The narrow-headed vole, collared lemming and common vole were the most abundant small mammal species across the Eurasian Late Pleistocene steppe-tundra environment. Previous ancient DNA studies of... Show moreThe narrow-headed vole, collared lemming and common vole were the most abundant small mammal species across the Eurasian Late Pleistocene steppe-tundra environment. Previous ancient DNA studies of the collared lemming and common vole have revealed dynamic population histories shaped by climatic fluctuations. To investigate the extent to which species with similar adaptations share common evolutionary histories, we generated a dataset comprised the mitochondrial genomes of 139 ancient and 6 modern narrow-headed voles from several sites across Europe and northwestern Asia covering approximately the last 100 thousand years (kyr). We inferred Bayesian time-aware phylogenies using 11 radiocarbon-dated samples to calibrate the molecular clock. Divergence of the main mtDNA lineages across the three species occurred during marine isotope stages (MIS) 7 and MIS 5, suggesting a common response of species adapted to open habitat during interglacials. We identified several time-structured mtDNA lineages in European narrow-headed vole, suggesting lineage turnover. The timing of some of these turnovers was synchronous across the three species, allowing us to identify the main drivers of the Late Pleistocene dynamics of steppe- and cold-adapted species. Show less
The Late Pleistocene archeological record shows emerging patterns of population turnover frequently associated with technological change between c. 50–40 thousand years ago. In Europe, this is... Show moreThe Late Pleistocene archeological record shows emerging patterns of population turnover frequently associated with technological change between c. 50–40 thousand years ago. In Europe, this is thought to be related to indigenous population admixture and/or the diffusion of developing technologies by Homo sapiens resulting in a widely distributed spatiotemporal patchwork of industries with combinations of Middle and Upper Paleolithic traits. The Late Pleistocene record of Romania forms an anomaly in these scenarios. On the one hand, the country has important Pleistocene archives that preserve direct evidence of early modern humans with Neandertal genetic introgression. On the other hand, Romania shows no evidence of novel technology during the Middle to Upper Paleolithic transition. Here, we review the Late Middle and Early Upper Paleolithic archeological record of Romania supplemented with new radiocarbon ages and excavation data to clarify the validity of this current archeological interpretation. We conclude that while Neandertals and modern humans were in regional contact, raw materials eccentricities and incomplete empirical knowledge of past intergroup cultural transmission have obscured our ability to identify indicative material cultural signals indicating that current methods of understanding hybridized material culture are incomplete. Show less
Understanding Palaeolithic hominin subsistence strategies requires the comprehensive taxonomic identifcation of faunal remains. The high fragmentation of Late Pleistocene faunal assemblages often... Show moreUnderstanding Palaeolithic hominin subsistence strategies requires the comprehensive taxonomic identifcation of faunal remains. The high fragmentation of Late Pleistocene faunal assemblages often prevents proper taxonomic identifcation based on bone morphology. It has been assumed that the morphologically unidentifable component of the faunal assemblage would refect the taxonomic abundances of the morphologically identifed portion. In this study, we analyse three faunal datasets covering the Middle to Upper Palaeolithic transition (MUPT) at Bacho Kiro Cave (Bulgaria) and Les Cottés and La Ferrassie (France) with the application of collagen type I peptide mass fngerprinting (ZooMS). Our results emphasise that the fragmented component of Palaeolithic bone assemblages can difer signifcantly from the morphologically identifable component. We obtain contrasting identifcation rates between taxa resulting in an overrepresentation of morphologically identifed reindeer (Rangifer tarandus) and an underrepresentation of aurochs/bison (Bos/Bison) and horse/European ass (Equus) at Les Cottés and La Ferrassie. Together with an increase in the relative diversity of the faunal composition, these results have implications for the interpretation of subsistence strategies during a period of possible interaction between Neanderthals and Homo sapiens in Europe. Furthermore, shifts in faunal community composition and in carnivore activity suggest a change in the interaction between humans and carnivores across the MUPT and indicate a possible diference in site use between Neanderthals and Homo sapiens. The combined use of traditional and biomolecular methods allows (zoo)archaeologists to tackle some of the methodological limits commonly faced during the morphological assessment of Palaeolithic bone assemblages. Show less
Artefacts made from stones, bones and teeth are fundamental to our understanding of human subsistence strategies, behaviour and culture in the Pleistocene. Although these resources are plentiful,... Show moreArtefacts made from stones, bones and teeth are fundamental to our understanding of human subsistence strategies, behaviour and culture in the Pleistocene. Although these resources are plentiful, it is impossible to associate artefacts to specific human individuals(1) who can be morphologically or genetically characterized, unless they are found within burials, which are rare in this time period. Thus, our ability to discern the societal roles of Pleistocene individuals based on their biological sex or genetic ancestry is limited(2-5). Here we report the development of a non-destructive method for the gradual release of DNA trapped in ancient bone and tooth artefacts. Application of the method to an Upper Palaeolithic deer tooth pendant from Denisova Cave, Russia, resulted in the recovery of ancient human and deer mitochondrial genomes, which allowed us to estimate the age of the pendant at approximately 19,000-25,000 years. Nuclear DNA analysis identifies the presumed maker or wearer of the pendant as a female individual with strong genetic affinities to a group of Ancient North Eurasian individuals who lived around the same time but were previously found only further east in Siberia. Our work redefines how cultural and genetic records can be linked in prehistoric archaeology. Show less
We are a group of archaeologists, anthropologists, curators and geneticists representing diverse global communities and 31 countries. All of us met in a virtual workshop dedicated to ethics in... Show moreWe are a group of archaeologists, anthropologists, curators and geneticists representing diverse global communities and 31 countries. All of us met in a virtual workshop dedicated to ethics in ancient DNA research held in November 2020. There was widespread agreement that globally applicable ethical guidelines are needed, but that recent recommendations grounded in discussion about research on human remains from North America are not always generalizable worldwide. Here we propose the following globally applicable guidelines, taking into consideration diverse contexts. These hold that: (1) researchers must ensure that all regulations were followed in the places where they work and from which the human remains derived; (2) researchers must prepare a detailed plan prior to beginning any study; (3) researchers must minimize damage to human remains; (4) researchers must ensure that data are made available following publication to allow critical re-examination of scientific findings; and (5) researchers must engage with other stakeholders from the beginning of a study and ensure respect and sensitivity to stakeholder perspectives. We commit to adhering to these guidelines and expect they will promote a high ethical standard in DNA research on human remains going forward.In this Perspective, a group representing a range of stakeholders makes the case for a set of five proposed globally applicable ethical guidelines for ancient human DNA research. Show less
Nous sommes un groupe d'archéologues, d’anthropologues, de conservateurs et de généticiens de différentes origines et représentant 31 pays. Nous nous sommes rencontrés en novembre 2020 lors d'une... Show moreNous sommes un groupe d'archéologues, d’anthropologues, de conservateurs et de généticiens de différentes origines et représentant 31 pays. Nous nous sommes rencontrés en novembre 2020 lors d'une table ronde en ligne dédiée à l'éthique de la recherche d’ADN ancien. Nous avons alors convenu de la nécessité de lignes directrices éthiques applicables à l'échelle mondiale. Les recommandations publiées récemment et développées dans le cadre de recherches d’ADN sur des vestiges humains d’Amérique du Nord ne s’avèrent pas généralisables à d’autres régions du monde. Suite à l’analyse de divers contextes, nous proposons ici des lignes directrices que nous pensons être applicables partout dans le monde. Ces lignes directrices sont les suivantes : 1) les chercheurs doivent s'assurer que les règlements s’appliquant dans les lieux où ils travaillent ainsi que dans les lieux d’où proviennent les vestiges humains sont bien respectés ; 2) les chercheurs doivent préparer un programme de recherche détaillé avant de commencer toute étude ; 3) les chercheurs doivent minimiser les dommages causés aux vestiges humains ; 4) les chercheurs doivent s'assurer que les données seront disponibles après la publication pour permettre un réexamen critique des résultats scientifiques; et 5) les chercheurs doivent échanger avec toutes les parties impliquées dès le début de l’étude et respecter leurs points de vue. Nous nous engageons à respecter ces directives et nous souhaitons qu’elles contribuent au renforcement de la pratique éthique de notre champ de recherche. Show less
The recent elaboration and rapid expansion of aDNA, paleoproteomics, and related fields have propelled a profound "biomolecular turn" in archaeology and fundamentally changed the topology of... Show moreThe recent elaboration and rapid expansion of aDNA, paleoproteomics, and related fields have propelled a profound "biomolecular turn" in archaeology and fundamentally changed the topology of archaeological knowledge production. Such a transformation of the archaeological research landscape is not without consequence for long-standing research practices in the field, such as lithic analysis. This special issue derives from the session Old Stones, New Eyes? organized by the authors at the UISPP World Congress in Paris in 2018, which aimed to explore the future of lithic studies. An underlying theme of our session was the felt need to respond to the increasing marginalization of lithic research in terms of its capacity to (1) contribute to the grand narratives of early human evolution and (2) better articulate the role and significance of lithic studies in interdisciplinary human origins research. In this editorial, we briefly outline some of the questions and challenges raised by the biomolecular turn and advocate for a more self-conscious and reflexive stance among lithic experts. We argue that lithic studies fulfill all necessary requirements to act as a basic science for human origins research and that its role and status depends less on technological advances, such as, e.g., improved computing facilities, novel analytical software, or automated shape capture technologies, than on continuous work on the conceptual and methodological foundations of inquiry. We finally draw attention to the unique capability of lithic studies to shed light on the human technological condition and illustrate this potential by introducing and briefly discussing the papers included in this issue. Show less
The recent elaboration and rapid expansion of aDNA, paleoproteomics, and related fields have propelled a profound "biomolecular turn" in archaeology and fundamentally changed the topology of... Show moreThe recent elaboration and rapid expansion of aDNA, paleoproteomics, and related fields have propelled a profound "biomolecular turn" in archaeology and fundamentally changed the topology of archaeological knowledge production. Such a transformation of the archaeological research landscape is not without consequence for long-standing research practices in the field, such as lithic analysis. This special issue derives from the session Old Stones, New Eyes? organized by the authors at the UISPP World Congress in Paris in 2018, which aimed to explore the future of lithic studies. An underlying theme of our session was the felt need to respond to the increasing marginalization of lithic research in terms of its capacity to (1) contribute to the grand narratives of early human evolution and (2) better articulate the role and significance of lithic studies in interdisciplinary human origins research. In this editorial, we briefly outline some of the questions and challenges raised by the biomolecular turn and advocate for a more self-conscious and reflexive stance among lithic experts. We argue that lithic studies fulfill all necessary requirements to act as a basic science for human origins research and that its role and status depends less on technological advances, such as, e.g., improved computing facilities, novel analytical software, or automated shape capture technologies, than on continuous work on the conceptual and methodological foundations of inquiry. We finally draw attention to the unique capability of lithic studies to shed light on the human technological condition and illustrate this potential by introducing and briefly discussing the papers included in this issue. Show less
Fire use appears to have been relatively common among Neandertals in the Middle Palaeolithic. However, the means by which Neandertals procured their fire—either through the collection of natural... Show moreFire use appears to have been relatively common among Neandertals in the Middle Palaeolithic. However, the means by which Neandertals procured their fire—either through the collection of natural fire, or by producing it themselves using tools—is still a matter of debate. We present here the first direct artefactual evidence for regular, systematic fire production by Neandertals. From archaeological layers attributed to late Mousterian industries at multiple sites throughout France, primarily to the Mousterian of Acheulean Tradition (MTA) technoculture (ca. 50,000 years BP), we identify using microwear analysis dozens of late Middle Palaeolithic bifacial tools that exhibit macroscopic and microscopic traces suggesting repeated percussion and/or forceful abrasion with a hard mineral material. Both the locations and nature of the polish and associated striations are comparable to those obtained experimentally by obliquely percussing fragments of pyrite (FeS2) against the flat/convex sides of a biface to make fire. The striations within these discrete use zones are always oriented roughly parallel to the longitudinal axis of the tool, allowing us to rule out taphonomic origins for these traces. We therefore suggest that the occasional use of bifaces as ‘strike-a-lights’ was a technocultural feature shared among the late Neandertals in France. Show less
Hajdinjak, M.; Fu, Q.; Hübner, A.; Petr, M.; Mafessoni, F.; Grote, S.; ... ; Pääbo, S. 2018
Although it has previously been shown that Neanderthals contributed DNA to modern humans, not much is known about the genetic diversity of Neanderthals or the relationship between late Neanderthal... Show moreAlthough it has previously been shown that Neanderthals contributed DNA to modern humans, not much is known about the genetic diversity of Neanderthals or the relationship between late Neanderthal populations at the time at which their last interactions with early modern humans occurred and before they eventually disappeared. Our ability to retrieve DNA from a larger number of Neanderthal individuals has been limited by poor preservation of endogenous DNA and contamination of Neanderthal skeletal remains by large amounts of microbial and present-day human DNA. Here we use hypochlorite treatment of as little as 9 mg of bone or tooth powder to generate between 1- and 2.7-fold genomic coverage of five Neanderthals who lived around 39,000 to 47,000 years ago (that is, late Neanderthals), thereby doubling the number of Neanderthals for which genome sequences are available. Genetic similarity among late Neanderthals is well predicted by their geographical location, and comparison to the genome of an older Neanderthal from the Caucasus indicates that a population turnover is likely to have occurred, either in the Caucasus or throughout Europe, towards the end of Neanderthal history. We find that the bulk of Neanderthal gene flow into early modern humans originated from one or more source populations that diverged from the Neanderthals that were studied here at least 70,000 years ago, but after they split from a previously sequenced Neanderthal from Siberia around 150,000 years ago. Although four of the Neanderthals studied here post-date the putative arrival of early modern humans into Europe, we do not detect any recent gene flow from early modern humans in their ancestry. Show less
Deschamps, A.; Liard, M.; Chamaux, G.; Musch, J.; Guéret, C.; Hamon, C.; Soressi, M.A. 2018
Although a rich record of Pleistocene human-associated archaeological assemblages exists, the scarcity of hominin fossils often impedes the understanding of which hominins occupied a site. Using... Show moreAlthough a rich record of Pleistocene human-associated archaeological assemblages exists, the scarcity of hominin fossils often impedes the understanding of which hominins occupied a site. Using targeted enrichment of mitochondrial DNA, we show that cave sediments represent a rich source of ancient mammalian DNA that often includes traces of hominin DNA, even at sites and in layers where no hominin remains have been discovered. By automation-assisted screening of numerous sediment samples, we detected Neandertal DNA in eight archaeological layers from four caves in Eurasia. In Denisova Cave, we retrieved Denisovan DNA in a Middle Pleistocene layer near the bottom of the stratigraphy. Our work opens the possibility of detecting the presence of hominin groups at sites and in areas where no skeletal remains are found. Show less
Peptide mass fingerprinting of bone collagen (ZooMS) has previously been proposed as a method to calculate the extent of the non-enzymatic degradation of glutamine into glutamic acid (deamidation).... Show morePeptide mass fingerprinting of bone collagen (ZooMS) has previously been proposed as a method to calculate the extent of the non-enzymatic degradation of glutamine into glutamic acid (deamidation). Temporal and spatial variation of glutamine deamidation at a single site, however, has not been investigated. Here we apply ZooMS screening of Châtelperronian and Early Holocene bone specimens from Quinçay, France, to explore temporal and spatial variation in glutamine deamidation. Our results indicate that chronological resolution is low, while spatial variation is high. Nevertheless, our analysis allows the identification of bone specimens that have undergone diagenetic histories remarkably different (either in length or in type) from spatially related bone specimens. Therefore, ZooMS ammonium-bicarbonate screening is capable of testing bone assemblage homogeneity, which could guide subsequent analysis and interpretation. Show less
