In bioarchaeology, the universal concept of “kinship” can be explored through the study of archaeological remains and as such offers a way to connect modern-day life to the life of past people.... Show moreIn bioarchaeology, the universal concept of “kinship” can be explored through the study of archaeological remains and as such offers a way to connect modern-day life to the life of past people. This study examines the social structure of post-medieval Dutch communities and their level of genetic homogeneity and inter-relatedness. The research aims were to identify probable genetic relatives within the Middenbeemster skeletal collection through developmental foot anomalies and to analyze the spatial structure of the Middenbeemster cemetery in the context of intracemetery kinship relations. Three hundred eighty individuals from four different skeletal collections were examined for the presence of fourteen non-metric traits, selected on various criteria (e.g., heritability). The Middenbeemster trait frequencies were compared with those of a reference sample of the post-medieval Dutch population (consisting of individuals from the Dutch post-medieval collections of Arnhem, Eindhoven, and Zwolle). A hypothetical kinship group could be identified when the trait frequencies of the Middenbeemster sample were considerably higher than those in the reference sample. Other sources had only limited validation value in relation to the hypothesis. Visual examination and spatial statistics of the distribution of the hypothetical kinship group revealed a possible patrilineally structured cemetery, although this is based on a small sample. By putting the observed trait frequencies in a broader context, the data suggested a rather high inter-relatedness of the Middenbeemster community. It also exposed the need for a better understanding of the used traits and perhaps a different approach to kinship analysis (due to necessarily large time investment in contrast to limited results). In conclusion, this study gave an insight into the social structure of post-medieval Dutch communities. Future improvements to kinship analysis may not only be beneficial for bioarchaeology, but also for other fields such as forensic anthropology. Show less
In the late medieval period, Holland experienced substantial socio-economic change. While the region was largely undeveloped prior to 1200 CE, the period after was characterised by extensive... Show moreIn the late medieval period, Holland experienced substantial socio-economic change. While the region was largely undeveloped prior to 1200 CE, the period after was characterised by extensive urbanisation and flourishing international trade, changes that would have impacted many aspects of life. This paper investigates the effect of these changes on diet by comparing skeletal collections from the early/central medieval rural village of Blokhuizen (800–1200 CE) to the late medieval urban town of Alkmaar (1448–1572 CE) using a combination of the prevalence and location of carious lesions (nteeth = 3475) and stable carbon and nitrogen isotope data (n = 50). Results show that the urban Alkmaar population had a significantly higher caries frequency (7.4% vs. 16.1%), starting at a younger age. Moreover, Alkmaar had significantly more approximal caries. These results point to increased consumption of cariogenic products, such as sugars and starches, by the urban citizens. Dietary differences are also demonstrated by the stable isotope data. Alkmaar individuals have significantly enriched δ15N ratios and more variable δ13C ratios compared with rural Blokhuizen. The elevated δ15N values may be due to increased consumption of fish or animals such as omnivorous pigs and chickens. The combination of caries and isotopic data points to clear changes in diet suggesting that urban individuals in the late medieval period had a substantially different diet compared with early rural inhabitants from the same area. Specifically, an increase in market dependence, availability of international trade products, and the growth of commercial fishing in the late medieval period may have contributed to this dietary shift. Future research should include a late medieval rural population to better understand the effects of late medieval socio-economic developments outside of the urban environment. This study demonstrates that the integration of palaeopathology and stable isotopic research provides a more complete understanding of dietary changes in medieval Holland. Show less
The site of Anse Trabaud on Martinique in the Windward Islands of the Lesser Antilles has yielded valuable information about human occupation from deep beneath its surface. The site is located in... Show moreThe site of Anse Trabaud on Martinique in the Windward Islands of the Lesser Antilles has yielded valuable information about human occupation from deep beneath its surface. The site is located in the southeastern part of the island, an area vulnerable to extreme wave events. The archaeological deposits are dated to 1290–780 cal BP (2σ). The earliest horizon is buried under thick layers of mangrove sediments. The deeply buried deposits have allowed excellent preservation of inorganic and organic remains providing an unexpected opportunity to reconstruct the paleoenvironmental conditions of past human settlement, and to study human-environment interactions and social adaptation to climate challenges in this part of the Caribbean archipelago. The cultural remains provide invaluable information into the exploitation of the surroundings of the site as well as into the subsistence patterns, material culture repertoires, procurement strategies and use and exchange of raw materials and tools. Additionally, the excellent preservation of organic materials offers new insights into food plants grown and harvested on-site, and then processed and consumed. The environmental challenges incited the Anse Trabaud community to adapt their settlement organisation and procurement strategies over time and underscore the importance of their participation in a regional and social network of mobility and exchange. Show less
ObjectivesThe objective of this study is to apply pubertal stage estimation methods to a sample from a rural community: the post‐medieval Dutch skeletal collection from Middenbeemster. Puberty is a... Show moreObjectivesThe objective of this study is to apply pubertal stage estimation methods to a sample from a rural community: the post‐medieval Dutch skeletal collection from Middenbeemster. Puberty is a key developmental period involving transition to physical adulthood with broad societal relevance through its impact on fertility, morbidity, and mortality.Materials and methodsIndividuals (n = 55), including 27 of known sex and age‐at‐death, between the ages of 8 and 25 years were assessed for six skeletal markers indicative of pubertal growth spurt. Recent novel osteoarchaeological methods from Shapland and Lewis are used to reconstruct the timing and duration of pubertal stages.ResultsPubertal acceleration occurred earlier in females (10.38 years, n = 8) than males (13.30 years, n = 6), whereas maturation occurred later in males (21.36 years, n = 11) than females (19.30 years, n = 5). Onset appears earlier and completion later compared to other archaeological skeletal samples with osteoarchaeological evidence of puberty. Age shortly after menarche was reconstructed at 20.45 years, substantially later than historic records and bioarchaeological research reports suggest.ConclusionThis early onset and late completion caused a “stretch” of the overall duration of puberty compared to other collections, especially of the last three stages. This prolonged development is reflected in historically known social expectations for the Netherlands, for example, that marriage and children should not occur before about 22–23 years of age. Increasing the range of past peoples with puberty stage reconstruction will permit more insightful interpretations of the biological and cultural patterns of this important life stage. Show less
The European conquest and colonization of the Caribbean precipitated massive changes in indigenous cultures and societies of the region. One of the earliest changes was the introduction of new... Show moreThe European conquest and colonization of the Caribbean precipitated massive changes in indigenous cultures and societies of the region. One of the earliest changes was the introduction of new plant and animal foods and culinary traditions. This study presents the first archaeological reconstruction of indigenous diets and foodways in the Caribbean spanning the historical divide of 1492. We use multiple isotope datasets to reconstruct these diets and investigate the potential relationships between dietary and mobility patterns at multiple scales. Dietary patterns are assessed by isotope analyses of different skeletal elements from the archaeological skeletal population of El Chorro de Maita, Cuba. This approach integrates carbon and nitrogen isotope analyses of bone and dentine collagen with carbon and oxygen isotope analyses of bone and enamel apatite. The isotope results document extreme intrapopulation dietary heterogeneity but few systematic differences in diet between demographic/social groups. Comparisons with published isotope data from other precolonial and colonial period populations in the Caribbean indicate distinct dietary and subsistence practices at El Chorro de Maita. The majority of the local population consumed more animal protein resources than other indigenous populations in the Caribbean, and their overall dietary patterns are more similar to colonial period enslaved populations than to indigenous ones. Show less
European enslavement of Indigenous peoples in the Americas began in the Caribbean, quickly spreading to the rest of the continent and impacting the lives of millions. Despite its centrality to the... Show moreEuropean enslavement of Indigenous peoples in the Americas began in the Caribbean, quickly spreading to the rest of the continent and impacting the lives of millions. Despite its centrality to the creation of the colonial Caribbean, is still an understudied subject. This article summarizes the archaeological evidence on the topic and discusses the utility of the archaeological approach based on research conducted at the Cuban site of El Chorro de Maíta. The analyses of diet and paleodemography indicate substantial changes when compared to precolonial Indigenous populations. Indicators of ethnic diversity and geographic origin, as well as the mortuary patterns and distribution of material culture help to identify the presence of slaves. Show less
Bartholdy, B.P.; Hoogland, M.L.P.; Waters‐Rist, A. 2019
The main aim of this study is to present a novel method of nonadult (ca. 1–19 years) age‐at‐death estimation using the dental wear of deciduous, mixed deciduous‐permanent, and permanent dentitions... Show moreThe main aim of this study is to present a novel method of nonadult (ca. 1–19 years) age‐at‐death estimation using the dental wear of deciduous, mixed deciduous‐permanent, and permanent dentitions, including the incisors, canines, premolars, and first and second molars. The stage‐based method is derived from degrees of dental wear in known‐age (n = 39) and estimated‐age (n = 11) nonadults containing 951 teeth from the predominately 19th century cemetery of Middenbeemster, The Netherlands. The need for such a method is warranted in cases where dental development and/or eruption cannot be assessed for age‐at‐death estimation. As well, by establishing a baseline for normal age‐related nonadult tooth wear, users may better document wear that could be due to extramasticatory behaviours. The regression analysis reveals a strong quadratic correlation—F(2, 47) = 555.1, p < .001, R2 = .95, standard error of the estimate = 1.14, residual sum of squares (RSS) = 68.89, predicted residual error sum of squares (PRESS) = 77.67—between age and wear and multivariate adaptive regression splines (R2 = .95, generalised cross validation = 1.67, RSS = 67.68, PRESS = 89.34), which are used to develop an R‐package that users may employ to estimate age‐at‐death from dental wear. The accuracy of this method (78–98%) is evaluated using leave‐one‐out cross‐validation. Analyses of males versus females, deciduous versus permanent, upper versus lower, and anterior versus posterior teeth revealed no apparent reason to warrant separate methods for these groups of separated dentitions. This method fills a disciplinary gap in the understudied area of deciduous and nonadult dental wear and hopes to stimulate much future research. With the R‐package, we also provide the foundation and framework for the development of additional reference populations across different spatiotemporal contexts, to make the method more widely applicable. Show less
ObjectivesDental calculus is among the richest known sources of ancient DNA in the archaeological record. Although most DNA within calculus is microbial, it has been shown to contain sufficient... Show moreObjectivesDental calculus is among the richest known sources of ancient DNA in the archaeological record. Although most DNA within calculus is microbial, it has been shown to contain sufficient human DNA for the targeted retrieval of whole mitochondrial genomes. Here, we explore whether calculus is also a viable substrate for whole human genome recovery using targeted enrichment techniques. Materials and methodsTotal DNA extracted from 24 paired archaeological human dentin and calculus samples was subjected to whole human genome enrichment using in‐solution hybridization capture and high‐throughput sequencing. ResultsTotal DNA from calculus exceeded that of dentin in all cases, and although the proportion of human DNA was generally lower in calculus, the absolute human DNA content of calculus and dentin was not significantly different. Whole genome enrichment resulted in up to four‐fold enrichment of the human endogenous DNA content for both dentin and dental calculus libraries, albeit with some loss in complexity. Recovering more on‐target reads for the same sequencing effort generally improved the quality of downstream analyses, such as sex and ancestry estimation. For nonhuman DNA, comparison of phylum‐level microbial community structure revealed few differences between precapture and postcapture libraries, indicating that off‐target sequences in human genome‐enriched calculus libraries may still be useful for oral microbiome reconstruction. DiscussionWhile ancient human dental calculus does contain endogenous human DNA sequences, their relative proportion is low when compared with other skeletal tissues. Whole genome enrichment can help increase the proportion of recovered human reads, but in this instance enrichment efficiency was relatively low when compared with other forms of capture. We conclude that further optimization is necessary before the method can be routinely applied to archaeological samples. Show less
Hooghiemstra, H.; Olijhoek, T.; Hoogland, M.L.P.; Prins, M.; Geel, B. van; Donders, T.; ... ; Hofman, C.L. 2018
Columbus’ arrival in the New World in AD 1492 on the northern coast of Hispaniola was followed by a suite of changes in land-use. We reconstruct environmental change from a 225-cm-long sediment... Show moreColumbus’ arrival in the New World in AD 1492 on the northern coast of Hispaniola was followed by a suite of changes in land-use. We reconstruct environmental change from a 225-cm-long sediment core from site Los Indios from an abandoned and sediment-filled meander of the Yaque River, Cibao Valley, northeastern Dominican Republic. The sediment record starts ca. AD 195 (ca. 1755 cal. yr BP) and the history of the meander infill was monitored by changing grain size distributions, organic matter concentration and pollen from wetland plants. From ca. AD 200 to ca. AD 1525, the pollen record indicates a diverse forest assemblage; however, the presence of pollen from potential crop plants suggest nearby small-scale subsistence crop cultivation. More abundant charcoal after ca. AD 1410 shows Amerindians increasingly used fire. The record of grain size distributions shows that the meander was temporarily part of a low energetic drainage system in which bedload and suspended sediments accumulated. After European colonization of Hispaniola increasing spores of coprophilous fungi evidence that Europeans had introduced during the first decades of colonization cattle in the Cibao Valley which gradually resulted in more open forest. The charcoal record around ca. AD 1650 reflects intensive forest clearing, suggesting that small-scale Pre-Colonial practice of crop cultivation became replaced by large-scale agriculture on the moist and nutrient rich soils along the Yaque River. Further deforestation and signals of erosion suggest that the population of colonists and introduced enslaved labour force must have increased rapidly. After ca. AD 1740 charcoal influx decreased suggesting that last deforestation activities used selective cutting to produce fire wood and timber for construction, rather than burning forest in situ. Two centuries after European colonization, by the 18th century, land-use within the Cibao Valley had become a balance between substantial livestock and crop cultivation (pollen grains have evidenced cereals, maize, and potentially also sugar cane, amaranthaceous crops and tobacco). After ca. AD 1950, swamp vegetation of Typha and Cyperaceae decreased, pointing to an almost fully terrestrialized meander with only few bodies of standing water, reflecting the present-day setting. This multiproxy reconstruction of anthropogenic environmental change shows a clear differentiation between an immediate introduction of livestock and after some 150 years the development of a European style agriculture, providing a context for archaeological investigations. Show less
Dental calculus (calcified dental plaque) is prevalent in archaeological skeletal collections and is a rich source of oral microbiome and host-derived ancient biomolecules. Recently, it has been... Show moreDental calculus (calcified dental plaque) is prevalent in archaeological skeletal collections and is a rich source of oral microbiome and host-derived ancient biomolecules. Recently, it has been proposed that dental calculus may provide a more robust environment for DNA preservation than other skeletal remains, but this has not been systematically tested. In this study, shotgun-sequenced data from paired dental calculus and dentin samples from 48 globally distributed individuals are compared using a metagenomic approach. Overall, we find DNA from dental calculus is consistently more abundant and less contaminated than DNA from dentin. The majority of DNA in dental calculus is microbial and originates from the oral microbiome; however, a small but consistent proportion of DNA (mean 0.08 ± 0.08%, range 0.007–0.47%) derives from the host genome. Host DNA content within dentin is variable (mean 13.70 ± 18.62%, range 0.003–70.14%), and for a subset of dentin samples (15.21%), oral bacteria contribute > 20% of total DNA. Human DNA in dental calculus is highly fragmented, and is consistently shorter than both microbial DNA in dental calculus and human DNA in paired dentin samples. Finally, we find that microbial DNA fragmentation patterns are associated with guanine-cytosine (GC) content, but not aspects of cellular structure. Show less
