Background The benefits of surgery for symptomatic spinal metastases have been demonstrated, largely based on series of patients undergoing debulking and instrumentation operations. However, as... Show moreBackground The benefits of surgery for symptomatic spinal metastases have been demonstrated, largely based on series of patients undergoing debulking and instrumentation operations. However, as cancer treatments improve and overall survival lengths increase, the incidence of recurrent spinal cord compression after debulking may increase. The aim of the current paper is to document the postoperative evolution of neurological function, pain, and quality of life following debulking and instrumentation in the Global Spine Tumor Study Group (GSTSG) database. Methods The GSTSG database is a prospective multicenter data repository of consecutive patients that underwent surgery for a symptomatic spinal metastasis. For the present analysis, patients were selected from the database that underwent decompressive debulking surgery with instrumentation. Preoperative tumor type, Tomita and Tokuhashi scores, EQ-5D, Frankel, Karnofsky, and postoperative complications, survival, EQ-5D, Frankel, Karnofsky, and pain numeric rating scores (NRS) at 3, 6, 12, and 24 months were analyzed. Results A total of 914 patients underwent decompressive debulking surgery with instrumentation and had documented follow-up until death or until 2 years post surgery. Median preoperative Karnofsky performance index was 70. A total of 656 patients (71.8%) had visceral metastases and 490 (53.6%) had extraspinal bone metastases. Tomita scores were evenly distributed above (49.1%) and below or equal to 5 (50.9%), and Tokuhashi scores almost evenly distributed below or equal to 8 (46.3%) and above 8 (53.7%). Overall, 12-month survival after surgery was 56.3%. The surgery resulted in EQ-5D health status improvement and NRS pain reduction that was maintained throughout follow-up. Frankel scores improved at first follow-up in 25.0% of patients, but by 12 months neurological deterioration was observed in 18.8%. Conclusion We found that palliative debulking and instrumentation surgeries were performed throughout all Tomita and Tokuhashi categories. These surgeries reduced pain scores and improved quality of life up to 2 years after surgery. After initial improvement, a proportion of patients experienced neurological deterioration by 1 year, but the majority of patients remained stable. Show less
Jia, Y.; Sun, M.; Shi, Y.; Zhu, Z.; Wijk, E. van; Wijk, R. van; ... ; Wang, M. 2020
The prevalence of type 2 diabetes mellitus (T2DM) is increasing rapidly worldwide. Because of the limited success of generic interventions, the focus of the disease study has shifted toward... Show moreThe prevalence of type 2 diabetes mellitus (T2DM) is increasing rapidly worldwide. Because of the limited success of generic interventions, the focus of the disease study has shifted toward personalized strategies, particularly in the early stages of the disease. Traditional Chinese medicine (TCM) is based on a systems view combined with personalized strategies and has improved our knowledge of personalized diagnostics. From a systems biology perspective, the understanding of personalized diagnostics can be improved to yield a biochemical basis for such strategies; for example, metabolomics can be used in combination with other system-based diagnostic methods such as ultra-weak photon emission (UPE). In this study, we investigated the feasibility of using plasma metabolomics obtained from 44 pre-T2DM subjects to stratify the following TCM-based subtypes: Qi-Yin deficiency, Qi-Yin deficiency with dampness, and Qi-Yin deficiency with stagnation. We studied the relationship between plasma metabolomics and UPE with respect to TCM-based subtyping in order to obtain biochemical information for further interpreting disease subtypes. Principal component analysis of plasma metabolites revealed differences among the TCM-based pre-T2DM subtypes. Relatively high levels of lipids (e.g., cholesterol esters and triglycerides) were important discriminators of two of the three subtypes and may be associated with a higher risk of cardiovascular disease. Plasma metabolomics data indicate that the lipid profile is an essential component captured by UPE with respect to stratifying subtypes of T2DM. The results suggest that metabolic differences exist among different TCM-based subtypes of pre-T2DM, and profiling plasma metabolites can be used to discriminate among these subtypes. Plasma metabolomics thus provides biochemical insights into system-based UPE measurements. (C) 2019 THE AUTHORS. Published by Elsevier LTD on behalf of Chinese Academy of Engineering and Higher Education Press Limited Company. Show less
Plant–soil feedbacks (PSFs) involve changes to the soil wrought by plants, which change biotic and abiotic properties of the soil, affecting plants that grow in the soil at a later time. The... Show morePlant–soil feedbacks (PSFs) involve changes to the soil wrought by plants, which change biotic and abiotic properties of the soil, affecting plants that grow in the soil at a later time. The importance of PSFs for understanding ecosystem functioning has been the focus of much recent research, for example, in predicting the consequences for agricultural production, biodiversity conservation, and plant population dynamics. Here, we describe an experiment designed to test PSFs left by plants with contrasting traits under field conditions. This is one of the first, large-scale field experiments of its kind. We removed the existent plant community and replaced it with target plant communities that conditioned the soil. These communities consisted of contrasting proportions of grass and forb cover and consisted of either fast- or slow-growing plants, in accordance with the plant economics spectrum. We chose this well-established paradigm because plants on opposite ends of this spectrum have developed contrasting strategies to cope with environmental conditions. This means they differ in their feedbacks with soil abiotic and biotic factors. The experimental procedure was repeated in two successive years in two different subplots in order to investigate temporal effects on soils that were conditioned by the same plant community. Our treatments were successful in creating plant communities that differed in their total percentage cover based on temporal conditioning, percentage of grasses versus forbs, and percentage of fast- versus slow-growing plants. As a result, we expect that the influence of these different plant communities will lead to different PSFs. The unique and novel design of this experiment allows us to simultaneously test for the impacts of temporal effects, plant community composition and plant growth strategy on PSFs. Here, we describe the experimental design and demonstrate why this effective design is ideal to advance our understanding of PSFs in the field. Show less
Grammatical gender agreement has been well addressed in language comprehension but less so in language production. The present article discusses the arguments derived from the most prominent... Show moreGrammatical gender agreement has been well addressed in language comprehension but less so in language production. The present article discusses the arguments derived from the most prominent language production models on the representation and processing of the grammatical gender of nouns in language production and then reviews recent empirical studies that provide some answers to these arguments. Show less
Gu, C.G.; Yang, H.J.; Wang, M.; Rohling, J.H.T. 2019
The circadian rhythms in mammals, that are regulated by the suprachiasmatic nucleus (SCN) of the brain, have been observed even in the absence of a light-dark cycle. The SCN is composed of about 10... Show moreThe circadian rhythms in mammals, that are regulated by the suprachiasmatic nucleus (SCN) of the brain, have been observed even in the absence of a light-dark cycle. The SCN is composed of about 10 000 autonomous neuronal oscillators, which are heterogenous in many oscillatory properties, including the heterogeneity in relaxation rates. Although the relaxation rate affects the entrainability of the SCN as a whole, not much is known about the reasons why the heterogeneity in relaxation rate exists. In the present study, based on a Poincare model, we examine whether the heterogeneity in the relaxation rate affects the synchronization of the SCN neuronal oscillators under constant darkness. Both our simulations and theoretical results show that the heterogeneity improves the synchronization. Our findings provide an alternative explanation for the existence of the heterogeneity in the SCN neurons and shed light on the effect of neuronal heterogeneity on the collective behavior of the SCN neurons. Published under license by AIP Publishing. Show less
Plant–soil feedbacks (PSFs) involve changes to the soil wrought by plants, which change biotic and abiotic properties of the soil, affecting plants that grow in the soil at a later time. The... Show morePlant–soil feedbacks (PSFs) involve changes to the soil wrought by plants, which change biotic and abiotic properties of the soil, affecting plants that grow in the soil at a later time. The importance of PSFs for understanding ecosystem functioning has been the focus of much recent research, for example, in predicting the consequences for agricultural production, biodiversity conservation, and plant population dynamics. Here, we describe an experiment designed to test PSFs left by plants with contrasting traits under field conditions. This is one of the first, large-scale field experiments of its kind. We removed the existent plant community and replaced it with target plant communities that conditioned the soil. These communities consisted of contrasting proportions of grass and forb cover and consisted of either fast- or slow-growing plants, in accordance with the plant economics spectrum. We chose this well-established paradigm because plants on opposite ends of this spectrum have developed contrasting strategies to cope with environmental conditions. This means they differ in their feedbacks with soil abiotic and biotic factors. The experimental procedure was repeated in two successive years in two different subplots in order to investigate temporal effects on soils that were conditioned by the same plant community. Our treatments were successful in creating plant communities that differed in their total percentage cover based on temporal conditioning, percentage of grasses versus forbs, and percentage of fast- versus slow-growing plants. As a result, we expect that the influence of these different plant communities will lead to different PSFs. The unique and novel design of this experiment allows us to simultaneously test for the impacts of temporal effects, plant community composition and plant growth strategy on PSFs. Here, we describe the experimental design and demonstrate why this effective design is ideal to advance our understanding of PSFs in the field. Show less
The homeodomain-leucine zipper (HD-Zip) transcription factor family plays vital roles in plant development and morphogenesis as well as responses to biotic and abiotic stresses. In barley, a... Show moreThe homeodomain-leucine zipper (HD-Zip) transcription factor family plays vital roles in plant development and morphogenesis as well as responses to biotic and abiotic stresses. In barley, a recessive mutation in Vrs1 (HvHox1) changes two-rowed barley to six-rowed barley, which improves yield considerably. The Vrs1 gene encodes an HD-Zip subfamily I transcription factor. Phylogenetic analysis has shown that the rice HD-Zip I genes Oshox12 and Oshox14 are the closest homologues of Vrs1. Here, we show that Oshox12 and Oshox14 are ubiquitously expressed with higher levels in developing panicles. Trans-activation assays in yeast and rice protoplasts demonstrated that Oshox12 and Oshox14 can bind to a specific DNA sequence, AH1 (CAAT(A/T)ATTG), and activate reporter gene expression. Overexpression of Oshox12 and Oshox14 in rice resulted in reduced panicle length and a dwarf phenotype. In addition, Oshox14 overexpression lines showed a deficiency in panicle exsertion. Our findings suggest that Oshox12 and Oshox14 may be involved in the regulation of panicle development. This study provides a significant advancement in understanding the functions of HD-Zip transcription factors in rice. Show less
Choi, D.; Ricciardi, F.; Arts, M.; Buchowski, J.M.; Bunger, C.; Chung, C.K.; ... ; Crockard, A. 2018
Ginseng Radix (Panax ginseng) is one of the most commonly used herbs worldwide for the treatment of inflammation-related diseases among others, supported by ancient historical records. Throughout... Show moreGinseng Radix (Panax ginseng) is one of the most commonly used herbs worldwide for the treatment of inflammation-related diseases among others, supported by ancient historical records. Throughout this long history, the large-scale cultivation of ginseng created an increasing demand for long-term storage of the harvested plant material, accelerating the development of post-harvesting procedures. Dried white ginseng and processed (steamed) red ginseng are the products of the two most common traditional post-harvest processes. Although there are a significant number of reports on practice-based therapeutic applications of ginseng, science-based evidence is needed to support these uses. Using a reverse pharmacology approach in conjunction with high-throughput techniques and animal models may offer clear, simple paths for the elucidation of the mechanisms of activity of herbal medicines. Moreover, it could provide a new and more efficient method for the discovery of potential drug candidates. From this perspective, the different chemical compositions of white ginseng and red ginseng could very likely result in different interactions with signaling pathways of diverse biological responses. This paper provides an overview of white ginseng and red ginseng, mainly focusing on their chemical profile and immunomodulation activities. Synergistic effects of ginseng herbal drugs with combinations of other traditional herbal drugs or with synthetic drugs were reviewed. The use of the zebrafish model for bioactivity testing greatly improves the prospects for future ginseng research. Show less
Wright, E.; Ricciardi, F.; Arts, M.; Buchowski, J.M.; Chung, C.K.; Coppes, M.; ... ; Choi, D. 2018
To produce a word, speakers need to retrieve the lexico-syntactic representation of the word and encode the phonological form for articulation. It is not precisely known yet if a word's syntactic... Show moreTo produce a word, speakers need to retrieve the lexico-syntactic representation of the word and encode the phonological form for articulation. It is not precisely known yet if a word's syntactic features (e.g., number, gender, etc.) are automatically activated and selected in bare noun production. Cubelli, Lotto, Paolieri, Girelli, and Job (2005) proposed that only in languages that have a complex morphological structure (e.g., Italian), the selection of grammatical gender is required. In languages with a relatively simpler morphological structure, the selection of grammatical gender is by-passed. Here, we investigated this issue further by employing a language with an extremely simple morphological structure, i.e., Mandarin Chinese. Using the picture-word interference paradigm, we manipulated the congruency of the lexico-syntactic classifier feature (comparable to grammatical gender) between the target picture and the superimposed distractor word. We measured participants' naming latencies and their electroencephalogram (EEG). As a result, relative to the classifier-congruent condition, classifier incongruency elicited a stronger N400 effect in the ERP analyses, suggesting the automatic activation of lexico-syntactic features in bare noun production. However, classifier congruency did not affect naming latencies, suggesting that the lexico-syntactic feature is not selected in bare noun naming when it is irrelevant for production. Show less
The Chinese medicine Qiliqiangxin (QL) has been shown to have a protective role in heart failure. Here, we explore the underlying working mechanism of the key therapeutic component in QL using a... Show moreThe Chinese medicine Qiliqiangxin (QL) has been shown to have a protective role in heart failure. Here, we explore the underlying working mechanism of the key therapeutic component in QL using a rat model of heart failure. Heart failure after myocardial infarction was induced surgically and confirmed using echocardiography; a separate group of rats underwent sham surgery. The rats with heart failure were randomly assigned to receive QL, the angiotensin-converting enzyme inhibitor benazepril, or placebo groups. Blood samples were collected from the rats at four time points for up to 8 weeks and used for biochemical analysis and mass spectrometry‒based metabolomics profiling. In total, we measured nine well-known biochemical parameters of heart failure and 147 metabolites. In the rats with heart failure, QL significantly improved these biochemical parameters and metabolomics profiles, significantly increasing the cardioprotective parameter angiopoietin-like 4 and significantly lowering inflammation-related oxylipins and lysophosphatidic acids compared to benazepril. Mechanistically, QL may improve outcome in heart failure by controlling inflammatory process and cardiac hypertrophy. Clinical studies should be designed in order to investigate these putative mechanisms in patients. Show less
