bezafibrate, which is classified as a persistent organic contaminant. This study gives deep insights into effects of UV/chlorine on the photophysical and photochemical properties of DOM, and is helpful for understanding the dynamic roles of DOM in the photodegradation of micropollutants. Show less
Segmentation of medical images, particularly late gadolinium-enhanced magnetic resonance imaging (LGE-MRI) used for visualizing diseased atrial structures, is a crucial first step for ablation... Show moreSegmentation of medical images, particularly late gadolinium-enhanced magnetic resonance imaging (LGE-MRI) used for visualizing diseased atrial structures, is a crucial first step for ablation treatment of atrial fibrillation. However, direct segmentation of LGE-MRIs is challenging due to the varying intensities caused by contrast agents. Since most clinical studies have relied on manual, labor-intensive approaches, automatic methods are of high interest, particularly optimized machine learning approaches. To address this, we organized the 2018 Left Atrium Segmentation Challenge using 154 3D LGE-MRIs, currently the world's largest atrial LGE-MRI dataset, and associated labels of the left atrium segmented by three medical experts, ultimately attracting the participation of 27 international teams. In this paper, extensive analysis of the submitted algorithms using technical and biological metrics was performed by undergoing subgroup analysis and conducting hyper-parameter analysis, offering an overall picture of the major design choices of convolutional neural networks (CNNs) and practical considerations for achieving state-of-the-art left atrium segmentation. Results show that the top method achieved a Dice score of 93.2% and a mean surface to surface distance of 0.7 mm, significantly outperforming prior state-of-the-art. Particularly, our analysis demonstrated that double sequentially used CNNs, in which a first CNN is used for automatic region-of-interest localization and a subsequent CNN is used for refined regional segmentation, achieved superior results than traditional methods and machine learning approaches containing single CNNs. This large-scale benchmarking study makes a significant step towards much-improved segmentation methods for atrial LGE-MRIs, and will serve as an important benchmark for evaluating and comparing the future works in the field. Furthermore, the findings from this study can potentially be extended to other imaging datasets and modalities, having an impact on the wider medical imaging community. (C) 2020 Elsevier B.V. All rights reserved. Show less
Cyanobacterial blooms are a global ecological problem that directly threatens human health and crop safety. Cyanobacteria have toxic effects on aquatic microorganisms, which could drive the... Show moreCyanobacterial blooms are a global ecological problem that directly threatens human health and crop safety. Cyanobacteria have toxic effects on aquatic microorganisms, which could drive the selection for resistance genes. The effect of cyanobacterial blooms on the dispersal and abundance of antibiotic-resistance genes (ARGs) of concern to human health remains poorly known. We herein investigated the effect of cyanobacterial blooms on ARG composition in Lake Taihu, China. The numbers and relative abundances of total ARGs increased obviously during a Planktothrix bloom. More pathogenic microorganisms were present during this bloom than during a Planktothrix bloom or during the non-bloom period. Microcosmic experiments using additional aquatic ecosystems (an urban river and Lake West) found that a coculture of Microcystis aeruginosa and Planktothrix agardhii increased the richness of the bacterial community, because its phycosphere provided a richer microniche for bacterial colonization and growth. Antibiotic-resistance bacteria were naturally in a rich position, successfully increasing the momentum for the emergence and spread of ARGs. These results demonstrate that cyanobacterial blooms are a crucial driver of ARG diffusion and enrichment in freshwater, thus providing a reference for the ecology and evolution of ARGs and ARBs and for better assessing and managing water quality. Show less
Zhang, C.; Hu, M.; Laclau, B.; Garnesson, T.; Yang, X.; Li, C.; Tukker, A. 2020
Around 35% of the buildings in Europe are over 50 years old and almost 75% of the building stock is energy-inefficient. A European project VEEP is developing an innovative prefabricated concrete... Show moreAround 35% of the buildings in Europe are over 50 years old and almost 75% of the building stock is energy-inefficient. A European project VEEP is developing an innovative prefabricated concrete element (PCE) system to improve the thermal performance of new buildings (PCE1) and old buildings (PCE2). This study focused on retrofitting of old buildings via over-cladding of the building envelope with PCE2. This study aims to from a building owner/consumer’s perspective to explore the life cycle economic performance of the PCE2 system at an early stage and associated cost optimization strategies under the European context. This study tries to answer four questions: 1) whether the use of the PCE2 leads to an economic advantage over a specific life cycle of an existing building. 2) what is the biggest cost stressor in the life cycle of a PCE2? 3) the potential route for further cost optimization. and 4) how would the discount rate affect the life cycle costs, especially when Europe has entered a negative rate age? A typical apartment building in the Netherlands is selected as the case study for dynamic thermal simulation, in which the heating and cooling energy demands before and after refurbishment with PCE2 will be evaluated. By employing environmental life cycle costing (LCC), the life cycle costs over 40 years and associated strategy for cost optimization will be investigated. This research not only unveils meaningful financial implications on resource-efficient building energy retrofitting in Europe but also provides insight on methodological dilemmas within the application of LCC. Show less
Zhang, C.; Hu, M.; Yang, X.; Miranda Xicotencatl, B.; Sprecher, B.; Di Maio, F.; ... ; Tukker, A. 2020
Urban mining from construction and demolition waste (CDW) is highly relevant for the circular economy ambitions of the European Union (EU). Given the large volumes involved, end-of-life (EoL)... Show moreUrban mining from construction and demolition waste (CDW) is highly relevant for the circular economy ambitions of the European Union (EU). Given the large volumes involved, end-of-life (EoL) concrete is identified as one of the priority streams for CDW recycling in most EU countries, but it is currently largely downcycled or even landfilled. The European projects C2CA and VEEP have proposed several cost-effective technologies to recover EoL concrete for new concrete manufacturing. To understand the potential effects of large-scale implementation of those recycling technologies on the circular construction, this study deployed static material flow analysis (MFA) for a set of EoL concrete management scenarios in the Netherlands constructed by considering the development factors in two, technological and temporal dimensions. On the technological dimension, three treatment systems for EoL concrete management, namely: business-as-usual treatment, C2CA technological system and VEEP technological system were investigated. On the temporal dimension, 2015 was selected as the reference year, representing the current situation, and 2025 as the future year for the prospective analysis. The results show that the development of cost-effective technologies has the potential to improve the share of recycling (as opposed to downcycling) in the Netherlands from around 5% in 2015 up to 22%~32% in 2025. From the academic aspect, the presented work illustrates how the temporal dimension can be included in the static MFA study to explore the potential effects in the future. Show less
Cervical motion preservation prostheses are considered a developing technology, with widespread clinical use beginning in the early 2000s. They are developed to reduce adjacent segment degeneration... Show moreCervical motion preservation prostheses are considered a developing technology, with widespread clinical use beginning in the early 2000s. They are developed to reduce adjacent segment degeneration (ASD) in the postsurgical follow-up by maintaining range of motion (ROM). However, it is still a controversial issue. The main objective of this thesis was to uncover the relationship between preserved motion and radiological ASD in patients with single-level cervical radiculopathy. Other factors which may be correlated to ASD were studied as well. Show less