This thesis describes the design and synthesis of fragments of various cell wall carbohydrates of the Streptococcus species, including the branched Group B-specific antigen (GBC) of Group B... Show moreThis thesis describes the design and synthesis of fragments of various cell wall carbohydrates of the Streptococcus species, including the branched Group B-specific antigen (GBC) of Group B Streptococcus, glycerol phosphate (GroP) modified group A carbohydrate (GAC), and the O-acetylated type 1 capsular polysaccharide of Streptococcus pneumonia (Sp1). All the synthesized fragments were equipped with a spacer at the reducing end for further conjugation with proteins or active small molecules to explore the mechanisms of carbohydrate-based vaccines in immune responses and to develop novel vaccines. To investigate the structure-activity relationship, several fragments of each polysaccharide were assembled varying in length. Show less
The objective of this study was to investigate the expression and function of GRHL2 in different breast cancer subtypes. In Chapter 2, we focused on the expression of GRHL2 in human breast cancer... Show moreThe objective of this study was to investigate the expression and function of GRHL2 in different breast cancer subtypes. In Chapter 2, we focused on the expression of GRHL2 in human breast cancer and the distinct effects of GRHL2 knockout on aspects of growth versus migration in basal A and luminal-like subtypes. In Chapter 3, ChIP-seq was used to explore the genomic landscape of GRHL2 binding sites in basal A and luminal-like subtypes of breast cancer and this data was used to predict shared and distinct GRHL2 target genes. In Chapter 4, based on a conditional GRHL2 knockout system, we determined the dynamic changes in genome-wide DNA transcription triggered by loss of GRHL2 in luminal-like breast cancer cells and used the data to predict affected pathways. In Chapter 5, ChIP-seq and BrU-seq data were integrated to identify genes whose transcription is controlled by GRHL2 and establish gene expression networks regulated by GRHL2 in luminal-like breast cancer. Show less
What do elections mean for a single-party regime? Can party-selected deputies do something meaningful for citizens in non-democracies? The dissertation explores how and under what conditions a... Show moreWhat do elections mean for a single-party regime? Can party-selected deputies do something meaningful for citizens in non-democracies? The dissertation explores how and under what conditions a single-party authoritarian regime instrumentalizes popularly elected congress to strengthen its rule in local society. It takes contemporary China as an empirical focus for this exploration. Instead of perceiving Chinese congress either simply as authoritarian window dressing or as an immediate catalyst for democratization, this research is devoted to examining the motivations, strategies, and behaviors of the party regime in playing the cards of congressional elections and representation to make its rule more robust and resilient. This project mainly adopts comparative case study methods, with some quantitative data serving as supportive statistical evidence. As a whole, my thesis argues that congressional election and post-election representation are two cards of China’s party regime. By strategically downplaying input electoral competition but promoting output congressional representation, the communist regime has been striving to develop a mass-line democracy as an alternative to liberal democracy, which features the “Leninist trinity” of the Party’s leadership, the rule of law, and people’s democracy, as well as a new brand of mobilized representation relying on the accountability from the top down. Show less
