Chimeric rodent malaria parasites with the endogenous circumsporozoite protein (csp) gene replaced with csp from the human parasites Plasmodium falciparum (Pf) and P. vivax (Pv) are used in... Show moreChimeric rodent malaria parasites with the endogenous circumsporozoite protein (csp) gene replaced with csp from the human parasites Plasmodium falciparum (Pf) and P. vivax (Pv) are used in preclinical evaluation of CSP vaccines. Chimeric rodent parasites expressing PfCSP have also been assessed as whole sporozoite (WSP) vaccines. Comparable chimeric P. falciparum parasites expressing CSP of P. vivax could be used both for clinical evaluation of vaccines targeting PvCSP in controlled human P. falciparum infections and in WSP vaccines targeting P. vivax and P. falciparum. We generated chimeric P. falciparum parasites expressing both PfCSP and PvCSP. These Pf-PvCSP parasites produced sporozoite comparable to wild type P. falciparum parasites and expressed PfCSP and PvCSP on the sporozoite surface. Pf-PvCSP sporozoites infected human hepatocytes and induced antibodies to the repeats of both PfCSP and PvCSP after immunization of mice. These results support the use of Pf-PvCSP sporozoites in studies optimizing vaccines targeting PvCSP. Show less
Kennedy, G.M.; Ginski, C.; Kenworthy, M.A.; Benisty, M.; Henning, T.; Holstein, R.G. van; ... ; Sturm, A. 2020
RZ Psc is a young Sun-like star with a bright and warm infrared excess that is occasionally dimmed significantly by circumstellar dust structures. Optical depth arguments suggest that the dimming... Show moreRZ Psc is a young Sun-like star with a bright and warm infrared excess that is occasionally dimmed significantly by circumstellar dust structures. Optical depth arguments suggest that the dimming events do not probe a typical sightline through the circumstellar dust, and are instead caused by structures that appear above an optically thick mid-plane. This system may therefore be similar to systems where an outer disc is shadowed by material closer to the star. Here, we report the discovery that RZ Psc hosts a 0.12 M circle dot companion at a projected separation of 23 au. We conclude that the disc must orbit the primary star. While we do not detect orbital motion, comparison of the angle of linear polarization of the primary with the companion's on-sky position angle provides circumstantial evidence that the companion and disc may not share the same orbital plane. Whether the companion severely disrupts the disc, truncates it, or has little effect at all will require further observations of both the companion and disc. Show less
