This thesis describes the design and synthesis of fragments of various cell wall carbohydrates of the Streptococcus species, including the branched Group B-specific antigen (GBC) of Group B... Show moreThis thesis describes the design and synthesis of fragments of various cell wall carbohydrates of the Streptococcus species, including the branched Group B-specific antigen (GBC) of Group B Streptococcus, glycerol phosphate (GroP) modified group A carbohydrate (GAC), and the O-acetylated type 1 capsular polysaccharide of Streptococcus pneumonia (Sp1). All the synthesized fragments were equipped with a spacer at the reducing end for further conjugation with proteins or active small molecules to explore the mechanisms of carbohydrate-based vaccines in immune responses and to develop novel vaccines. To investigate the structure-activity relationship, several fragments of each polysaccharide were assembled varying in length. Show less
The objective of this study was to investigate the expression and function of GRHL2 in different breast cancer subtypes. In Chapter 2, we focused on the expression of GRHL2 in human breast cancer... Show moreThe objective of this study was to investigate the expression and function of GRHL2 in different breast cancer subtypes. In Chapter 2, we focused on the expression of GRHL2 in human breast cancer and the distinct effects of GRHL2 knockout on aspects of growth versus migration in basal A and luminal-like subtypes. In Chapter 3, ChIP-seq was used to explore the genomic landscape of GRHL2 binding sites in basal A and luminal-like subtypes of breast cancer and this data was used to predict shared and distinct GRHL2 target genes. In Chapter 4, based on a conditional GRHL2 knockout system, we determined the dynamic changes in genome-wide DNA transcription triggered by loss of GRHL2 in luminal-like breast cancer cells and used the data to predict affected pathways. In Chapter 5, ChIP-seq and BrU-seq data were integrated to identify genes whose transcription is controlled by GRHL2 and establish gene expression networks regulated by GRHL2 in luminal-like breast cancer. Show less
This thesis, from a comparative perspective, examines the worldview and its interplay to the frontier and military policies in early periods of the two ancient empires on Eurasian: Rome and Han... Show moreThis thesis, from a comparative perspective, examines the worldview and its interplay to the frontier and military policies in early periods of the two ancient empires on Eurasian: Rome and Han China. The first part (chapter 1 and chapter 2) concerns the formation and transformation of the world views of Rome and Qin-Han China in their respectively lengthy trajectories of empire-building. It is followed by part two (chapter 3 and chapter 4) in which I shift my focus from ideology to practical issues in attempt to observe how the distinctive world views held by the elite class of Rome and China were manifested and interacted with the actual policy-making and territorial conquests. The last part of the dissertation (chapter 5 and chapter 6) concentrates on the roles of emperors. By highlighting the distinctive roles that the Roman and Chinese emperors had played, it allows audience to have a better understanding in the similarities, and especially the crucial differences concerning the perception of the world and actual policies discussed above. Also, I hope this tentative comparative study sheds some lights on the reflection of the concept of empire in current scholarship of ancient history. Show less
