Palaeolithic faunal assemblages provide a wealth of information, notably on paleoenvironment, site formation and past human subsistence strategies. However, obtaining a secure interpretation... Show morePalaeolithic faunal assemblages provide a wealth of information, notably on paleoenvironment, site formation and past human subsistence strategies. However, obtaining a secure interpretation requires the comprehensive taxonomic identification of faunal remains, traditionally done through visual morphological comparison. Due to high fragmentation, bone morphology is often insufficient to taxonomically identify a large proportion of faunal assemblages. Zooarchaeology by Mass Spectrometry (ZooMS) gives the opportunity to identify biological markers preserved in previously undiagnostic fragmentary or worked remains. This dissertation combines the analyses of bone surface modifications with biomolecular analyses in order to investigate the relationship between faunal composition and bone fragmentation, and to assess hominin subsistence behaviour through large-scale applications of untargeted ZooMS screenings. This study explores the synthesis and analysis of comparable data, for both the morphologically identifiable and unidentifiable portion of the same faunal assemblages, by investigating bone assemblages from European sites spanning the arrival of Homo sapiens within territories occupied by the last Neanderthals such as Fumane Cave (Italy), Bacho Kiro Cave (Bulgaria), La Ferrassie and Les Cottés (France). In addition, the recent development of non-destructive proteomic extraction techniques, notably on fragmented Palaeolithic worked bones, required a controlled sampling experiment to determine how sampling techniques such as the eraser extraction method affect ancient bone surfaces at a microscopic level. Through the combination of methods and the integration of complementary datasets, this dissertation demonstrates the potential of the inclusion of ancient protein analysis within the framework of zooarchaeological analysis at Palaeolithic sites. Show less
Direct radiocarbon dating of human remains is crucial for the accurate interpretation of prehistory. Yet given the scarcity of prehistoric human remains, direct dating is often too destructive for... Show moreDirect radiocarbon dating of human remains is crucial for the accurate interpretation of prehistory. Yet given the scarcity of prehistoric human remains, direct dating is often too destructive for important fossils. The reduction of sample size necessary for dating bone is therefore of great interest to archaeologists, but the confounding factors of molecular preservation and contamination present great challenges to the radiocarbon dating community.This dissertation explores the reduction of sample size for radiocarbon dating Palaeolithic bone at the pretreatment and 14C measurement stages. Methodological tests were carried out on a selection of archaeological bones spanning the breadth of the radiocarbon method at varying levels of preservation. Our standard pretreatment protocol for ~500 mg bone was refined for <100 mg bone. Collagen extracted from solid pieces of bone (rather than powdered bone) and a reduced duration of the gelatinisation stage improved collagen yields for small samples. The quality of the extracted collagen was evaluated based on the yield, elemental and stable isotopic values and the obtained 14C measurements. Show less