Background: Until recently, metronidazole was the first-Line treatment for Clostridioides difficile infection and it is still commonly used. Though resistance has been reported due to the plasmid... Show moreBackground: Until recently, metronidazole was the first-Line treatment for Clostridioides difficile infection and it is still commonly used. Though resistance has been reported due to the plasmid pCD-METRO, this does not explain all cases.Objectives: To identify factors that contribute to plasmid-independent metronidazole resistance of C. difficile.Methods: Here, we investigate resistance to metronidazole in a collection of clinical isolates of C. difficile using a combination of antimicrobial susceptibility testing on different solid agar media and WGS of selected isolates.Results: We find that nearly all isolates demonstrate a haem-dependent increase in the MIC of metronidazole, which in some cases Leads to isolates qualifying as resistant (MIC >2 mg/L). Moreover, we find an SNP in the haem-responsive gene hsmA, which defines a metronidazole-resistant Lineage of PCR ribotype 010/MLST ST15 isolates that also includes pCD-METRO-containing strains.Conclusions: Our data demonstrate that haem is crucial for medium-dependent metronidazole resistance in C. difficile. Show less
We share many of the views of de Groot et al. on the relevance of ecosystem services (ES) and the constructive role they have played in highlighting the importance of nature to people. Here we aim... Show moreWe share many of the views of de Groot et al. on the relevance of ecosystem services (ES) and the constructive role they have played in highlighting the importance of nature to people. Here we aim to further clarify how the concept of Nature’s Contribution to People (NCP) contributes to science and policy.It was not the aim of our article to review the literature on ecosystem services (ES). The point of our article was to explain the concept of NCP not to review the extensive ES literature. We are in full agreement that the influence of ES has been long and rich, from its first mention in the peer-reviewed literature (1) to the present. As explicitly stated in our articles (2, 3) and further clarified in our figure S1, the IPBES approach owes much to the influence of the Millennium Ecosystem Assessment (MA) (4). The NCP framing has a number of elements that were present in the MA, as well as new elements.Ecosystem services are a subset of NCP, but there is more to NCP than ES. Beyond apparent similarities in definitions (e.g. services = contributions in some cases), the ES and NCP framings are different, with NCP being epistemologically, ontologically and methodologically more pluralistic. ES are part of NCP, that is, the ES approach represents an important subset of ways to understand nature’s diverse contributions to people. Show less
Díaz, S.; Pascual, U.; Stenseke, M.; Martín-López, B.; Watson, R.T.; Molnár, Z.; ... ; Shirayama, Y. 2018
Daniel Faith makes several very good points. We deal with only one of them in this short response, the role of biodiversity as a source of options for people now and in the future. We agree with... Show moreDaniel Faith makes several very good points. We deal with only one of them in this short response, the role of biodiversity as a source of options for people now and in the future. We agree with Faith that biodiversity at all scales, from local to global, is critically important for humans in the face of the unknown, and for the future production of nature’s contributions to people (NCP). We also agree that NCP in the face of the unknown should include both those associated with particular components of biodiversity, and those related to the continued existence of the variety of life. One of our categories of NCP indeed addresses these two aspects. “Maintenance of options” (NCP 18 within the generalizing perspective, Table S1) refers to the capacity of ecosystems, habitats, species or genotypes to keep options open in order to support a good quality of life. This includes the future benefits or threats derived from particular genes, organisms, groups of organisms or ecosystems, be they still unknown or already known but their future uses as yet undiscovered. NCP 18 also includes the contributions of all species, populations and genotypes to processes for coping with environmental uncertainty, such as the resilience and resistance of ecosystems in the face of environmental change and variability. Finally, it recognises future benefits or threats that may be anticipated from ongoing biological evolution, including rapid contemporary evolution. Using the eloquent metaphor of Faith et al., the existing global variety of life is a “storehouse”, and the on-going evolutionary process is a “factory” of new benefits and threats; both are included in the NCP framework. The NCP approach also seeks to integrate the various ways in which nature keeps our options open through the lens of other knowledge systems (context-specific perspective), from contributing to food autonomy at the household and local levels, to mutual enhancement of regional crop diversity and social networks, to the deliberate dispersal along “song-lines” of nutritious non-cultivated species enhancing the liveability of forests for future generations. Show less
Díaz, S.; Pascual, U.; Stenseke, M.; Martín-López, B.; Watson, R.T.; Molnár, Z.; ... ; Shirayama, Y. 2018
A major challenge today and into the future is to maintain or enhance beneficial contributions of nature to a good quality of life for all people. This is among the key motivations of the... Show moreA major challenge today and into the future is to maintain or enhance beneficial contributions of nature to a good quality of life for all people. This is among the key motivations of the Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services (IPBES), a joint global effort by governments, academia, and civil society to assess and promote knowledge of Earth's biodiversity and ecosystems and their contribution to human societies in order to inform policy formulation. One of the more recent key elements of the IPBES conceptual framework (1) is the notion of nature's contributions to people (NCP), which builds on the ecosystem service concept popularized by the Millennium Ecosystem Assessment (MA) (2). But as we detail below, NCP as defined and put into practice in IPBES differs from earlier work in several important ways. First, the NCP approach recognizes the central and pervasive role that culture plays in defining all links between people and nature. Second, use of NCP elevates, emphasizes, and operationalizes the role of indigenous and local knowledge in understanding nature's contribution to people. Show less
