Cyclic peptides are investigated as a platform to induce different orientations between various ligands. Through click chemistry, a variety of Toll-like receptor ligands can be attached to the... Show moreCyclic peptides are investigated as a platform to induce different orientations between various ligands. Through click chemistry, a variety of Toll-like receptor ligands can be attached to the cyclic peptides, which are based on gramicidin S. Show less
The aim of this thesis is to study cell-cell interactions and the development of an assay to explore and quantify the exchange of membrane compounds. In the first part the rate of lipid exchange... Show moreThe aim of this thesis is to study cell-cell interactions and the development of an assay to explore and quantify the exchange of membrane compounds. In the first part the rate of lipid exchange was studied, during both natural cell-cell contacts and prolonged enforced contacts. In the second part lipidated coiled-coil peptides were used to study the behaviour and potential fusogenic behaviour of these compounds on cell-wall-deficient bacteria. Show less
The aim of this thesis is to explore the use of Bioorthogonal Antigens to study the cross-presentation pathway. Bioorthogonal Antigens are antigens carrying bioorthogonal groups in specific... Show moreThe aim of this thesis is to explore the use of Bioorthogonal Antigens to study the cross-presentation pathway. Bioorthogonal Antigens are antigens carrying bioorthogonal groups in specific amino acid positions within the epitope region that can be reacted selectively within/on the cell using bioorthogonal ligation strategies. Incorporation of bioorthogonal groups into antigens has an advantage over other methods because most of the groups are stable to proteolysis and are small enough to have a minimal impact on routing and loading onto MHC-I molecules. In the future, these antigens have potential to be applied for imaging of the entire cross-presentation pathway using a single bioorthogonal handle. Show less
