The qualitative and quantitative analysis of aflatoxin B-1 in a model system (water/ethanol), in different wines and in beer using one- and two-photon-induced fluorescence is discussed. The... Show moreThe qualitative and quantitative analysis of aflatoxin B-1 in a model system (water/ethanol), in different wines and in beer using one- and two-photon-induced fluorescence is discussed. The absorption and fluorescence properties of aflatoxin B-1 depend on the solvent and pH. The two-photon-absorption cross-section was calculated for aflatoxin B-1 in beer and wine (sigma (2) similar to 25 GM) for excitation at 720 nm. A comparison of the one- and two-photon- induced fluorescence results showed that the disturbance due to background emission originating from matrix constituents is significantly reduced under two-photon-excitation conditions. The limit of detection for the one- and two-photon-induced fluorescence was determined. Show less
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