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Obituary J. W. ten Cate
Huisman, M.V.; Levi, M.; Royen, E.A. van; Breederveld, C.; Buller, H.R.; Peters, M.; ... ; Agnelli, G. 2021
Obituary J. W. ten Cate
Other
open access
Accuracy and Reproducibility in Quantification of Plasma Protein Concentrations by Mass Spectrometry without the Use of Isotopic Standards
Kramer, G.J.; Woolerton, Y.; Straalen, J.P. van; Vissers, J.P.C.; Dekker, N.; Langridge, J.I.; ... ; Aerts, J.M.F.G. 2015
Accuracy and Reproducibility in Quantification of Plasma Protein Concentrations by Mass Spectrometry without the Use of Isotopic Standards
Article / Letter to editor
open access
Accuracy and Reproducibility in Quantification of Plasma Protein Concentrations by Mass Spectrometry without the Use of Isotopic Standards
Kramer, G.J.; Woolerton, Y.; Straalen, J.P. van; Vissers, J.P.C.; Dekker, N.; Langridge, J. I.; ... ; Aerts, J.M.F.G. 2015
Accuracy and Reproducibility in Quantification of Plasma Protein Concentrations by Mass Spectrometry without the Use of Isotopic Standards
Article / Letter to editor
open access
Comparative Study of Blood Collection Tubes and Thromboplastin Reagents for Correction of INR Discrepancies A Proposal for Maximum Allowable Magnesium Contamination in Sodium Citrate Anticoagulant Solutions
Besselaar, A.M.H.P. van den; Zanten, A.P. van; Brantjes, H.M.; Elisen, M.G.L.M.; Meer, F.J.M. van der; Poland, D.C.W.; ... ; Castel, A. 2012
Comparative Study of Blood Collection Tubes and Thromboplastin Reagents for Correction of INR Discrepancies A Proposal for Maximum Allowable Magnesium Contamination in Sodium Citrate Anticoagulant Solutions
Article / Letter to editor
metadata only
Correction of INR discrepancy between laboratories using various pt systems
Meer, F.J.M. van der; Besselaar, A.M. van den; Zanten, A.P. van; Brandjes, H.M.; Elisen, M.G.; Poland, D.C.; ... ; Castel, A. 2011
Correction of INR discrepancy between laboratories using various pt systems
Article / Letter to editor
metadata only
C-reactive protein in myocardial infarction binds to circulating microparticles but is not associated with complement activation
Zee, P.M. van der; Biro, E.; Trouw, L.A.; Ko, Y.; Winter, R.J. de; Hack, C.E.; ... ; Nieuwland, R. 2010
C-reactive protein in myocardial infarction binds to circulating microparticles but is not associated with complement activation
Article / Letter to editor
metadata only
Background: C-reactive protein (CRP) is elevated in patients with acute myocardial infarction (AMI). When CRP binds to membrane phospholipids or Fc receptors, it activates the complement system.... Show moreBackground: C-reactive protein (CRP) is elevated in patients with acute myocardial infarction (AMI). When CRP binds to membrane phospholipids or Fc receptors, it activates the complement system. Recent studies show that CRP can be exposed on cell-derived microparticles (MP) and is associated complement activation. Objectives. We studied complement activation on circulating MP in AMI patients and healthy controls. Methods. MP were isolated from plasma of AMI patients (n=21) and sex- and age-matched healthy individuals (n=10), and analyzed by flow cytometry for bound complement components (C1q, C4, C3) and complement inhibitor and activator molecules (C4bp, CRP, serum amyloid P component, immunoglobulins IgM and IgG). Concurrently, the levels of fluid phase complement activation products and inhibitor and activator molecules were determined Results: Fluid phase CRP, MP with bound CRP (CRP + MP), and C3 activation products were elevated in AMI patients compared to controls (P=0.032, P=0.031 and P=0.023, respectively), and fluid phase CRP correlated with CRP+ MP (r=0.84, P<0.001). Although CRP+ MP were elevated, they were not associated with C1q+ MP (r=0.32, P=0.174). In contrast, IgG+ MP were associated with C1q+ MP (r=0.73, P<0.001), C4+ MP and C3+ MP (r=0.78 and r=0.87, respectively, both P<0 001), and C4bp (r=0.63, P=0.004). In healthy individuals, CRP+ MP were strongly associated with C1q+ MP (r=0 82, P=0.007), which in turn were associated with C4+ MP and C3+ MP (r=0.68, P=0.032 and r=0.68, P=0.031, respectively). Conclusions: Despite CRP-associated complement activation on the surface of MP in healthy individuals and a strong correlation between MP-bound CRP and fluid phase CRP in AMI patients, the MP-associated complement activation is IgG- but not CRP-dependent in AMP patients (C) 2010 Elsevier Inc. All rights reserved. Show less