Plant development is highly affected by light quality, direction, and intensity. Under natural growth conditions, shoots are directly exposed to light whereas roots develop underground shielded... Show morePlant development is highly affected by light quality, direction, and intensity. Under natural growth conditions, shoots are directly exposed to light whereas roots develop underground shielded from direct illumination. The photomorphogenic development strongly represses shoot elongation whereas promotes root growth. Over the years, several studies helped the elucidation of signaling elements that coordinate light perception and underlying developmental outputs. Light exposure of the shoots has diverse effects on main root growth and lateral root (LR) formation. In this study, we evaluated the phenotypic root responses of wild-type Arabidopsis plants, as well as several mutants, grown in a D-Root system. We observed that sucrose and light act synergistically to promote root growth and that sucrose alone cannot overcome the light requirement for root growth. We also have shown that roots respond to the light intensity applied to the shoot by changes in primary and LR development. Loss-of-function mutants for several root light-response genes display varying phenotypes according to the light intensity to which shoots are exposed. Low light intensity strongly impaired LR development for most genotypes. Only vid-27 and pils4 mutants showed higher LR density at 40 mu mol m(-2) s(-1) than at 80 mu mol m(-2) s(-1) whereas yuc3 and shy2-2 presented no LR development in any light condition, reinforcing the importance of auxin signaling in light-dependent root development. Our results support the use of D-Root systems to avoid the effects of direct root illumination that might lead to artifacts and unnatural phenotypic outputs. Show less
Dhonukshe, P.; Huang, F.; Galvan Ampudia, C.S.; Pekka Måhönen, A.; Kleine-Vehn, J.; Xu, J.; ... ; Offringa, R. 2015
The authors informed us of problems related to Fig. 3C and Fig. 7D in Development137, 3245-3255. Both issues were noted by an investigation by the Technical Committee of Utrecht University (UTC)... Show moreThe authors informed us of problems related to Fig. 3C and Fig. 7D in Development137, 3245-3255. Both issues were noted by an investigation by the Technical Committee of Utrecht University (UTC) into this publication. Based on the findings provided (detailed below), the journal has decided that the major conclusions of the paper are not affected and that retraction is not required, but that a correction should be provided with an explanation of the circumstances. This course of action complies with our policy on correction of issues in the scientific record, which states: “Should an error appear in a published article that affects scientific meaning or author credibility but does not affect the overall results and conclusions of the paper, our policy is to publish a Correction”.Development 2010 137: 3245-3255; doi: 10.1242/dev.052456 Show less
Dhonukshe, P.; Huang, F.; Galvan Ampudia, C.S.; Pekka Måhönen, A.; Kleine-Vehn, J.; Xu, J.; ... ; Offringa, R. 2010
